Attentive Computing

Non

The effect of storage time and freeze-thaw cycles on the stability of serum samples

Introduction: Optimal storage of serum specimens in central laboratories for a long period for multicenter reference interval studies, or epidemiologic studies remains to be determined. We aimed to examine the analytical stability of chemistry analytes following numerous freeze-thaw and long term storage. Materials and methods: Serum samples were obtained from 15 patients. Following baseline measurement, sera of each subject were aliquoted and stored at -20 ˚C for two experiments. A group of sera were kept frozen for up to 1, 2 and 3 months and then analyzed for stability. The other experiment consisted of one to ten times of freeze and thaw cycles. Total of 17 chemistry analytes were assayed at each time point. The results were compared with those obtained from the initial analysis of fresh samples. Median or mean changes from baseline (T0) concentrations were evaluated both statistically and clinically according to the desirable bias. Results: Of the analytes studied, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), gamma-glutamyl transferase (GGT), direct bilirubin, glucose, creatinine, cholesterol, triglycerides, high density lipoprotein (HDL) were stable in all conditions. Blood urea nitrogen (BUN), uric acid, total protein, albumin, total bilirubin, calcium, lactate dehydrogenase (LD) were changed significantly (P < 0.005). Conclusions: As a result, common clinical chemistry analytes, with considering the variability of unstable analytes, showed adequote stability after 3 months of storage in sera at -20 °C, or up to ten times of freeze-thaw cycle. All the same, such analysis can only be performed for exceptional cases, and this should be taken into account while planning studies

Optical cohorence tomography analysis of hollenhorst plaque

Retinal arter dal tıkanıklığı (RADT) sıklıkla bir emboliden kaynaklanan ani, ağrısız, görme alanı kaybı ile seyreden klinik tablodur. Hollenhorst Plağı (HP) kolesterol embolisinin neden olduğu, parlak sarı refraktil arteriyolar birikimdir. Bu çalışmada kliniğimizde fundus muayene bulguları ile RADT tanısı konulan 60 yaşında erkek hastanın, RADT ve Hollenhorst Plağı ile uyumlu optik koherens tomografi (OKT) bulguları sunulmaktadır

Association of serum and follicular fluid leptin and ghrelin levels with in vitro fertilization success

 Objectives: The aim of this study was to evaluate the relationship between in vitro fertilization (IVF) cycle outcomes, serum and follicular fluid (FF) levels of leptin and ghrelin. Material and methods: Forty-four women who underwent intracytoplasmic sperm injection cycles (ICSI) were enrolled in the study. On the third day (D3) of the menstrual cycle, venous blood samples were drawn for serum measurements of leptin and ghrelin. The follicular fluid (FF) and the corresponding oocyte were obtained from a single dominant preovulatory follicle at the time of oocyte pick-up. The FF and D3 serum leptin and ghrelin concentrations were measured by enzyme-linked immunosorbent assay. The relationship between pregnancy rate and serum, follicular fluid levels of leptin and ghrelin were analyzed. Results: Of the 44 cases included, nineteen achieved clinical pregnancy (43.18%). Follicular fluid ghrelin levels were significantly lower in the pregnant group than non-pregnant group (p &lt; 0.05) With respect to FF leptin, there was no statistically significant differences between the pregnant and non-pregnant women (p &gt; 0.05). There was no statistically significant difference in D3 serum ghrelin between pregnant and non-pregnant groups (p &gt; 0.05). However, D3 serum leptin levels were significantly lower in pregnant women than non-pregnant women (p &lt; 0.05). Conclusions: Lower ghrelin levels in the follicular fluid were associated with higher pregnancy rates. Also, D3 serum leptin levels were inversely correlated with clinical pregnancy rates. These findings support the potential role of these molecules on IVF outcomes

HASTALAR İÇİN KEMOTERAPİ EL KİTABI

Onkoloji Hastalarına uygulanan kemoterapi hayati bir tedavidir.Bu kitap hastalarımıza yönelik bir el kitapıdır.Kemoterapi konusunda karşılaşılacak sorunları aydınlatmayı amaçlamaktadır.</p

Development and validation of the stigma scale for epilepsy in Turkey

Objective: Epilepsy is a chronic disease with an increased risk of stigma. The aim of this study was to investigate the efficacy of a scale developed by the authors to determine the level of stigma in Turkish patients with epilepsy and their relatives

Long-term results of graded pneumatic dilatation under endoscopic guidance in patients with primary esophageal achalasia

AIM: Achalasia is the best known primary motor disorder of the esophagus in which the lower esophageal sphincter (LES) has abnormally high resting pressure and incomplete relaxation with swallowing. Pneumatic dilatation remains the first choice of treatment. The aims of this study were to determine the long term clinical outcome of treating achalasia initially with pneumatic dilatation and usefulness of pneumatic dilatation technique under endoscopic observation without fluoroscopy