Antimicrobial response

Data from lytic zone assay, where inhibition zones created by heamolymph was measured in (mm). PlateNumber describes the individual agar plate

Survival Males

Long-term survival males (used in proportional hazard model)

SurvivalFemales

Long-term survival females (used in proportional hazard model)

Sperm competition

Including data on sperm competition and on sperm competition success as binary data (yes or no)

EggtoAdult viability data

Number of eggs that hatch out to adults

Female fecundity data

Total number of eggs for each female, including individuals laying no eggs

Correlations Fitness Immunity

Overall means (n=5) of fitness and immune data used for supporting material

All data for Transgenerational Effects manuscript

All data for the manuscript in an Excel file. Details can be found in the first spreadsheet and ESM

Age-dependent change in sperm viability across treatment classes.

<p>Each panel (a–c) denotes sperm viability (LS mean ±1 standard error) of males at day six and 13 of the experiment; a = males assigned to sexually deprived class, b = males assigned to courtship class, c = males assigned to courtship and mating class. Sample sizes per group are indicated above the bars.</p

Supplementary Figures and tables from Mitochondrial genetic effects on reproductive success: signatures of positive intrasexual, but negative intersexual pleiotropy

Theory predicts that maternal inheritance of mitochondria will facilitate the accumulation of mtDNA mutations that are male biased, or even sexually antagonistic, in effect. While there are many reported cases of mtDNA mutations conferring cytoplasmic male sterility in plants, historically it was assumed such mutations would not persist in the streamlined mitochondrial genomes of bilaterian metazoans. Intriguingly, recent cases of mitochondrial variants exerting male biases in effect have come to light in bilaterians. These cases aside, it remains unknown whether the mitochondrial genetic variation affecting phenotypic expression, and in particular reproductive performance, in bilaterians is routinely composed of sex-biased or sex-specific variation. If selection consistently favours mtDNA variants that augment female fitness, but at cost to males, this could shape patterns of pleiotropy and lead to negative intersexual correlations across mtDNA haplotypes. Here, we show that genetic variation across naturally occurring mitochondrial haplotypes affects components of reproductive success in both sexes, in the fruit fly <i>Drosophila melanogaster</i>. We find that intrasexual correlations across mitochondrial haplotypes, for components of reproductive success, are generally positive, while intersexual correlations are negative. These results accord with theoretical predictions, suggesting that maternal inheritance has led to the fixation of numerous mutations of sexually antagonistic effect