Pappas_et_al_all data

Pappas_et_al_all dat

Local and systemic effects of plant exposure to <i>Macrolophus pygmaeus</i> on tomato proteinase inhibitor levels.

<p>Mean (+/- SE) of (A) proteinase inhibitor (PI) activity relative to protein content (N = 18/19 for control/exposed plants) and (B) transcript accumulation of <i>PI-I</i> and <i>PI-II</i> genes (N = 5/6 for control/exposed plants, each replicate representing 3 plants) in the local leaflet (Lf) exposed to 5 young <i>M</i>. <i>pygmaeus</i> females (dark bars) in clip cages for 4 days (depicted in inset i) and in unexposed control plants (white bars). Transcript accumulation expressed relative to control plants was assessed by real time PCR in relation to ubiquitin as reference gene. (C, D) PI activity relative to protein content (mean +/- SE, N = 10/9 for control/exposed plants) of a systemic leaf (L5, pooled Lf 2–4) either directly after (C: No delay) or 4 additional days after (D: Delay) a 4-day exposure of an older leaf (L2), to either 5 young adult females or 10 nymphs of <i>M</i>. <i>pygmaeus</i> (depicted in inset ii). Significant differences between exposed and control plants are indicated by asterisks following a GLM: n.s.: not significant, <i>P</i> < 0.05 (*), <i>P</i> < 0.01 (**).</p

Persistence of plant-mediated effects of <i>Macrolophus pygmaeus</i> on the performance of <i>Tetranychus urticae</i>.

<p>(A) Number of eggs per plant and (B) survival of females of <i>T</i>. <i>urticae</i> (mean +/- SE of 8 plants) 2 weeks after exposure of young plants to 2 <i>M</i>. <i>pygmaeus</i> females for 4 days (dark bars, setup is depicted in inset) or the corresponding control plants (white bars). Significant differences between exposed plants and control plants are indicated by asterisks following a GLMM: <i>P</i> < 0.05 (*), <i>P</i> < 0.001 (***).</p