miR-203 was downregulated in human osteosarcoma cell lines and tissues.

<p>(A) qRT-PCR was performed to detect the miR-203 levels in human osteosarcoma cell lines (MG-63, SOSP-9607, SAOS-2, and U2OS) and one normal osteoblast cell line (hFOB). (B) qRT-PCR was performed to detect the miR-203 levels in osteosarcoma tissues and their corresponding nontumor tissues. (C) The relative expression of miR-203 was downregulated in osteosarcoma tissues compared with their corresponding nontumor tissues.</p

RAB22A overexpression blocks the roles of miR-203.

<p>(A) qRT-PCR was performed to detect the RAB22A levels in human osteosarcoma cell lines (MG-63, SOSP-9607, SAOS-2, and U2OS) and one normal osteoblast cell line (hFOB). (B) qRT-PCR was performed to detect the RAB22A levels in osteosarcoma tissues and their corresponding nontumor tissues. (C) The protein level of RAB22A was measured using western blot. (D) CCK8 assay showed that overexpression of RAB22A increased the miR-203 overexpressing MG-63 cells proliferation. (E) The mRNA expression of E-cadherin, N-cadherin and Vimentin was measured by using qRT-PCR. (F) The migration abilities of miR-203 overexpressing MG-63 cells were increased after RAB22A vector transfection.*p<0.05 and ***p<0.001.</p

Overexpression of miR-203 inhibited reversion of EMT.

<p>(A) Overexpression of miR-203 can induce the E-cadherin mRNA expression and inhibit N-cadherin and Vimentin mRNA expression using qRT-PCR. (B) Overexpression of miR-203 can induce the E-cadherin protein expression and inhibit N-cadherin and Vimentin protein expression using western blot. (C) The mRNA expression of Twist, Snail, Slug, and Zeb1 was analyzed by using qRT-PCR. **p<0.01 and ***p<0.001.</p

Poly(thymine)-Templated Selective Formation of Copper Nanoparticles for Alkaline Phosphatase Analysis Aided by Alkyne–Azide Cycloaddition “Click” Reaction

Alkaline phosphatase (ALP) is closely associated with many health disorders like liver diseases and bone diseases, which is a routine index of blood examination. In the current study, a fluorescent method for the detection of ALP is established based on poly­(thymine)-templated selective formation of copper nanoparticles (CuNPs) and alkyne–azide cycloaddition “click” reaction. Generally, in the presence of ALP, l-ascorbic acid-2-phosphate is hydrolyzed, and the generated ascorbic acid reduces Cu²⁺ to Cu⁺, which further catalyzes alkyne–azide cycloaddition click reaction between two poly­(thymine) segments. The two DNA fragments (18 thymine) are thus ligated, forming the DNA template (36 thymine), which is effective for the synthesis of CuNPs. Experimental results show that the fluorescence response of CuNPs increases with higher ALP concentration from 0.1 to 40 U/mL, and the limit of detection is as low as 0.05 U/mL. Furthermore, the proposed method is highly selective and is capable of detecting ALP in biological fluidlike serum, which suggests its wide biomedical applications

Hydration of Nitriles to Amides by Thiolate-Bridged Diiron Complexes

A series of nitrile-coordinating complexes [Cp*Fe­(μ-SEt)­RCN]₂[PF₆]₂ (<b>1</b>, R = alkyl, aryl, vinyl, amine) have been obtained by the reaction of [Cp*Fe­(μ-SEt)­MeCN]₂[PF₆]₂ (<b>1a</b>) with various nitriles in acetone. Complexes <b>1</b> can realize the hydration of a nitrile ligand under ambient conditions. Complexes [Cp*Fe­(μ-SEt)₂(μ-η¹:η¹-NH­(O)­CR)­FeCp*]­[PF₆] (<b>2</b>) were successfully isolated as intermediates during the hydration process, with <b>2b</b> and <b>2e</b> (R = CH₂CH and Et₂N) being characterized by spectrometry and X-ray crystallography. Treatment of <b>2</b> with HBF₄·Et₂O in the presence of nitriles released corresponding amides <b>3</b>. At the same time, the structural features of the [Fe₂S₂] scaffold were retained. These results confirmed that the hydration of nitriles was realized by cooperative interaction on diiron centers

Additional file 1 of Agreement and correlation of abdominal skeletal muscle area measured by CT and MR imaging in cirrhotic patients

Additional file 1. Table S1. The original data of the baseline clinical characteristics of the patients

MS2Analyzer: A Software for Small Molecule Substructure Annotations from Accurate Tandem Mass Spectra

Systematic analysis and interpretation of the large number of tandem mass spectra (MS/MS) obtained in metabolomics experiments is a bottleneck in discovery-driven research. MS/MS mass spectral libraries are small compared to all known small molecule structures and are often not freely available. MS2Analyzer was therefore developed to enable user-defined searches of thousands of spectra for mass spectral features such as neutral losses, <i>m</i>/<i>z</i> differences, and product and precursor ions from MS/MS spectra in MSP/MGF files. The software is freely available at http://fiehnlab.ucdavis.edu/projects/MS2Analyzer/. As the reference query set, 147 literature-reported neutral losses and their corresponding substructures were collected. This set was tested for accuracy of linking neutral loss analysis to substructure annotations using 19 329 accurate mass tandem mass spectra of structurally known compounds from the NIST11 MS/MS library. Validation studies showed that 92.1 ± 6.4% of 13 typical neutral losses such as acetylations, cysteine conjugates, or glycosylations are correct annotating the associated substructures, while the absence of mass spectra features does not necessarily imply the absence of such substructures. Use of this tool has been successfully demonstrated for complex lipids in microalgae

Additional file 1 of An optimized optical-flow-based method for quantitative tracking of ultrasound-guided right diaphragm deformation

Supplementary Material

Additional file 2 of An optimized optical-flow-based method for quantitative tracking of ultrasound-guided right diaphragm deformation

Supplementary Material

Annotated lipids in <i>C</i>. <i>reinhardtii</i> under nitrogen and sulfur stress conditions.

<p>^a: represented these lipids only can be annotated using Lipidblast;</p><p>^b: represented these lipids only can be annotated using MS2Analyzer;</p><p>^c: represented these lipids can be annotated by both databases.</p><p>The reverse dot product represents the level of confidence from in silico-MS/MS library search. Compound annotations without reverse dot product were annotated using MS2Analyzer.</p