9 research outputs found
Additional file 1: Figure S1. of Mitosis in circulating tumor cells stratifies highly aggressive breast carcinomas
Common recognizable Cytologies of CTCs in Mitosis isolated from breast cancer patients with all the “standard” CTC stains from Fig. 1. Figure S2. Common recognizable Cytologies of CTCs in Mitosis isolated from breast cancer patients with all the “standard” CTC stains from Fig. 1. Figure S3. Kaplan-Meier estimates of probabilities of Overall Survival of the patient subpopulations based on receptor status from Fig. 2a (n=33). Figure S4. Box plot of total number of CTCs in each patient versus mitotic CTCs for each patient. Figure S5. CTC counts and Mitotic CTC counts for each patient sample in relation to time of filtration after blood draw. Table S1. Patient subpopulations classified by stage, receptor status and treatment. (PDF 748 kb
Her2-FISH analysis of DTCs from breast cancer patient BM.
<p>Top Row: DTC with normal copy number of CEP17 and Her2. Bottom Row: DTC with normal copy number of CEP17 and increased copy number of Her2.</p
Recovery efficiency of breast cancer cells from bone marrow spiking experiments.
<p>Recovery efficiency of breast cancer cells from bone marrow spiking experiments.</p
Expression of DTC associated transcripts in filtered and unfiltered BM specimens.
<p>Expression of 11 genes in normal BM samples spiked with 20 MDA-MB-231 cells per million nucleated BM cells compared to unspiked normal BM (NBM) as shown in x-axis. RNA expression was measured in either filtered (F) or unfiltered (UF) BM samples. Gene expression was determined by qRT-PCR on a Fluidigm platform. The actual fold values over normal BM are depicted.</p
Recovery and fold enrichment of indicated number of SKBR3 tumor cells spiked into an initial sample of 14 million BM cells.
<p>Recovery and fold enrichment of indicated number of SKBR3 tumor cells spiked into an initial sample of 14 million BM cells.</p
RNA-In situ hybridization for ERBB2 gene expression.
<p>BM from 2 breast cancer patients after filtration analyzed by RNA-ISH for Her-2 expression. Her2/ERBB2 RNA-ISH positive cells are brown (arrows).</p
Images of the filter-captured breast cancer tissue culture cells.
<p>Fig 1A. (A) Normal bone marrow control cells stained on the microfilter (B) Normal bone marrow cells spiked with SKBR3 breast cancer cells stained on the microfilter. Arrows indicate SKBR3 cells. Fig 1B. Antibody staining of SKBR3 breast cancer cells after filtration. Nuclei are shown as blue in the merged images.</p
Appearance of filter-captured DTCs from BC patient bone marrow by fluorescent antibody staining.
<p>(A, B), Filter-captured DTCs; (C), Hematopoietic precursor cells (HPC). Nuclei are shown as blue in the merged images.</p