22 research outputs found
Isolation of non-tuberculous mycobacteria from pastoral ecosystems of Uganda: Public Health significance
<p>Abstract</p> <p>Background</p> <p>The importance of non-tuberculous mycobacteria (NTM) infections in humans and animals in sub-Saharan Africa at the human-environment-livestock-wildlife interface has recently received increased attention. NTM are environmental opportunistic pathogens of humans and animals. Recent studies in pastoral ecosystems of Uganda detected NTM in humans with cervical lymphadenitis and cattle with lesions compatible with bovine tuberculosis. However, little is known about the source of these mycobacteria in Uganda. The aim of this study was to isolate and identify NTM in the environment of pastoral communities in Uganda, as well as assess the potential risk factors and the public health significance of NTM in these ecosystems.</p> <p>Method</p> <p>A total of 310 samples (soil, water and faecal from cattle and pigs) were examined for mycobacteria. Isolates were identified by the INNO-Lipa test and by 16S rDNA sequencing. Additionally, a questionnaire survey involving 231 pastoralists was conducted during sample collection. Data were analysed using descriptive statistics followed by a multivariable logistic regression analysis.</p> <p>Results</p> <p>Forty-eight isolates of NTM were detected; 25.3% of soil samples, 11.8% of water and 9.1% from animal faecal samples contained mycobacteria. Soils around water sources were the most contaminated with NTM (29.8%). Of these samples, <it>M. fortuitum-peregrinum </it>complex, <it>M. avium </it>complex, <it>M. gordonae</it>, and <it>M. nonchromogenicum </it>were the most frequently detected mycobacteria. Drinking untreated compared to treated water (OR = 33), use of valley dam versus stream water for drinking and other domestic use (OR = 20), sharing of water sources with wild primates compared to antelopes (OR = 4.6), sharing of water sources with domestic animals (OR = 5.3), and close contact with cattle or other domestic animals (OR = 13.8) were the most plausible risk factors for humans to come in contact with NTM in the environment.</p> <p>Conclusions</p> <p>The study detected a wide range of potentially pathogenic NTM from the environment around the pastoral communities in Uganda. Drinking untreated water and living in close contact with cattle or other domestic animals may be risk factors associated with the possibility of humans and animals acquiring NTM infections from these ecosystems.</p
The History, Relevance, and Applications of the Periodic System in Geochemistry
Geochemistry is a discipline in the earth sciences concerned with understanding the chemistry of the Earth and what that chemistry tells us about the processes that control the formation and evolution of Earth materials and the planet itself. The periodic table and the periodic system, as developed by Mendeleev and others in the nineteenth century, are as important in geochemistry as in other areas of chemistry. In fact, systemisation of the myriad of observations that geochemists make is perhaps even more important in this branch of chemistry, given the huge variability in the nature of Earth materials – from the Fe-rich core, through the silicate-dominated mantle and crust, to the volatile-rich ocean and atmosphere. This systemisation started in the eighteenth century, when geochemistry did not yet exist as a separate pursuit in itself. Mineralogy, one of the disciplines that eventually became geochemistry, was central to the discovery of the elements, and nineteenth-century mineralogists played a key role in this endeavour. Early “geochemists” continued this systemisation effort into the twentieth century, particularly highlighted in the career of V.M. Goldschmidt. The focus of the modern discipline of geochemistry has moved well beyond classification, in order to invert the information held in the properties of elements across the periodic table and their distribution across Earth and planetary materials, to learn about the physicochemical processes that shaped the Earth and other planets, on all scales. We illustrate this approach with key examples, those rooted in the patterns inherent in the periodic law as well as those that exploit concepts that only became familiar after Mendeleev, such as stable and radiogenic isotopes
Thermodynamic functions of conformational changes I. A comparative first principles study of 1,2-disubstituted ethanes
Gastric fluid volume and pH after nizatidine in adults undergoing elective surgery: influence of timing and dose
The Dialogue Approach to Miranda: Minimizing Disputed Miranda Waivers in the Interrogation Room
The necromass of the Posidonia oceanica seagrass meadow: fate, role, ecosystem services and vulnerability
Earthquake safety in India: achievements, challenges and opportunities
The Indian subcontinent has suffered some of the greatest earthquakes in the world. The earthquakes of the late nineteenth and early twentieth centuries triggered a number of early advances in science and engineering related to earthquakes that are discussed here. These include the development of early codes and earthquake-resistant housing after the 1935 Quetta earthquake in Baluchistan, and strengthening techniques implemented after the 1941 Andaman Islands earthquake, discovered by the author in remote islands of India. Activities in the late 1950s to institutionalize earthquake engineering in the country are also discussed. Despite these early developments towards seismic safety, moderate earthquakes in India continue to cause thousands of deaths, indicating the poor seismic resilience of the built environment. The Bhuj earthquake of 2001 highlighted a striking disregard for structural design principles and quality of construction. This earthquake was the first instance of an earthquake causing collapses of modern multi-storey buildings in India, and it triggered unprecedented awareness amongst professionals, academics and the general public. The earthquake led to the further development of the National Information Centre of Earthquake Engineering and the establishment of a comprehensive 4-year National Programme on Earthquake Engineering Education that was carried out by the seven Indian Institutes of Technology and the Indian Institute of Science. Earthquake engineering is a highly context-specific discipline and there are many engineering problems where appropriate solutions need to be found locally. Confined masonry construction is one such building typology that the author has been championing for the subcontinent. Development of the student hostels and staff and faculty housing on the new 400-acre campus of the Indian Institute of Technology Gandhinagar has provided an opportunity to adopt this construction typology on a large scale, and is addressed in the monograph. The vulnerability of the building stock in India is also evident from the occasional news reports of collapses of buildings under construction or during rains (without any earthquake shaking). Given India’s aspirations to be counted as one of the world’s prosperous countries, there is a great urgency to address the safety of our built environment. There is a need: to create a more professional environment for safe construction, including a system for code enforcement and building inspection; for competence-based licensing of civil and structural engineers; for training and education of all stakeholders in the construction chain; to build a research and development culture for seismic safety; to encourage champions of seismic safety; to effectively use windows of opportunity provided by damaging earthquakes; to focus on new construction as opposed to retrofitting existing buildings; and to frame the problem in the broader context of overall building safety rather than the specific context of earthquakes. Sustained long-term efforts are required to address this multi-faceted complex problem of great importance to the future development of India. While the context of this paper is India, many of the observations may be valid and useful for other earthquake-prone countriesby Sudhir K. Jai
Cellular Origin, Life in Extreme Habitats and Astrobiology
The problem of the origin of metazoa is becoming more urgent in the context of astrobiology. By now it is clear that clues to the understanding of this crucial transition in the evolution of life can arise in a fourth pathway besides the three possibilities in the quest for simplicity outlined by Bonner in his classical book. In other words, solar system exploration seems to be one way in the long-term to elucidate the simplicity of evolutionary development. We place these ideas in the context of different inheritance systems, namely the genotypic and phenotypic replicators with limited or unlimited heredity, and ask which of these can support multicellular development, and to which degree of complexity. However, the quest for evidence on the evolution of biotas from planets around other stars does not seem to be feasible with present technology with direct visualization of living organisms on exoplanets. But this may be attempted on the Galilean moons of Jupiter where there is a possibility of detecting reliable biomarkers in the next decade with the Europa Jupiter System Mission, in view of recent progress by landing micropenetrators on planetary, or satellite surfaces. Mars is a second possibility in the inner Solar System, in spite of the multiple difficulties faced by the fleet of past, present and future missions. We discuss a series of preliminary ideas for elucidating the origin of metazoan analogues with available instrumentation in potential payloads of feasible space missions to the Galilean moons