Home Range and Movement of Fox Squirrels in East Central Illinois

Home ranges and movements were determined for 11 fox squirrels (Sciurus niger) and analyzed with respect to the distribution of mast trees and den trees on a 12¼ acre wooded plot in east central Illinois. Average home range size for all squirrels was 0.9 acres. The home range of adult squirrels averaged 1.5 acres while juveniles averaged 0.8 acres. Females had larger home ranges (1.4 acres) than males (0.6 acres). The average distance moved within the home range was 360 feet for all squirrels. The average distances moved by adults and juveniles was nearly the same (275-280 feet), but females moved farther (340 feet) than males (170 feet). Home ranges were linear in shape. Linearity was greatest for juvenile females (1:2.7); greater in juveniles (1:2.2) than adults (1:1.4), and greater in females (1:1.8) than males (1:1.5). Seventy-eight percent of all available den trees and sixty-five percent of all available mast trees were located within the home ranges of the 11 observed squirrels

Home Range and Movement of Fox Squirrels in East Central Illinois

Home ranges and movements were determined for 11 fox squirrels (Sciurus niger) and analyzed with respect to the distribution of mast trees and den trees on a 12¼ acre wooded plot in east central Illinois. Average home range size for all squirrels was 0.9 acres. The home range of adult squirrels averaged 1.5 acres while juveniles averaged 0.8 acres. Females had larger home ranges (1.4 acres) than males (0.6 acres). The average distance moved within the home range was 360 feet for all squirrels. The average distances moved by adults and juveniles was nearly the same (275-280 feet), but females moved farther (340 feet) than males (170 feet). Home ranges were linear in shape. Linearity was greatest for juvenile females (1:2.7); greater in juveniles (1:2.2) than adults (1:1.4), and greater in females (1:1.8) than males (1:1.5). Seventy-eight percent of all available den trees and sixty-five percent of all available mast trees were located within the home ranges of the 11 observed squirrels

Novel approach to plasma facing materials in nuclear fusion reactors

A novel material design in nuclear fusion reactors is proposed based on W-nDiamond nanostructured composites. Generally, a microstructure refined to the nanometer scale improves the mechanical strength due to modification of plasticity mechanisms. Moreover, highly specific grainboundary area raises the number of sites for annihilation of radiation induced defects. However, the low thermal stability of fine-grained and nanostructured materials demands the presence of particles at the grain boundaries that can delay coarsening by a pinning effect. As a result, the concept of a composite is promising in the field of nanostructured materials. The hardness of diamond renders nanodiamond dispersions excellent reinforcing and stabilization candidates and, in addition, diamond has extremely high thermal conductivity. Consequently, W-nDiamond nanocomposites are promising candidates for thermally stable first-wall materials. The proposed design involves the production of WAV-nDiamondAV-Cu/Cu layered castellations. The W, W-nDiamond and W-Cu layers are produced by mechanical alloying followed by a consolidation route that combines hot rolling with spark plasma sintering (SPS). Layer welding is achieved by spark plasma sintering. The present work describes the mechanical alloying processsing and consolidation route used to produce W-nDiamond composites, as well as microstructural features and mechanical properties of the material produced Long term plasma exposure experiments are planned at ISTTOK and at FTU (Frascati)

A FreeSurfer-compliant consistent manual segmentation of infant brains spanning the 0-2 year age range

We present a detailed description of a set of FreeSurfer compatible segmentation guidelines tailored to infant MRI scans, and a unique data set of manually segmented acquisitions, with subjects nearly evenly distributed between 0 and 2 years of age. We believe that these segmentation guidelines and this dataset will have a wide range of potential uses in medicine and neuroscience.Eunice Kennedy Shriver National Institute of Child Health and Human Development (U.S.) (Grant 1K99HD061485-01A1)Eunice Kennedy Shriver National Institute of Child Health and Human Development (U.S.) (Grant R00 HD061485-03)Ralph Schlaeger FellowshipNational Institutes of Health (U.S.) (1R01EB014947-01)National Institutes of Health (U.S.) (K23 NS42758-01)National Center for Research Resources (U.S.) (P41-RR14075)National Center for Research Resources (U.S.) (U24 RR021382)National Institutes of Health. National Institute for Biomedical Imaging and Bioengineering (R01EB006758)National Institute on Aging (AG022381)National Institute on Aging (5R01AG008122-22)National Institute of Neurological Disorders and Stroke (U.S.) (R01 NS052585-01)National Institute of Neurological Disorders and Stroke (U.S.) (1R21NS072652-01)National Institute of Neurological Disorders and Stroke (U.S.) (1R01NS070963)National Center for Research Resources (U.S.) (Shared Instrumentation Grant 1S10RR023401)National Center for Research Resources (U.S.) (Shared Instrumentation Grant 1S10RR019307)National Center for Research Resources (U.S.) (Shared Instrumentation Grant 1S10RR023043)Ellison Medical FoundationNational Institutes of Health. Blueprint for Neuroscience Research (5U01-MH093765)Human Connectome Projec

Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII a in epileptic hippocampal neurons

Purpose: To investigate the mechanism of the anti-epileptic effect of Ganoderma lucidum polysaccharides (GLP), the changes of intracellular calcium and CaMK II a expression in a model of epileptic neurons were investigated. Method: Primary hippocampal neurons were divided into: 1) Control group, neurons were cultured with Neurobasal medium, for 3 hours; 2) Model group I: neurons were incubated with Mg2+ free medium for 3 hours; 3) Model group II: neurons were incubated with Mg2+ free medium for 3 hours then cultured with the normal medium for a further 3 hours; 4) GLP group I: neurons were incubated with Mg2+ free medium containing GLP (0.375 mg/ml) for 3 hours; 5) GLP group II: neurons were incubated with Mg2+ free medium for 3 hours then cultured with a normal culture medium containing GLP for a further 3 hours. The CaMK II a protein expression was assessed by Western-blot. Ca2+ turnover in neurons was assessed using Fluo-3/AM which was added into the replacement medium and Ca2+ turnover was observed under a laser scanning confocal microscope. Results: The CaMK II a expression in the model groups was less than in the control groups, however, in the GLP groups, it was higher than that observed in the model group. Ca2+ fluorescence intensity in GLP group I was significantly lower than that in model group I after 30 seconds, while in GLP group II, it was reduced significantly compared to model group II after 5 minutes. Conclusion: GLP may inhibit calcium overload and promote CaMK II a expression to protect epileptic neuron

Tissue invasion and metastasis: molecular, biological and clinical perspectives

Cancer is a key health issue across the world, causing substantial patient morbidity and mortality. Patient prognosis is tightly linked with metastatic dissemination of the disease to distant sites, with metastatic diseases accounting for a vast percentage of cancer patient mortality. While advances in this area have been made, the process of cancer metastasis and the factors governing cancer spread and establishment at secondary locations is still poorly understood. The current article summarizes recent progress in this area of research, both in the understanding of the underlying biological processes and in the therapeutic strategies for the management of metastasis. This review lists the disruption of E-cadherin and tight junctions, key signaling pathways, including urokinase type plasminogen activator (uPA), phosphatidylinositol 3-kinase/v-akt murine thymoma viral oncogene (PI3K/AKT), focal adhesion kinase (FAK), β-catenin/zinc finger E-box binding homeobox 1 (ZEB-1) and transforming growth factor beta (TGF-β), together with inactivation of activator protein-1 (AP-1) and suppression of matrix metalloproteinase-9 (MMP-9) activity as key targets and the use of phytochemicals, or natural products, such as those from Agaricus blazei, Albatrellus confluens, Cordyceps militaris, Ganoderma lucidum, Poria cocos and Silybum marianum, together with diet derived fatty acids gamma linolenic acid (GLA) and eicosapentanoic acid (EPA) and inhibitory compounds as useful approaches to target tissue invasion and metastasis as well as other hallmark areas of cancer. Together, these strategies could represent new, inexpensive, low toxicity strategies to aid in the management of cancer metastasis as well as having holistic effects against other cancer hallmarks.W.G. Jiang ... S.K. Thompson ... et al

Intervention effects of Ganoderma lucidum spores on epileptiform discharge hippocampal neurons and expression of Neurotrophin-4 and N-Cadherin

Epilepsy can cause cerebral transient dysfunctions. Ganoderma lucidum spores (GLS), a traditional Chinese medicinal herb, has shown some antiepileptic effects in our previous studies. This was the first study of the effects of GLS on cultured primary hippocampal neurons, treated with Mg2+ free medium. This in vitro model of epileptiform discharge hippocampal neurons allowed us to investigate the anti-epileptic effects and mechanism of GLS activity. Primary hippocampal neurons from <1 day old rats were cultured and their morphologies observed under fluorescence microscope. Neurons were confirmed by immunofluorescent staining of neuron specific enolase (NSE). Sterile method for GLS generation was investigated and serial dilutions of GLS were used to test the maximum non-toxic concentration of GLS on hippocampal neurons. The optimized concentration of GLS of 0.122 mg/ml was identified and used for subsequent analysis. Using the in vitro model, hippocampal neurons were divided into 4 groups for subsequent treatment i) control, ii) model (incubated with Mg2+ free medium for 3 hours), iii) GLS group I (incubated with Mg2+ free medium containing GLS for 3 hours and replaced with normal medium and incubated for 6 hours) and iv) GLS group II (neurons incubated with Mg2+ free medium for 3 hours then replaced with a normal medium containing GLS for 6 hours). Neurotrophin-4 and N-Cadherin protein expression were detected using Western blot. The results showed that the number of normal hippocampal neurons increased and the morphologies of hippocampal neurons were well preserved after GLS treatment. Furthermore, the expression of neurotrophin-4 was significantly increased while the expression of N-Cadherin was decreased in the GLS treated group compared with the model group. This data indicates that GLS may protect hippocampal neurons by promoting neurotrophin-4 expression and inhibiting N-Cadherin expression

Development of Entrepreneurial Attitudes Assessment Instrument for Freshman Students

An increasing population of university programs and quantity of curricular content focused on entrepreneurship poses both enormous opportunities for student growth, and numerous practical challenges. Prior work has largely focused on pre-post assessment of student learning, shifts in‘mindset’, activity effectiveness, mapping of student outcomes, and implications of student learning on career success. A baseline of freshman student attitudes towards entrepreneurship,outside of specifically focused entrepreneurial leaning, has significant potential to identify and inform programming in entrepreneurship, as well as general curriculums and pedagogy. An improved understanding of student’s constructive and cognitive influences in entrepreneurial education will serve to better inform the way entrepreneurship education in engineering is historically and currently discussed. Improving entrepreneurship education models begins with understanding student backgrounds comprised of different experiences, knowledge, and preconceptions. When looking longitudinally, migratory information can better inform entrepreneurial programming to provide data and support for more organized and integrated approaches to entrepreneurship education.This work in progress provides initial results and validation on the quantitative instrument portion of a mixed methods study developed for assessing and tracking entrepreneurial behaviors, experiences, and attitudes in a way identifiable to engineering and business freshman.The instrument is modified from the Entrepreneurial Attitude Orientation (EAO) survey instrument developed by Robbins in the early 1990s. Additionally, a section for gathering student socioeconomic status and gender, using elements of the Academic Pathways of People Learning Engineering Survey (APPLES) is included. Based on the difficulties in operationalization of student socioeconomic status self-identification, normalizing question are added as suggested by Donaldson and Sheppard from results in the APPLES instrument development process.The quantitative portion, discussed here, survey will be deployed during winter of 2015, to business and engineering freshman at a large Midwestern university. The initial deployment will allow validation against prior uses of the EAO instrument. Results will be presented in the paper and conference presentation comparing gender and socioeconomic correlations to entrepreneurial attitudes with previous publications. The survey instrument is being developed as the quantitative element of a mixed method longitudinal study tracking student entrepreneurial attitudes, focus, and growth over student college experiences. Follow-on efforts are intended to help better inform educators about the nature of student construction and growth in an university space increasingly influenced by a move towards entrepreneurship education

Optimizing University Mobility : An Internal Navigation and Crowd Management System

In the evolving landscape of educational technology, the article explores the critical frontier of indoor navigation systems, focusing on universities. Traditional approaches in higher education often fall short of meeting dynamic user expectations, necessitating revolutionary solutions. This research introduces an innovative internal navigation and crowd management system that seamlessly integrates augmented reality, natural language processing, machine learning, and image processing technologies. The Android platform serves as the foundation, harnessing augmented reality's transformative capabilities to provide real-time visual cues and personalized wayfinding experiences. The voice interaction module, backed by NLP and ML, creates an intelligent, context-aware assistant. The crowd management module, employing advanced image processing, delivers real-time crowd density insights. Personalized recommendations, powered by NLP and ML, offer tailored canteen suggestions based on user preferences. The agmented reality navigation module, using Mapbox, Unity Hub, AR Core, and Vuforia, enriches the user experience with dynamic visual cues. Results reveal the success of each module: the voice interaction module showcases continuous learning, user-centric feedback, contextual guidance excellence, robust security, and multimodal interaction flexibility. The crowd management module excels in video feed processing, image processing with OpenCV, and real-time availability information retrieval. The personalized recommendations module demonstrates high accuracy, equilibrium, and robust performance. The AR navigation module impresses with precision, enriched navigation, and tailored routes through machine learning. This cohesive system sets new benchmarks for user-centric technology in universities. Future work includes multi-university integration, intelligent spatial design, and real-time decision support, paving the way for more efficient, user-centered university experiences and contributing to the advancement of smart university environments. The research serves as a pivotal force in reshaping interactions within university spaces, envisioning a future where technology seamlessly enhances the essence of human interaction in educational environments

Designing a broad-spectrum integrative approach for cancer prevention and treatment

Targeted therapies and the consequent adoption of "personalized" oncology have achieved notablesuccesses in some cancers; however, significant problems remain with this approach. Many targetedtherapies are highly toxic, costs are extremely high, and most patients experience relapse after a fewdisease-free months. Relapses arise from genetic heterogeneity in tumors, which harbor therapy-resistantimmortalized cells that have adopted alternate and compensatory pathways (i.e., pathways that are notreliant upon the same mechanisms as those which have been targeted). To address these limitations, aninternational task force of 180 scientists was assembled to explore the concept of a low-toxicity "broad-spectrum" therapeutic approach that could simultaneously target many key pathways and mechanisms. Using cancer hallmark phenotypes and the tumor microenvironment to account for the various aspectsof relevant cancer biology, interdisciplinary teams reviewed each hallmark area and nominated a widerange of high-priority targets (74 in total) that could be modified to improve patient outcomes. For thesetargets, corresponding low-toxicity therapeutic approaches were then suggested, many of which werephytochemicals. Proposed actions on each target and all of the approaches were further reviewed forknown effects on other hallmark areas and the tumor microenvironment. Potential contrary or procar-cinogenic effects were found for 3.9% of the relationships between targets and hallmarks, and mixedevidence of complementary and contrary relationships was found for 7.1%. Approximately 67% of therelationships revealed potentially complementary effects, and the remainder had no known relationship. Among the approaches, 1.1% had contrary, 2.8% had mixed and 62.1% had complementary relationships. These results suggest that a broad-spectrum approach should be feasible from a safety standpoint. Thisnovel approach has potential to be relatively inexpensive, it should help us address stages and types ofcancer that lack conventional treatment, and it may reduce relapse risks. A proposed agenda for futureresearch is offered