Christopher J. Kasanga

Cornelius Mundia

Donald P. King

Ezekia Ranga

Gaothlele Thobokwe

Jemma Wadsworth

Jimis Deve

Laureta Joao

Mark M. Rweyemamu

Misheck Mulumba

Mmeta Yongolo

Nick J. Knowles

Patrick Chikungwa

Philemon N. Wambura

Rahana Dwarka

Raphael Sallu

Onderstepoort Journal of Veterinary Research

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Abstractsdoi:10.4102/ojvr.v81i2.728http://www.ojvr.orgAuthors:Christopher J. Kasanga1Rahana Dwarka2Gaothlele Thobokwe3Jemma Wadsworth4Nick J. Knowles4Misheck Mulumba5Ezekia Ranga6Jimis Deve11Cornelius Mundia10Patrick Chikungwa8Laureta Joao9Raphael Sallu7Mmeta Yongolo7Philemon N. Wambura1Mark M. Rweyemamu1 Donald P. King4Affiliations:1Southern African Centre for Infectious Diseases Surveillance, Sokoine University of Agriculture, Tanzania2Onderstepoort Veterinary Institute, University of Pretoria, South Africa3Botswana Vaccine Institute, Botswana4WRLFMD, The Pirbright Institute, United Kingdom5Southern African Development Community Secretariat, Botswana6Ministry of Livestock Development and Fisheries, Tanzania7Tanzania Veterinary Laboratory Agency, Tanzania8Southern African Development Community, Transboundary Animal Diseases Section, Malawi 9Southern African Development Community, Transboundary Animal Diseases Section, Angola10Southern African Development Community, Transboundary Animal Diseases Section, Zambia11Southern African Development Community, Transboundary Animal Diseases Section, MozambiqueFoot-and-mouth disease (FMD) is endemic in most countries in southern Africa. African buffaloes (Syncerus caffer) are known to play a significant role in the transmission and dynamics of FMD virus (FMDV) in wildlife-livestock interface areas. The aim of this study was to investigate the serotype and determine the genetic relationships of FMDV recovered from animals in Tanzania, Zambia and Mozambique, and compare them with viruses detected from elsewhere in the sub-Saharan region. A total of 150 oesophageal-pharyngeal (probang) samples collected in 2010 from cattle and buffaloes in Marromeu (Mozambique), Katavi (Tanzania) and Lochninvar (Zambia) National Parks were used in this study. The presence of FMDV was determined by laboratory methods such as VI, antigen ELISA and real-time RT-PCR. Phylogenies of VP1 sequences were determined by the neighbour-joining method. The overall FMDV genome detection rate was 6.7% (n = 10), with SAT 1 being the most frequent serotype (60%; n = 6) isolated in cattle and buffaloes in Mozambique, Tanzania and Zambia followed by SAT 3 (30%; n = 3) and SAT 2 (10%; n = 1). Genotyping showed that type SAT 1 viruses fell into either the TOPOTYPE 1 (NWZ) or UNASSIGNED topotypes, type SAT 2 into the AFRICA topotype I and type SAT 3s into topotype IV (SEZ). This study reveals that serotypes SAT 1–3 are maintained in cattle and buffaloes in livestock-wildlife interface areas in Marromeu, Katavi, and Lochinvar National Parks. Phylogenetic analysis of FMDV isolates from Tanzania, Zambia and Mozambique showed that they are genetically related to lineages and topotypes from Africa. This information contributes to the understanding of the epidemiology of FMD in southern Africa. In Mozambique, Tanzania and Zambia, lack of consistent surveillance systems and animal movement controls make it difficult to determine the exact source of FMD and transmission dynamics of FMDV. Further studies are needed to elucidate the complex epidemiology of FMD in Africa.  AcknowledgementsThis work was supported by the Wellcome Trust Grant WT087546MA to the Southern African Centre for Infectious Diseases & Surveillance (SACIDS).Page 1 of 1Scan this QR code with your smart phone or mobile device to read online.Read online:Molecular biological characteristics of foot-and-mouth disease virus in the African buffalo in southern AfricaCorrespondence to: Christopher KasangaEmail: christopher.kasanga@sacids.org Postal address: PO Box 3019, Chuo Kikuu, Morogoro, TanzaniaHow to cite this article: Kasanga, C.J., Dwarka, R., Thobokwe, G., Wadsworth, J., Knowles, N.J., Mulumba, M. et al., 2014, ‘Molecular biological characteristics of foot-and-mouth disease virus in the African buffalo in southern Africa’, Onderstepoort Journal of Veterinary Research 81(2), Art. #728, 1 page. http://dx.doi.org/10.4102/ojvr.v81i2.728  Note: Proceedings of the 2nd One Health Conference in Africa. Jointly organised by the Southern African Centre for Infectious Disease Surveillance and the Tanzania National Institute for Medical Research, held at the Snow Crest Hotel in Arusha, Tanzania from 16th to 19th April 2013: http://www.sacids.org/kms/frontend/index.php?m=119.Copyright: © 2014. The Authors. Licensee: AOSIS OpenJournals. This work is licensed under the Creative Commons Attribution License.

Molecular biological characteristics of foot-and-mouth disease virus in the African buffalo in southern Africa

Molecular biological characteristics of foot-and-mouth disease virus in the African buffalo in southern Africa

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