5 research outputs found
Impact of the COVID-19 pandemic crisis on the health care system in less developed regions of Poland
PURPOSE: The aim of the study was to develop a model for assessing the satisfaction of
patients of GP practice with access to health services in the Lubelskie Voivodeship, in terms
of eliminating inequalities in the quality of these services and developing a scenario for the
revitalization of the health care system in relation to the inhabitants of the underdeveloped
region in the period after the cessation of pandemic.DESIGN/METHODOLOGY/APPROACH: The research method included developing a research tool
in the form of a survey questionnaire, validating the tool, conducting Phase I and II of the
survey, and evaluating the performance of the outpatient specialty care subsystem. The
analysis of the research results was directed at developing the assumptions of a model for
restoring functionality and increasing resilience of the healthcare system.FINDINGS: It was found that the level of patient satisfaction with the functioning of the health
care system during a pandemic, both in the form of medical tele-consultation and in the form
of direct contact with personnel in a health care facility, depends on the age and the level of
education. There were no differences in terms of sex and place of residence (town, village). It
was also found that the very limited availability of advanced diagnostic methods, showing an
unfavorable trend in the period preceding the outbreak of the pandemic, deteriorated further
and poses a critical threat to the functioning of the health care system for patients with
chronic diseases (oncological, neurodegenerative etc.)PRACTICAL IMPLICATIONS: The results of the research will be used to construct a model for the
reconstruction and revitalization of the health care system in the Lubelskie Voivodeship
after the pandemic.ORIGINALITY/VALUE: The study presents results of the current research. The development of
the research results will be continued in order to construct a comprehensive model.This article in part on basic healthcare presents the results of research
funded under the Faculty of Management of the Lublin University of Technology
(Discipline Council for Management and Quality Sciences) 2020/2021.peer-reviewe
Expression Analysis of PIN Genes in Root Tips and Nodules of Lotus japonicus
Auxins are postulated to be one of the pivotal factors in nodulation. However, their transporters in Lotus japonicus, the model species for the study of the development of determinate-type root nodules, have been scarcely described so far, and thus their role in nodulation has remained unknown. Our research is the first focusing on polar auxin transporters in L. japonicus. We analyzed and compared expression of PINs in 20 days post rhizobial inoculation (dpi) and 54 dpi root nodules of L. japonicus by real-time quantitative polymerase chain reaction (qPCR) along with the histochemical β-glucuronidase (GUS) reporter gene assay in transgenic hairy roots. The results indicate that LjPINs are essential during root nodule development since they are predominantly expressed in the primordia and young, developing nodules. However, along with differentiation, expression levels of several PINs decreased and occurred particularly in the nodule vascular bundles, especially in connection with the root’s stele. Moreover, our study demonstrated the importance of both polar auxin transport and auxin intracellular homeostasis during L. japonicus root nodule development and differentiation