Zambia Signal Functions study 2016 dataset

This dataset contains information related to health facilities’ infrastructure, staffing, equipment, supplies, and capacity to perform various clinical functions related to reproductive and maternal health service provision. The study was conducted in Central Province, Zambia and its primary aim was to assess facilities’ capacity to provide termination of pregnancy services. EMBARGOED UNTIL 31st DEC 201

Monodisperse Low-Bandgap Macromolecule-Based 5,5′-Bibenzo[c][1,2,5]thiadiazole Swivel Cruciform for Organic Solar Cells

A novel low bandgap star-like macromolecule was synthesized and applied as electron donor material in the bulk heterojunction solar cells, in which the 5,5′-bibenzo­[c]­[1,2,5]­thiadiazole was used as the central node, in conjunction with four conjugated donor–acceptor arms. Compared with the corresponding small molecule with first generation arms, the macromolecule with second generation branches exhibited significantly enhanced photovoltaic device performances (blended with PC71BM as the active layer) due to dramatically improved short-circuit current density (<i>J</i>_sc) and fill factor (FF). The improvement in <i>J</i>_sc and FF can be attributed to the more broad absorption and the more favorable phase separation when comparing a monodisperse macromolecule with the second generation arms (SFTBT) with a small molecule with first generation branches (DFTBT)

Luciferase reporter assay to validate the miRNA-mRNA relationship of miR-499-5p and DSTN.

<p>The analysis of the relative luciferase activity in PK-15 cells following transfection with candidate miRNA mimics. (A)Wild type 3’UTR dual luciferase report vectors were co-transfected with miRNA mimics, which caused a significant decrease in the relative luciferase activity compared with the negative control. (B) Mutated 3’UTR dual luciferase report vectors were co-transfected with miRNA mimics, which did not cause a decrease of the relative luciferase activity compared with the negative control. (C) Predicted target sites of miRNAs and the 4 bp mutation site (red) of genes’ mut-3’UTR. The error bars indicate the SEM. The significance of the differences was calculated using two-tailed T-test. N = 6. **, P<0.01.</p

Principal component analysis (PCA) plot of sequencing data in the ten libraries.

<p>Clustering of the ten libraries. The rhombuses represent the Tongcheng and the circles represent the Yorkshire breed. TC: Tongcheng, YK: Yorkshire. Stages: 40, 55, 63, 70, and 90 days post coitum (dpc).</p

Hierarchical clustering based on the expression of miRNAs.

<p>Clustering of the samples was determined by the expression of miRNAs. Red indicates high expression, while green indicates low expression. The expression values were normalized TPM of miRNA using the Z-score. TC: Tongcheng, YK: Yorkshire. Stages: 40, 55, 63, 70, and 90 days post coitum (dpc).</p

Interaction of breed-DE miRNAs and breed-DE mRNAs.

<p>The network was constructed using 36 hub miRNAs and 14 hub mRNAs from the pairs of breed-DE miRNAs and breed-DE mRNAs. The green diamonds represent miRNAs and the red circles represent mRNAs. The nodes with bigger size had higher degree. TC: Tongcheng, YK: Yorkshire. Stages: 40, 55, 63, 70, and 90 days post coitum (dpc).</p

KEGG orthology enriched for target genes of breed-DE miRNAs between TC and YK.

<p>KEGG orthology enriched for target genes of breed-DE miRNAs between TC and YK.</p

Comparison of differentially expressed (DE) miRNAs among five data sets.

<p>The number marked in the overlapping areas shows the common breed-DE miRNAs.TC: Tongcheng, YK: Yorkshire. Stages: 40, 55, 63, 70, and 90 days post coitum (dpc).</p

STEM clustering based on differentially expressed miRNAs.

<p>Each box corresponds to a kind of expression profile and only colored profiles reach statistical significance. The upper-left number in the box gives information about the order of profile. The bottom-left number gives information about the statistical significance. The right panel displays the patterns of miRNA expression for the “Cluster 0” profile in detail. A: Short time-series expression profiles of miRNAs in TC; B: Short time-series expression profiles of miRNAs in YK. TC: Tongcheng, YK: Yorkshire.</p