Scaling K2. I. Revised Parameters for 222,088 K2 Stars and a K2 Planet Radius Valley at 1.9 R_⊕

Previous measurements of stellar properties for K2 stars in the Ecliptic Plane Input Catalog largely relied on photometry and proper motion measurements, with some added information from available spectra and parallaxes. Combining Gaia DR2 distances with spectroscopic measurements of effective temperatures, surface gravities, and metallicities from the Large Sky Area Multi-Object Fibre Spectroscopic Telescope (LAMOST) DR5, we computed updated stellar radii and masses for 26,838 K2 stars. For 195,250 targets without a LAMOST spectrum, we derived stellar parameters using random forest regression on photometric colors trained on the LAMOST sample. In total, we measured spectral types, effective temperatures, surface gravities, metallicities, radii, and masses for 222,088 A, F, G, K, and M-type K2 stars. With these new stellar radii, we performed a simple reanalysis of 299 confirmed and 517 candidate K2 planet radii from Campaigns 1–13, elucidating a distinct planet radius valley around 1.9 R_⊕, a feature thus far only conclusively identified with Kepler planets, and tentatively identified with K2 planets. These updated stellar parameters are a crucial step in the process toward computing K2 planet occurrence rates

PI3K Enters Beta-Testing

Phosphoinositide-3-OH kinases (PI3K) are critical regulators of cell metabolism, growth, and survival. In a recent publication in Nature, Jia et al. (2008) identify specific functions of the p110β isoform of PI3K in glucose metabolism, cellular proliferation, and tumorigenesis

A randomized trial of a lab-embedded discourse intervention to improve research ethics.

We report a randomized trial of a research ethics training intervention designed to enhance ethics communication in university science and engineering laboratories, focusing specifically on authorship and data management. The intervention is a project-based research ethics curriculum that was designed to enhance the ability of science and engineering research laboratory members to engage in reason giving and interpersonal communication necessary for ethical practice. The randomized trial was fielded in active faculty-led laboratories at two US research-intensive institutions. Here, we show that laboratory members perceived improvements in the quality of discourse on research ethics within their laboratories and enhanced awareness of the relevance and reasons for that discourse for their work as measured by a survey administered over 4 mo after the intervention. This training represents a paradigm shift compared with more typical module-based or classroom ethics instruction that is divorced from the everyday workflow and practices within laboratories and is designed to cultivate a campus culture of ethical science and engineering research in the very work settings where laboratory members interact

Determining a minimum detection threshold in terminal restriction fragment length polymorphism analysis

Terminal restriction fragment length polymorphism (T-RFLP) analysis is a common technique used to characterize soil microbial diversity. The fidelity of this technique in accurately reporting diversity has not been thoroughly evaluated. Here we determine if rare fungal species can be reliably detected by T-RFLP analysis. Spores from three arbuscular mycorrhizal fungal species were each mixed at a range of concentrations (1%, 10%, 50%, and 100%) with Glomus irregulare to establish a minimum detection threshold. T-RFLP analysis was capable of detecting diagnostic peaks of rare taxa at concentrations as low as 1%. The relative proportion of the target taxa in the sample and DNA concentration influenced peak detection reliability. However, low concentrations produced small, inconsistent electropherogram peaks contributing to difficulty in differentiating true peaks from signal noise. The results of this experiment suggest T-RFLP is a reproducible and high fidelity procedure, which requires careful data interpretation in order to accurately characterize sample diversity

Mesocorticolimbic monoamine correlates of methamphetamine sensitization and motivation.

Methamphetamine (MA) is a highly addictive psychomotor stimulant, with life-time prevalence rates of abuse ranging from 5-10% world-wide. Yet, a paucity of research exists regarding MA addiction vulnerability/resiliency and neurobiological mediators of the transition to addiction that might occur upon repeated low-dose MA exposure, more characteristic of early drug use. As stimulant-elicited neuroplasticity within dopamine neurons innervating the nucleus accumbens (NAC) and prefrontal cortex (PFC) is theorized as central for addiction-related behavioral anomalies, we used a multi-disciplinary research approach in mice to examine the interactions between sub-toxic MA dosing, motivation for MA and mesocorticolimbic monoamines. Biochemical studies of C57BL/6J (B6) mice revealed short- (1 day), as well as longer-term (21 days), changes in extracellular dopamine, DAT and/or D2 receptors during withdrawal from 10, once daily, 2 mg/kg MA injections. Follow-up biochemical studies conducted in mice selectively bred for high vs. low MA drinking (respectively, MAHDR vs. MALDR mice), provided novel support for anomalies in mesocorticolimbic dopamine as a correlate of genetic vulnerability to high MA intake. Finally, neuropharmacological targeting of NAC dopamine in MA-treated B6 mice demonstrated a bi-directional regulation of MA-induced place-conditioning. These results extend extant literature for MA neurotoxicity by demonstrating that even subchronic exposure to relatively low MA doses are sufficient to elicit relatively long-lasting changes in mesocorticolimbic dopamine and that drug-induced or idiopathic anomalies in mesocorticolimbic dopamine may underpin vulnerability/resiliency to MA addiction

The Abl interactor proteins localize to sites of actin polymerization at the tips of lamellipodia and filopodia

AbstractCell movement is mediated by the protrusion of cytoplasm in the form of sheet- and rod-like extensions, termed lamellipodia and filopodia. Protrusion is driven by actin polymerization, a process that is regulated by signaling complexes that are, as yet, poorly defined. Since actin assembly is controlled at the tips of lamellipodia and filopodia [1], these juxtamembrane sites are likely to harbor the protein complexes that control actin polymerization dynamics underlying cell motility. An understanding of the regulation of protrusion therefore requires the characterization of the molecular components recruited to these sites. The Abl interactor (Abi) proteins, targets of Abl tyrosine kinases [2–4], have been implicated in Rac-dependent cytoskeletal reorganization in response to growth factor stimulation [5]. Here, we describe the unique localization of Abi proteins in living, motile cells. We show that Abi-1 and Abi-2b fused to enhanced yellow fluorescent protein (EYFP) are recruited to the tips of lamellipodia and filopodia. We identify the targeting domain as the homologous N terminus of these two proteins. Our findings are the first to suggest a direct involvement of members of the Abi protein family in the control of actin polymerization in protrusion events, and establish the Abi proteins as potential regulators of motility

Scaling K2. II. Assembly of a Fully Automated C5 Planet Candidate Catalog Using EDI-Vetter

We present a uniform transiting exoplanet candidate list for Campaign 5 of the K2 mission. This catalog contains 75 planets with seven multi-planet systems (five double, one triple, and one quadruple planet system). Within the range of our search, we find eight previously undetected candidates, with the remaining 67 candidates overlapping 51% of the study of Kruse et al. that manually vets candidates from Campaign 5. In order to vet our potential transit signals, we introduce the Exoplanet Detection Identification Vetter (EDI-Vetter), which is a fully automated program able to determine whether a transit signal should be labeled as a false positive or a planet candidate. This automation allows us to create a statistically uniform catalog, ideal for measurements of planet occurrence rate. When tested, the vetting software is able to ensure that our sample is 94.2% reliable against systematic false positives. Additionally, we inject artificial transits at the light-curve level of the raw K2 data and find that the maximum completeness of our pipeline is 70% before vetting and 60% after vetting. For convenience of future studies of occurrence rate, we include measurements of stellar noise (CDPP) and the three-transit window function for each target. This study is part of a larger survey of the K2 data set and the methodology that will be applied to the entirety of that set

Scaling K2. III. Comparable Planet Occurrence in the FGK Samples of Campaign 5 and Kepler

Using our K2 Campaign 5 fully automated planet-detection data set (43 planets), which has corresponding measures of completeness and reliability, we infer an underlying planet population model for the FGK dwarf sample (9257 stars). Implementing a broken power law for both the period and radius distributions, we find an overall planet occurrence of 1.00^(+1.07)_(−0.51) planets per star within a period range of 0.5–38 days. Making similar cuts and running a comparable analysis on the Kepler sample (2318 planets; 94,222 stars), we find an overall occurrence of 1.10 ± 0.05 planets per star. Since the Campaign 5 field is nearly 120 angular degrees away from the Kepler field, this occurrence similarity offers evidence that the Kepler sample may provide a good baseline for Galactic inferences. Furthermore, the Kepler stellar sample is metal-rich compared to the K2 Campaign 5 sample, so a finding of occurrence parity may reduce the role of metallicity in planet formation. However, a weak (1.5σ) difference, in agreement with metal-driven formation, is found when assuming the Kepler model power laws for the K2 Campaign 5 sample and optimizing only the planet occurrence factor. This weak trend indicates that further investigation of metallicity-dependent occurrence is warranted once a larger sample of uniformly vetted K2 planet candidates is made available