6 research outputs found
The immunogenicity of a foot-and-mouth disease virus serotype O vaccine in commercial and subsistence cattle herds in Zambia
DATA AVAILABILITY STATEMENT : All datasets produced and analysed for this study are available from
the corresponding author upon reasonable request.SUPPLEMENTARY MATERIALS : TABLE S1: The solid-phase competitive ELISA (SPCE) % inhibition results at 1:10 dilution (results for 1:30 dilution not shown) and the log reciprocal of virus neutralisation test (VNT) titres in subsistence (Rufunsa) and commercial (Chisamba) cattle herds vaccinated with either one (day 0) or two doses (day 0 and 28) of an FMDV serotype O vaccine.The recent introduction of foot-and-mouth disease (FMD) virus serotype O (O/EA-2
topotype) in Southern Africa has changed the epidemiology of the disease and vaccine requirements
of the region. Commercial and subsistence cattle herds in Zambia were vaccinated with an FMD virus
serotype O Manisa vaccine according to a double- or single-dose vaccination schedule. Heterologous
antibody responses induced by this vaccine against a representative O/EA-2 virus from Zambia were
determined. Virus neutralisation tests (VNTs) showed double-dosed cattle had a mean reciprocal
log virus neutralisation titre of 2.02 (standard error [SE] = 0.16, n = 9) for commercial herds and 1.65
(SE = 0.17, n = 5) for subsistence herds 56 days after the first vaccination (dpv). Significantly lower
mean titres were observed for single-dosed commercial herds (0.90, SE = 0.08, n = 9) and subsistence
herds (1.15, SE = 0.18, n = 3) 56 dpv. A comparison of these results and those generated by solid-phase
competitive ELISA (SPCE) tests showed a statistically significant positive correlation by Cohen’s
kappa coefficient. Therefore, SPCE might be used in assessing the immunogenicity of vaccines
in place of VNT. Furthermore, for this vaccine and field strain, a vaccination regime employing a
two-dose primary course and revaccination after 4–6 months is likely to be appropriate.The Government of Zambia through the Ministry of Fisheries and Livestock, Department of Veterinary; the Department for Environment, Food, and Rural Affairs (Defra; UK), funded by the European Union; and The Pirbright Institute receives grant-aided support from the Biotechnology and Biological Sciences Research Council of the United Kingdom.https://www.mdpi.com/journal/vaccinesam2024Veterinary Tropical DiseasesSDG-02:Zero HungerSDG-03:Good heatlh and well-bein
Rapid, sensitive and effective diagnostic tools for foot-and-mouth disease virus in Africa
Speed is paramount in the diagnosis of highly infectious diseases, such as foot-and-mouth disease (FMD), as well as for emerging diseases; however, simplicity is required if a test is to be deployed in the field. Recent developments in molecular biology have enabled the specific detection of FMD virus (FMDV) by reverse-transcription loop-mediated isothermal amplification (RT-LAMP), real-time reverse-transcription polymerase chain reaction (RT-qPCR) and sequencing. RT-LAMP enables amplification of the FMDV RNA-dependent RNA polymerase 3D(pol) gene at 63 °C (in the presence of a primer mixture and both reverse transcriptase and Bst DNA polymerase) for 1 h, whilst RT-qPCR amplifies the same gene in approximately 2 h 30 min. In this study, we compared the sensitivity and effectiveness of RT-LAMP against RT-qPCR for the detection of the FMDV 3D(pol) gene in 179 oesophageal-pharyngeal scraping samples (collected by probang) obtained from clinically healthy cattle and buffalo in Malawi, Mozambique and Tanzania in 2010. The FMDV detection rate was higher with RT-LAMP (30.2%; n = 54) than with RT-qPCR (17.3%; n = 31). All samples positive by RT-qPCR (Cq ≤ 32.0) were also positive for the RT-LAMP assay; and both assays proved to be highly specific for the FMDV target sequence. In addition, the VP1 sequences of 10 viruses isolated from positive samples corresponded to the respective FMDV serotypes and genotypes. Our findings indicate that the performance of RT-LAMP is superior to RT-qPCR. Accordingly, we consider this test to have great potential with regard to the specific detection and surveillance of infectious diseases of humans and animals in resource-compromised developing countries