120 research outputs found

    Estimating sharka dispersal function by stochastic spatiotemporal modelling

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    Plant viral diseases, and especially the ones transmitted by aerial vectors, can cause considerable yield losses. A good knowledge of the distances of spread is key to the understanding of disease dynamics. Exploratory approaches aiming at characterizing the spatiotemporal distribution of diseased plants are often used to get an insight into the distances of spread. A more powerful approach is based on stochastic spatiotemporal modelling in order to estimate the dispersal function of the disease (probability density function describing the probability for an infectious plant to infect a healthy plant at distance d). In this study, we implemented a method for estimating the dispersal function of the sharka disease. Sharka is one of the most serious diseases of stone fruit trees (Prunus sp.). It is caused by Plum pox virus (PPV, genus Potyvirus), transmitted by at least twenty different aphid species in a non persistent manner. Due to the inefficiency of insecticides and the very rare sources of resistance against the virus available in the host species, prophylactic disease control is based on the removal of the diseased trees in the orchards. Thus, a very good knowledge of the dispersal function of sharka is crucial for building epidemiological models and optimizing the strategies of surveillance and control. We adapted the methodology published by Gibson (1997) based on a Markov chain Monte Carlo (MCMC) algorithm in order to estimate sharka dispersal function from the maps of 157 contiguous peach orchards reporting the exact location and the sanitary status (asymptomatic/symptomatic) of each of the trees during six consecutive years. An estimation method based on the Gibbs sampling algorithm was developed taking into account the specificities of the dataset (more than two dates of observation, annual removal of diseased trees). This estimation algorithm was validated on simulated data and was proved to be more powerful and better adapted to large datasets than the one proposed by Gibson. Moreover, the influence of latency on the estimation of the dispersal function was quantified. This methodology was then used to estimate the dispersal function of the disease from a subset of the real dataset. The methods developed in this study are generic enough to be used and adapted for the estimation of dispersal functions of any disease transmitted in a non persistent manner, and even for diseases with similar characteristics. (Texte intégral

    Ecologie de la sharka en France : Caractérisation moléculaire et biologique d'isolats épidémiologiquement différenciés. Eléments pour des stratégies limitant la dissémination de la virose

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    *INRA, Centre de Montpellier (FRA) Diffusion du document : INRA, Centre de Montpellier (FRA) DiplĂ´me : Dr. Ing

    Epidemiology sf Sharka disease in France

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    International audiencePlum pox virus was first detected in France in the 1960s. Both PPV-D and PPV-M strains are present but epidemics related to the PPV-M strain detected in the late 1980s are the most problematic. The two PPV strains have unequal distributions in peach and apricot orchards and different prevalences. More than 20 different aphid species have been identified as vectors of PPV but most of them do not colonize Prunus species. Thus, aphids involved in the spread of PPV in orchards are essentially visiting aphids. The main sources of inoculum for the vectors are leaves and fruits of infected stone-fruit trees. Spontaneous, wild and ornamental Prunus species such as Prunus dulcis, P. spinosa or P. pissardii are susceptible to PPV isolates found in France but their role as a reservoir in sharka epidemics is probably negligible. The disease spreads rapidly in orchards but the rate of progression may vary according to the identity of the PPV strain and the Prunus species. Analysis of spatial patterns of disease has shown that secondary spread by aphids frequently occurs over short distances in the orchards (aggregated patterns) but also that dissemination at longer distances (of several hundred metres) is a common event

    Improved knowledge on key epidemiological parameters to optimize Sharka management strategies

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    Integrating genetic and epidemiological data to determine virus transmission pathways

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    First report and molecular characterization of YMMV on Dioscorea alata on the island of Martinique

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    umr bgpi equipe 6International audienceNaturally infected Dioscorea alata plants showing mild mosaic were collected in 1998 on the island of Martinique in the Caribbean. Isolates were first screened by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies raised against Yam mosaic virus (YMV) and antigen-coated plate ELISA with universal potyvirus monoclonal antibodies (Agdia, Elkhart, IN). A positive reaction was obtained only with the universal potyvirus antiserum. Immunocapture reverse-transcriptase polymerase chain reaction was performed for specific detection of Yam mild mosaic virus (YMMV [3]) and YMV. A product with the predicted size of 249 bp was obtained with YMMV primers. YMMV is a recently recognized distinct potyvirus infecting D. alata in West Africa and the South Pacific (2–4). It was originally described as Yam virus I and is synonymous with Dioscorea alata virus (4). To characterize the YMMV Martinique isolate, total RNA was extracted, and universal potyvirus degenerate primers (1) were used to amplify a 700-bp fragment that included the core and C-terminal region of the coat protein (CP) and 3' untranslated region (3'UTR). Sequence information generated (EMBL AJ250336) from the cloned fragment was compared with sequences of other yam potyviruses. Sequence comparisons of the partial CP (453 nt) showed a similarity of 94.6% (amino acids [aa]) with the YMMV isolate from Papua New Guinea (EMBL AB022424 [2]); 72.2% (aa) with the Japanese yam mosaic virus (JYMV) isolate (EMBL AB016500); and 67 to 73% (aa) with 27 YMV isolates. These sequences are most diverse in the 3'UTR, which showed a similarity of 72.8% with the YMMV Papua New Guinea isolate, 30% with the JYMV isolate, and 26% with the YMV isolates. These results confirm, as previously shown by S. Fuji et al. (2), that YMMV should be classified as a new potyvirus of yam. This is the first report of the natural occurrence of YMMV in the Caribbean

    The use of phylogenetic data to develop molecular tools for the detection and genotyping of Yam mosaic virus. Potential application in molecular epidemiology

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    UMR BGPI Equipe 6International audienceMolecular detection of Yam mosaic virus (YMV), the most important potyvirus for yam plants (Dioscorea spp.) has been limited by its high genetic diversity. According to recent phylogenetic data, suitable molecular tools were developed for detection and genotyping. A reliable and rapid molecular test is described using single-tube immunocapture (IC)-RT-PCR combining the use of a monoclonal antibody able to recognise all YMV serotypes and primers designed to take into account the whole variability of YMV. Phylogenetic data were then used to develop an uncoupled IC-RT-PCR procedure adapted to the genotyping of the three major phylogenetic groups and the two recombinants implicated in the YMV epidemics in the Caribbean and French Guiana. A third exploitation of phylogenetic data was carried out with the development of a typing test based on the analysis of short nucleotide sequences. The direct sequencing of a 450 nts fragment constituted a reliable complementary typing tool as the resulting information was quite similar to that obtained with sequences of the complete coat protein gene. The approach, consisting in a robust study of YMV variability followed by the use of phylogenetic data to develop reliable detection and genotyping tools, offers new perspectives for powerful molecular epidemiological studies
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