Avaliação do efeito da radiação ultravioleta “A” sobre porcentagens de fagocitose em hemócitos e concentração da glicose na hemolinfa do camarão-rosa Farfantepenaeus paulensis

The crustacean are naturally exposed to ultraviolet radiation, it can produce biochemical and in immune function changes and predispose to diseases manifestation. Objective: To quantify the haemolymph glucose concentration and evaluate the phagocitary index of haemocytes in shrimp Farfantepenaeus paulensis exposed to UVA irradiation. Methodology: 21 shrimps were divided in one control group and 3 treatment groups exposed to UVA radiation for 1, 5 and 10 days. Phagocytosis was carried out in slide glass, the haemocytes capacity to engulf heat-killed yeast (Saccharomyces cerevisae) were analyzed. Glucose was determined by enzymatic method (oxidase glucose). The results were expressed by mean ± standard deviations and analyzed by ANOVA follow Tuckey test. Results: Phagocytosis (%) and the glucose concentration (mmol/l) of the animals exposed for 1, 5 and 10 days and control group were respectively, 71.70±2.03 and 0.36±0.19; 70.0±4.73 and 0.88±0.47; 63.80±3.67 and 0.18±0.03 and 85.65±2.81 and 2.41±0.74. Control group showed higher phagocytosis index (P>0.05) and the exposition during 10 days impaired the index (P>0.05). It was observed lower concentration of glucose in groups exposed than to control group (P>0.05). Conclusion: The results reached with the hypothesis of immunosuppression by the exposition to UVA.Os crustáceos estão naturalmente expostos à radiação ultravioleta, podendo esta produzir mudanças bioquímicas e na função imune e predispor ao aparecimento de doenças. Objetivo: Quantificar a concentração de glicose na hemolinfa e avaliar o índice fagocitário em hemócitos de camarões Farfantepenaeus paulensis expostos à radiação UVA. Metodologia: 21 camarões foram divididos em três grupos submetidos à radiação UVA (tratamentos de 1, 5 e 10 dias) e grupo controle. A fagocitose foi efetuada em lâminas, nas quais os hemócitos foram avaliados pela sua capacidade de ingerir leveduras (Sacharomyces cerevisae). A glicose foi determinada por método enzimático (glicose oxidase). Os resultados foram expressos como média ± desvio-padrão e efetuada análise de variância e teste de Tuckey “a posteriori”. Resultados: A fagocitose (%) e a concentração de glicose (mmol/l) dos animais expostos por 1, 5 e 10 dias e do grupo controle foram respectivamente, 71,70±2,03 e 0,36±0,19; 70,0±4,73 e 0,88±0,47; 63,80±3,67 e 0,18±0,03 e 85,65±2,81 e 2,41±0,74. O grupo controle apresentou maior índice fagocitário (P>0,05) e os animais expostos durante 10 dias mostraram o índice inferior (P>0,05). Observou-se menor concentração de glicose em todos os grupos expostos em relação ao controle (P>0,05). Conclusão: Os resultados corroboram a hipótese de imunodepressão pela exposição à UVA

The use of microorganisms as food source for Penaeus paulensis larvae

Submitted by Thainã Moraes ([email protected]) on 2013-04-13T00:22:32Z No. of bitstreams: 1 THE USE OF MICROORGANISMS AS FOOD SOURCE FOR PENAEUS PAULENSIS LARVAE.pdf: 200397 bytes, checksum: 43e47c4868be379a5fe65f0325088b70 (MD5)Approved for entry into archive by Michele Fernanda([email protected]) on 2013-08-20T21:15:33Z (GMT) No. of bitstreams: 1 THE USE OF MICROORGANISMS AS FOOD SOURCE FOR PENAEUS PAULENSIS LARVAE.pdf: 200397 bytes, checksum: 43e47c4868be379a5fe65f0325088b70 (MD5)Made available in DSpace on 2013-08-20T21:15:33Z (GMT). No. of bitstreams: 1 THE USE OF MICROORGANISMS AS FOOD SOURCE FOR PENAEUS PAULENSIS LARVAE.pdf: 200397 bytes, checksum: 43e47c4868be379a5fe65f0325088b70 (MD5) Previous issue date: 1999Three experiments were conducted to test the usefulness of microorganisms as food source for the Penaeus paulensis larvae. Larvae fed only bacteria survived longer _3 days. than those cultured in filtered _-1.0 mm. seawater. However, they grew better when fed flagellates and ciliates, reaching the protozoa II stage after 8 days. Gut content analysis showed that the ciliate Cristigera minuta was heavily grazed by the larvae. The addition of microalgae and Artemia sp. nauplii besides microorganisms did not result in increased survival. However, all larvae that received supplementary food in the form of flagellates and ciliates showed larger cephalotorax length than those in the control treatments. q1999 Elsevier Science B.V. All rights reserved

The use of microorganisms as food source for Penaeus paulensis larvae

Three experiments were conducted to test the usefulness of microorganisms as food source for the Penaeus paulensis larvae. Larvae fed only bacteria survived longer _3 days. than those cultured in filtered _-1.0 mm. seawater. However, they grew better when fed flagellates and ciliates, reaching the protozoa II stage after 8 days. Gut content analysis showed that the ciliate Cristigera minuta was heavily grazed by the larvae. The addition of microalgae and Artemia sp. nauplii besides microorganisms did not result in increased survival. However, all larvae that received supplementary food in the form of flagellates and ciliates showed larger cephalotorax length than those in the control treatments. q1999 Elsevier Science B.V. All rights reserved

Nursery of the pink shrimp Farfantepenaeus paulensis in cages with artificial substrates: Biofilm composition and shrimp performance

The use of artificial substrate (polyethylene screens) was investigated during the nursery rearing of the pink shrimp Farfantepenaeus paulensis cultured in fixed cages installed in a shallow estuarine inlet of the Patos Lagoon, southern Brazil. High water renewal rates during the experimental period kept water quality in the suitable range for penaeid shrimp culture. Although shrimp were fed a high quality artificial diet (40% protein) throughout the experiment, they were observed to occupy the substrates and actively graze on the biofilm. The bulk of the biofilm was composed of diatoms, filamentous cyanobacteria, protozoa and nematodes. Biofilm chlorophyll a concentration was significantly affected by shrimp grazing pressure and the composition of the diatom community suffered significant changes after shrimp introduction to the cages. At the end of the trial shrimp reared in the presence of artificial substrates achieved significant higher survival, growth and total biomass (pb0.05). Biofilm was likely an additional source of food for the reared animals

Effect of biofloc technology (BFT) on the early postlarval stage of pink shrimp Farfantepenaeus paulensis: growth performance, floc composition and salinity stress tolerance

Biofloc rearing media provides a potential food source for shrimp reared in limited or zero water exchange systems. This culture system is environmentally friendly as it is based on limited water use and minimal effluent is released into the surrounding environment. In this study, we evaluated the survival, growth performance and salinity stress tolerance of pink shrimp Farfantepenaeus paulensis postlarvae reared from PL10 to PL25 in a biofloc technology limited water exchange system. PL (mean ± SD weight and length of 14 ± 10 mg and 8.10 ± 0.7 mm, respectively) were reared in nine 40-L plastic tanks with a stocking density of 10PL/L. Three culture treatments were applied (1) culture in the presence of bioflocs and commercial feed supply (FLOC ? CF); (2) culture in the presence of biofloc without feed supply (FLOC) and (3) culture in clear water with feed supply (control). Final biomass and survival were significantly higher in FLOC ? CF treatment than the control (P \ 0.05), but did not differ from FLOC. PL reared in the FLOC ? CF treatment achieved a significantly higher final weight, weight gain and length in comparison with the other two treatments (P \ 0.05). No significant difference(P [ 0.05) between treatments was found for salinity tolerance over 24 and 48 h durations. The proximate analysis of floc shown high levels of crude protein (30.4%), but low levels of crude lipids (0.5%). The continuous availability of bioflocs had a significant effect on growth and survival of F. paulensis postlarvae cultured in BFT nursery systems

Influence of the biofilm on the growth of the pink shrimp Farfantepenaeus paulensis reared in nursery systems

Submitted by Cristina Damasceno ([email protected]) on 2012-06-18T18:28:08Z No. of bitstreams: 1 Influência do biofilme no crescimento do camarão-rosa Farfantepenaeus paulensis em sistema de berçário.pdf: 292402 bytes, checksum: 3331207f40a8e55809e65fa8209027a8 (MD5)Approved for entry into archive by Sabrina Andrade([email protected]) on 2013-05-31T20:55:29Z (GMT) No. of bitstreams: 1 Influência do biofilme no crescimento do camarão-rosa Farfantepenaeus paulensis em sistema de berçário.pdf: 292402 bytes, checksum: 3331207f40a8e55809e65fa8209027a8 (MD5)Made available in DSpace on 2013-05-31T20:55:29Z (GMT). No. of bitstreams: 1 Influência do biofilme no crescimento do camarão-rosa Farfantepenaeus paulensis em sistema de berçário.pdf: 292402 bytes, checksum: 3331207f40a8e55809e65fa8209027a8 (MD5) Previous issue date: 2003O objetivo deste trabalho foi avaliar a influência do biofilme sobre o crescimento de pós-larvas do camarão-rosa Farfantepenaeus paulensis em sistema de gaiolas-berçários localizadas em uma enseada rasa do estuário da Lagoa dos Patos - RS. Pós-larvas de 25 dias (PL25) com peso médio de 14 mg, foram estocados na densidade de 300 camarões/m2 em gaiolas com 4m2 de fundo, entre 23 de fevereiro e 25 de março de 2001. Três gaiolas formaram o tratamento “com biofilme” – CB e foram colocadas no ambiente dez dias antes do início do experimento. Outras três gaiolas compuseram o tratamento “sem biofilme” – SB onde a panagem foi trocada a cada 10 dias, minimizando a fixação do biofilme. Ao final do experimento constatou-se que não houve diferença significativa (p>0,05) entre as sobrevivências médias dos camarões nos dois tratamentos, entretanto as pós-larvas do tratamento CB tiveram um crescimento (peso úmido) significativamente maior (p<0,05) que aquelas do tratamento SB onde a fixação do biofilme foi limitada.This study evaluated the influence of biofilm on the growth of the pink shrimp Farfantepenaeus paulensis reared in nursery cages in a shallow estuarine inlet of Patos Lagoon, southern Brazil. F. paulensis postlarvae (PL25, mean weight 14 mg) were stocked at a density of 300 shrimps/m2, from February, 23 to March 25; 2001. The experimental design consisted of two treatments: in the first one, three 4m2 cages were installed in the estuarine inlet 10 days before the beginning of the experiment and the biofilm attached to them was not cleaned off throughout the experimental period. In the second treatment, three cages were exchanged every 10 days in order to limit biofilm attachment. At the end of the experiment, there was no significant difference in the survival between treatments, but postlarvae from the treatment with biofilm had a significantly (p<0.05) higher growth than those reared in cages where biofilm attachment was reduced

Influência do biofilme no crescimento do camarão-rosa Farfantepenaeus paulensis em sistema de berçário

O objetivo deste trabalho foi avaliar a influência do biofilme sobre o crescimento de pós-larvas do camarão-rosa Farfantepenaeus paulensis em sistema de gaiolas-berçários localizadas em uma enseada rasa do estuário da Lagoa dos Patos - RS. Pós-larvas de 25 dias (PL25) com peso médio de 14 mg, foram estocados na densidade de 300 camarões/m2 em gaiolas com 4m2 de fundo, entre 23 de fevereiro e 25 de março de 2001. Três gaiolas formaram o tratamento “com biofilme” – CB e foram colocadas no ambiente dez dias antes do início do experimento. Outras três gaiolas compuseram o tratamento “sem biofilme” – SB onde a panagem foi trocada a cada 10 dias, minimizando a fixação do biofilme. Ao final do experimento constatou-se que não houve diferença significativa (p>0,05) entre as sobrevivências médias dos camarões nos dois tratamentos, entretanto as pós-larvas do tratamento CB tiveram um crescimento (peso úmido) significativamente maior (p<0,05) que aquelas do tratamento SB onde a fixação do biofilme foi limitada.This study evaluated the influence of biofilm on the growth of the pink shrimp Farfantepenaeus paulensis reared in nursery cages in a shallow estuarine inlet of Patos Lagoon, southern Brazil. F. paulensis postlarvae (PL25, mean weight 14 mg) were stocked at a density of 300 shrimps/m2, from February, 23 to March 25; 2001. The experimental design consisted of two treatments: in the first one, three 4m2 cages were installed in the estuarine inlet 10 days before the beginning of the experiment and the biofilm attached to them was not cleaned off throughout the experimental period. In the second treatment, three cages were exchanged every 10 days in order to limit biofilm attachment. At the end of the experiment, there was no significant difference in the survival between treatments, but postlarvae from the treatment with biofilm had a significantly (p<0.05) higher growth than those reared in cages where biofilm attachment was reduced

Efeito da densidade de estocagem sobre o biofilme e o desempenho de pós-larvas do camarão-rosa Farfantepenaeus paulensis cultivadas em gaiolas

O objetivo deste estudo foi analisar a influência da densidade de estocagem na sobrevivência e no crescimento de pós-larvas de F. paulensis cultivadas em gaiolas durante a fase de berçário. Além disso, foram identificados e quantificados os principais microrganismos presentes no biofilme formado nos substratos artificiais colocados dentro das gaiolas. Foram usadas gaiolas com 4 m2 de fundo e com substratos artificiais, nas densidades de 100, 200,300, 400 e 500PL25 m-2. O experimento durou 35 dias. Observou-se uma relação negativa entre o aumento da densidade de estocagem de camarões e a sobrevivência e o crescimento destes. Foi observada uma preferência pelo consumo de diatomáceas cêntricas. A viabilidade técnica do cultivo de F. paulensis em gaiolas foi confirmada. O cultivo de F. paulensis em gaiolas na fase de berçário pode ser feito em densidades de até 400PL m-2, sem maiores perdas em sua sobrevivência e seu crescimento.The aim of this study was to analyze the influence of the stocking density in the survival and growth of Farfantepenaeus paulensis postlarvae reared in cages during the nursery phase. Additionally, widentified and quantified the main microorganisms present on the biofilm formed on artificial substrates placed inside the cages. We used 4 m2 bottom surface cages with artificial substrates and shrimp stocking densities of 100, 200, 300, 400 and 500PL25 m-2. The experiment lasted for 35 days. A negative relationship was observed between the increase of shrimp stocking density and their survival/growth rates. A preference for centric diatoms was also observed. The technical feasibility of culturing F. paulensis in cages was confirmed. The rearing of F. paulensis in nursery cages may be conduced in densities at also 400PL m-2, without more loss in survival and growth