Histological evaluation on Brazilian green propolis effect in tissue repair of Wistar rats cutaneous wounds

This study aimed to evaluate the action of Brazilian green propolis (5%) topic use on cutaneous wound healing in rats, by inflammatory cell counting. Ten Wistar rats were separated in groups 24 h and 72 h, were trichotomized under anesthesia on cervical-dorsal region, and 2 wounds were provoked with a biopsy punch (5 mm diameter). Left wound underwent Brazilian green propolis topic treatment, and right wounds received nothing. Tissue samples were processed for light microscopy with hematoxilineosin., and leucocytes, macrophages and fibroblasts were counted using a histometric reticule in ocular lens. In both groups, within 24 and 72 h evolution, treated wounds demonstrated significant bigger means for leucocytes, macrophages and fibroblasts. Brazilian green propolis resulted in inflammatory cell quantity increase, suggesting its action on tissue repair process.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Evaluation of the contraction of cutaneous wounds in wistar rats treated with Brazilian green propolis gel

In this work the effects of topic use of Brazilian green propolis gel in the contraction of wounds performed in rat s skin was evaluated. In ten female Wistar rats were done four wounds in dorsal region. Two wounds in each rat were treated daily with Brazilian green propolis gel during 10 days. The wounds were photographed daily, and the images were analyzed with ImageJ software, in order to measure wound areas and evaluate wound contraction. In the graphic analysis, treated group and control group had similar behavior, and both evolved for complete closure in 10th experimentation day. The wound clots fell down before in treated wounds. Green propolis in the gel formulation at 5% in topical use had not effect in the process of wound contraction. The macroscopic visualization of the contraction of wounds, by itself, does not seem to be a good indicator of the process of tissue repair.Colegio de Farmacéuticos de la Provincia de Buenos Aire