Ischemia-Reperfusion Injury of Adipofascial Tissue: An Experimental Study Evaluating Early Histologic and Biochemical Alterations in Rats

Fat necrosis remains a serious complication in reconstructive flaps. In clinical setting, it is well known that fat tissue is more susceptible to ischemic events. We aimed to evaluate early histological and biochemical changes of adipofascial tissue in an experimantal model. An epigastric flap model in rats was used to evaluate the effect of ischemia-reperfusion (I-R) injury on adipofascial tissue. Two groups of animals (one with ischemia alone and other ischemia-reperfusion group) were used to evaluate the degree of histological edema, congestion and extravascular bleeding, and early biochemical alterations within the adipofascial flaps. The biochemical parameters included glutathione (GSH) and malondialdehyde (MDA). In each group, contralateral groin subcutaneous adipose tissue served as control. These evaluations were compared to normal unmanipulated, contralateral abdominal subcutaneous adipose tissue. The ischemia-reperfused flap group showed histologically significantly much edema congestion and bleeding than the control groups (P < .0001). The control group showed less edema in fat tissue than the ischemia-alone group (P < .05). All of the flaps in the ischemia-only group showed significantly less bleeding and edema than I-R group (P < .001). The ratio of MDA/GSH was 33 in control, 37 in ischemia alone, and 82 in ischemia-reperfusion groups, respectively. This study confirms that significant histologic and biochemical alteration occurs after ischemia and ischemia-reperfusion events in adipose tissue. Marked drop in adipose tissue antioxidant levels after I-R suggested that preemptive measures to this decrease should be undertaken in clinical settings

Ischemic preconditioning reduces the severity of ischemia-reperfusion injury of peripheral nerve in rats

<p>Abstract</p> <p>Background and aim</p> <p>Allow for protection of briefly ischemic tissues against the harmful effects of subsequent prolonged ischemia is a phenomennon called as Ischemic Preconditioning (IP). IP has not been studied in ischemia-reperfusion (I/R) model of peripheral nerve before. We aimed to study the effects of acute IP on I/R injury of peripheral nerve in rats.</p> <p>Method</p> <p>70 adult male rats were randomly divided into 5 groups in part 1 experimentation and 3 groups in part 2 experimentation. A rat model of severe nerve ischemia which was produced by tying iliac arteries and all idenfiable anastomotic vessels with a silk suture (6-0) was used to study the effects of I/R and IP on nerve biochemistry. The suture technique used was a slip-knot technique for rapid release at time of reperfusion in the study. Cytoplasmic vacuolar degeneration was also histopathologically evaluated by light microscopic examination in sciatic nerves of rats at 7th day in part 2 study.</p> <p>Results</p> <p>3 hours of Reperfusion resulted in an increase in nerve malondialdehyde levels when compared with ischemia and non-ischemia groups (p < 0.001 and p < 0.0001 respectively). IP had significantly lower nerve MDA levels than 3 h reperfusion group (p < 0.001). The differences between ischemic, IP and non-ischemic control groups were not significant (p > 0.05). There was also a significant decrease in vacoular degeneration of sciatic nerves in IP group than I/R group (p < 0.05).</p> <p>Conclusion</p> <p>IP reduces the severity of I/R injury in peripheral nerve as shown by reduced tissue MDA levels at 3 th hour of reperfusion and axonal vacoulization at 7 th postischemic day.</p

Effects of different types and levels of zinc sulphate applications in vineyards (Vitis vinifera L.) in a semi-arid environment

WOS: 000242775700078The present study was carried out to determine the effect of soil and foliar applications of different rates of zinc in the vicinity of Alasehir, one of the most important viticultural centres in the Western Aegean Region where the round seedless grape cultivar (Vitis vinifera L.) is widely grown, on productivity and some chemical quality characteristics as well as leaf primary and secondary elements. Zinc was applied to soil and foliage as ZnSO4 center dot 7H(2)O in the experiment with four replications. One soil application (0-15-30-45 g ZnSO4 center dot 7H(2)O/vine) in addition to foliar application replicated three times (0.0-0.025-0.050-0.10% Zn). Both soil and foliar zinc sulphate (ZnSO4 center dot 7H(2)O) applications increased the amount of fresh grapes per vine. The highest yield was observed at 30 g ZnSO4 center dot 7H(2)O level per vine in soil application and at the rate of 0.05% Zn in foliar application. Zinc sulphate produced a significant positive effect on the contents of primary and secondary elements (N, P, K, Ca, Mg, Fe, Zn, Mn, Cu) of the leaf (lamina and petiole) with the exception of the leaf petiole manganese content in the soil application and the leaf petiole copper content in the foliar application. Soil and foliar applications of zinc sulphate negatively affected the firmness of the berry flesh. The only fruit quality characteristic positively affected by soil and foliar applications of zinc sulphate was the amount of total soluble solids. Despite the fact that the soil application of zinc sulphate did not have any significant effect on some sugar fractions, the foliar application increased only the fructose and a-glucose significantly. In conclusion, it was established that foliar application of zinc sulphate was slightly more effective on yield as well as some quality characteristics as compared to soil application. Therefore, it was concluded that foliar application could be preferred as it is more economical and easier to apply

QUALITY PARAMETERS OF VINEYARD IRRIGATION WATER IN A SEMI-ARID REGION: THE PLAIN OF ALASEHIR, TURKEY

WOS: 000349805300017Production of seedless raisins is extremely important in Aegean Region in Turkey. The Plain of Alasehir in province of Manisa in Aegean Region is extremely important for seedless raisin production since 25% of the seedless raisin has been grown in this area. The irrigation water samples were collected from 13 different water distribution locations in the Plain of Alasehir. Results showed that pH and EC (electrical conductivity) values were in a reasonable range except EC levels from two locations were slightly high. The vineyard irrigation water in the region was classified as type of C3S1. It was suggested that, the salt content might be monitored continuously since salinity might increase in the soil through the end of the irrigation season. The most common cations were Ca++ and Mg++, and anion was HCO3-. Trace elements and heavy metals were under the risk limits except Mn was high in three locations. Boron was high in nine sampling locations. Overall, irrigation waters in the Plain of Alasehir were suitable for vineyard irrigation as long as the contents of boron and salinity were continuously monitored

A novel therapeutics agent: antioxidant effects of hydroxylfasudil on rat kidney and liver tissues in a protamine sulphate-induced cystitis rat model; preliminary results

Halici, Mesut B./0000-0002-7473-2955; Mercantepe, Tolga/0000-0002-8506-1755; Erol, Huseyin Serkan/0000-0002-9121-536XWOS: 000459181400002PubMed: 29523028Cystitis is defined as an inflammation of the bladder caused by a bacterial infection, and it can be dangerous and painful when it spreads through the internal organs. in this study, antioxidant effects of hydroxylfasudil (HF) at the enzymatic and molecular level on kidney and liver tissues in cystitis rat model, which is caused by inflammation of the rat bladder with a protamine sulphate (PS), was examined. Quantitative changes of reduced glutathione (GSH) and lipid peroxidation (LPO) levels, which are a marker for oxidative stress, were determined in rat kidney and liver tissues for each groups. and then molecular and biochemical impact of HF treatment on antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT) in cystitis model were studied. the results suggest that HF could be beneficial to the renal and hepatic antioxidant system. Thus, HF might be used as a novel therapeutics agent to eliminate interstitial cystitis.Erzincan University Scientific Research and Project UnitsErzincan Binali Yildirim University [11.02.09]This project has been supported by Erzincan University Scientific Research and Project Units, project number "11.02.09'