原著和名: [記載なし]科名: = unknown採集地:採集日:採集者:整理番号: JH043720国立科学博物館整理番号: TNS-VS-99372

Cha-Mei Tang (3421535)

Daniel Adams (3421532)

Erik Castle (3493622)

Melissa Stanton (3493625)

Peixuan Zhu (3493613)

Platte Amstutz (3493616)

Richard Joseph (3493610)

Shuhong Li (151480)

Thai Ho (3493619)

Additional file 6: Figure S5. A mitotic CTC in a cell cluster detected in frozen RCC Patient 2 sample. The image is cropped to show the mitotic CTC. The CTC is under cell division (red arrow). The mitotic CTC is seen at telophase because the nuclei have been separated into 2 sets of chromosomes (light blue) and share a common cytoplasm (green)

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MOESM6 of Detection of tumor-associated cells in cryopreserved peripheral blood mononuclear cell samples for retrospective analysis

Additional file 4: Figure S3. Effect of storage temperature on peripheral blood mononuclear cell (PBMC) samples. (A) MCF7; (B) 786-O; (C) PBMC. The PBMC cells were prepared from whole blood samples collected in heparin tube from healthy controls by using standard Ficoll protocols. The PBMCs were resuspended in cryofluid to a final concentration of 1 million/mL and then aliquoted to 1 mL per vial in cryotube. Breast cancer cell line, MCF7 and RCC cell line, 786-O were spiked in the PBMC at ratio of 100 tumor cells per million of PBMC, respectively. The spiked PBMC samples (n = 3 for each condition) were stored at −80 °C and liquid nitrogen. After three weeks of storage, the samples were thawed out and processed through standard CellSieve™ microfiltration and antibody staining. No cell damage was observed after storage for three weeks in lower temperature condition in liquid nitrogen. We did not find differences in cell morphology and antibody staining patterns between the cells stored at −80 °C and liquid nitrogen

MOESM4 of Detection of tumor-associated cells in cryopreserved peripheral blood mononuclear cell samples for retrospective analysis

https://figshare.com/articles/MOESM4_of_Detection_of_tumor-associated_cells_in_cryopreserved_peripheral_blood_mononuclear_cell_samples_for_retrospective_analysis/4390931

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