Simple model for the phase coexistence and electrical conductivity of alkali fluids

We report the first theoretical model for the alkali fluids which yields a liquid-vapor phase coexistence with the experimentally observed features and electrical conductivity estimates which are also in accord with observations. We have carried out a Monte Carlo simulation for a lattice gas model which allows an integrated study of the structural, thermodynamic, and electronic properties of metal-atom fluids. Although such a technique is applicable to both metallic and nonmetallic fluids, non-additive interactions due to valence electron delocalization are a crucial feature of the present model.Comment: RevTex, 11 pages, 2 ps figure files appended, submitted to PR

Study of theoretical models for the liquid-vapor and metal-nonmetal transitions of alkali fluids

Theoretical models for the liquid-vapor and metal-nonmetal transitions of alkali fluids are investigated. Mean-field models are considered first but shown to be inadequate. An alternate approach is then studied in which each statistical configuration of the material is treated as inhomogeneous, with the energy of each ion being determined by its local environment. Nonadditive interactions, due to valence electron delocalization, are a crucial feature of the model. This alternate approach is implemented within a lattice-gas approximation which takes into account the observed mode of expansion in the materials of interest and which is able to treat the equilibrium density fluctuations. We have carried out grand canonical Monte Carlo simulations, for this model, which allow a unified, self-consistent, study of the structural, thermodynamic, and electronic properties of alkali fluids. Applications to Cs, Rb, K, and Na yield results in good agreement with observations.Comment: 13 pages, REVTEX, 10 ps figures available by e-mail

RNA-binding protein Elavl1/HuR is required for maintenance of cranial neural crest specification

Neural crest development is transcriptionally controlled via sequential activation of gene regulatory networks (GRNs). Recent evidence increasingly implicates a role for post-transcriptional regulation in modulating the output of these regulatory circuits. Using RNA-sequencing data from avian embryos to identify potential post-transcriptional regulators, we observed enrichment during early neural crest development of Elavl1, which encodes for the RNA-binding protein HuR. Immunohistochemical analyses revealed expression of HuR following establishment of the neural plate border. Perturbation of HuR resulted in premature neural crest delamination from the neural tube as well as significant reduction in transcripts associated with the neural crest specification GRN (Axud1 and FoxD3), phenotypes also observed with downregulation of the canonical Wnt inhibitor Draxin. RNA pulldown further shows that Draxin is a specific target of HuR. Importantly, overexpression of exogenous Draxin was able to rescue the cranial neural crest specification defects observed with HuR knockdown. Thus, HuR plays a critical a role in the maintenance of cranial neural crest specification, at least partially via Draxin mRNA stabilization. Together, these data highlight an important intersection of post-transcriptional regulation with modulation of the neural crest specification GRN

RNA-binding protein Elavl1/HuR is required for maintenance of cranial neural crest specification

Neural crest development is transcriptionally controlled via sequential activation of gene regulatory networks (GRNs). Recent evidence increasingly implicates a role for post-transcriptional regulation in modulating the output of these regulatory circuits. Using RNA-sequencing data from avian embryos to identify potential post-transcriptional regulators, we observed enrichment during early neural crest development of Elavl1, which encodes for the RNA-binding protein HuR. Immunohistochemical analyses revealed expression of HuR following establishment of the neural plate border. Perturbation of HuR resulted in premature neural crest delamination from the neural tube as well as significant reduction in transcripts associated with the neural crest specification GRN (Axud1 and FoxD3), phenotypes also observed with downregulation of the canonical Wnt inhibitor Draxin. RNA pulldown further shows that Draxin is a specific target of HuR. Importantly, overexpression of exogenous Draxin was able to rescue the cranial neural crest specification defects observed with HuR knockdown. Thus, HuR plays a critical a role in the maintenance of cranial neural crest specification, at least partially via Draxin mRNA stabilization. Together, these data highlight an important intersection of post-transcriptional regulation with modulation of the neural crest specification GRN

The ultracool dwarf DENIS-P J104814.7-395606. Chromospheres and coronae at the low-mass end of the main-sequence

We have obtained an XMM-Newton observation and a broad-band spectrum from the ultraviolet to the near infrared with X-Shooter for one of the nearest M9 dwarfs, DENIS-P J1048-3956 (4pc). We integrate these data by a compilation of activity parameters for ultracool dwarfs from the literature with the aim to advance our understanding of these objects by comparing them to early-M type dwarf stars and the Sun. Our deep XMM-Newton observation has led to the first X-ray detection of DENIS-P J1048-3956 (log Lx = 25.1) as well as the first measurement of its V band brightness (V = 17.35mag). Flux-flux relations between X-ray and chromospheric activity indicators are here for the first time extended into the regime of the ultracool dwarfs. The approximate agreement of DENIS-P J1048-3956 and other ultracool dwarfs with flux-flux relations for early-M dwarfs suggests that the same heating mechanisms work in the atmospheres of ultracool dwarfs, albeit weaker as judged from their lower fluxes. The observed Balmer decrements of DENIS-P J1048-3956 are compatible with optically thick plasma in LTE at low, nearly photospheric temperature or optically thin LTE plasma at 20000K. Describing the decrements with CaseB recombination requires different emitting regions for Halpha and the higher Balmer lines. The high observed Halpha/Hbeta flux ratio is also poorly fitted by the optically thin models. We derive a similarly high value for the Halpha/Hbeta ratio of vB10 and LHS2065 and conclude that this may be a characteristic of ultracool dwarfs. We add DENIS-P J1048-3956 to the list of ultracool dwarfs detected in both the radio and the X-ray band. The Benz-Guedel relation between radio and X-ray luminosity of late-type stars is well-known to be violated by ultracool dwarfs. We speculate on the presence of two types of ultracool dwarfs with distinct radio and X-ray behavior.Comment: accepted for publication in Astronomy & Astrophysic

Mapping deuterated methanol toward L1544: I. Deuterium fraction and comparison with modeling

The study of deuteration in pre-stellar cores is important to understand the physical and chemical initial conditions in the process of star formation. In particular, observations toward pre-stellar cores of methanol and deuterated methanol, solely formed on the surface of dust grains, may provide useful insights on surface processes at low temperatures. Here we analyze maps of CO, methanol, formaldehyde and their deuterated isotopologues toward a well-known pre-stellar core. This study allows us to test current gas-dust chemical models. Single-dish observations of CH$_3$OH, CH$_2$DOH, H$_2$CO, H_2\,^{13}CO, HDCO, D$_2$CO and C$^{17}$O toward the prototypical pre-stellar core L1544 were performed at the IRAM 30 m telescope. We analyze their column densities, distributions, and compare these observations with gas-grain chemical models. The maximum deuterium fraction derived for methanol is [CH$_2$DOH]/[CH$_3$OH] $\sim$ 0.08$\pm$0.02, while the measured deuterium fractions of formaldehyde at the dust peak are [HDCO]/[H$_2$CO] $\sim$ 0.03$\pm$0.02, [D$_2$CO]/[H$_2$CO] $\sim$ 0.04$\pm$0.03 and [D$_2$CO]/[HDCO] $\sim$ 1.2$\pm$0.3. Observations differ significantly from the predictions of models, finding discrepancies between a factor of 10 and a factor of 100 in most cases. It is clear though that to efficiently produce methanol on the surface of dust grains, quantum tunneling diffusion of H atoms must be switched on. It also appears that the currently adopted reactive desorption efficiency of methanol is overestimated and/or that abstraction reactions play an important role. More laboratory work is needed to shed light on the chemistry of methanol, an important precursor of complex organic molecules in space.Comment: Accepted for publication in A&

Type I cAMP-Dependent Protein Kinase Delays Apoptosis in Human Neutrophils at a Site Upstream of Caspase-3

Current data suggest that apoptosis controls neutrophil numbers in tissues. We analyzed roles for and the sites of action for the cAMP-dependent protein kinases (cAPKs) in apoptosis induced in human neutrophils by in vitro storage, cycloheximide (CHX) exposure, and anti-Fas exposure. Treatment with 8-chlorophenylthio-cAMP (8-CPT-cAMP) prolonged the time required for 50% of the cells to exhibit apoptotic morphology (t 50) from 16.3 to 41.8 h (in vitro culture), from 2.4 to 7.8 h (CHX), and from 4.8 to 6.5 h (anti-Fas). CHX ± 8-CPT-cAMP did not significantly alter resting intracellular calcium levels and H-89, a selective inhibitor of cAPK, had no effect on apoptosis in the absence of the analogue. In contrast, site-selective cAMP analogues that specifically activated the type I cAPK, but not type II cAPK, synergistically attenuated apoptosis. Exposure to 8-CPT-cAMP delayed, in parallel, the activity of caspase-3 (CPP-32β), whereas mitogen-activated protein kinase kinase (MAPKK) inhibitor, PD98059, had no effect on CHX-induced apoptosis ± 8-CPT-cAMP. Together these results indicate that type I cAPK activation is necessary and sufficient to mediate cAMP-induced delay in human neutrophil apoptosis induced by several mechanisms and suggest that one of the major sites of cAPK action is upstream of caspase-3 (CPP-32β) activation

Microscopic Surface Structure of Liquid Alkali Metals

We report an x-ray scattering study of the microscopic structure of the surface of a liquid alkali metal. The bulk liquid structure factor of the eutectic K67Na33 alloy is characteristic of an ideal mixture, and so shares the properties of an elemental liquid alkali metal. Analysis of off-specular diffuse scattering and specular x-ray reflectivity shows that the surface roughness of the K-Na alloy follows simple capillary wave behavior with a surface structure factor indicative of surface induced layering. Comparison of thelow-angle tail of the K67Na33 surface structure factor with the one measured for liquid Ga and In previously suggests that layering is less pronounced in alkali metals. Controlled exposure of the liquid to H2 and O2 gas does not affect the surface structure, indicating that oxide and hydride are not stable at the liquid surface under these experimental conditions.Comment: 12 pages, 3 figures, published in Phys. Rev.

Power Quality Aspects in a Wind Power Plant: Preprint

Although many operational aspects affect wind power plant operation, this paper focuses on power quality. Because a wind power plant is connected to the grid, it is very important to understand the sources of disturbances that affect the power quality

An Arbitrary Curvilinear Coordinate Method for Particle-In-Cell Modeling

A new approach to the kinetic simulation of plasmas in complex geometries, based on the Particle-in- Cell (PIC) simulation method, is explored. In the two dimensional (2d) electrostatic version of our method, called the Arbitrary Curvilinear Coordinate PIC (ACC-PIC) method, all essential PIC operations are carried out in 2d on a uniform grid on the unit square logical domain, and mapped to a nonuniform boundary-fitted grid on the physical domain. As the resulting logical grid equations of motion are not separable, we have developed an extension of the semi-implicit Modified Leapfrog (ML) integration technique to preserve the symplectic nature of the logical grid particle mover. A generalized, curvilinear coordinate formulation of Poisson's equations to solve for the electrostatic fields on the uniform logical grid is also developed. By our formulation, we compute the plasma charge density on the logical grid based on the particles' positions on the logical domain. That is, the plasma particles are weighted to the uniform logical grid and the self-consistent mean electrostatic fields obtained from the solution of the logical grid Poisson equation are interpolated to the particle positions on the logical grid. This process eliminates the complexity associated with the weighting and interpolation processes on the nonuniform physical grid and allows us to run the PIC method on arbitrary boundary-fitted meshes.Comment: Submitted to Computational Science & Discovery December 201