How to Write Comedy for Radio

Entertainment and television icon Johnny Carson received his bachelor of arts degree in radio and speech (with a minor in physics) in 1949 from the University of Nebraska. While at Nebraska, he was a member of Phi Gamma Delta fraternity and served as master of ceremonies for the Kosmet Klub, a male dramatic society. Before completing his bachelor of arts degree, Carson did a senior thesis for his broadcasting professor, William Dempsey. His 1949 senior thesis was entitled “How to Write Comedy for Radio,” which he recorded on a reel-to-reel tape. The 45-minute recording was a scholarly examination of the techniques and devices that radio comedy writers used to construct the jokes and gags in comedy radio shows. Using bits from several well-known comedians, such as Jack Benny and Bob Hope, Carson illustrated the various techniques used to write comedy, which he later effectively used in television through his “Tonight Show” monologues. Born in Corning, Iowa, on Oct. 23, 1925, Carson grew up in Norfolk, Neb. He died on Jan. 23, 2005. Carson became host of “The Tonight Show” in October of 1962. He retired in 1992 after hosting more than 4,531 shows. In November 2004, Carson announced a gift of $5.3 million to the University of Nebraska Foundation to support the renovation and expansion of the Temple Building, where he got his start, and to create an endowment to keep performance spaces equipped with the latest advances in lighting and sound technologies. Following Carson’s death in January 2005, the University of Nebraska Foundation received an additional gift of$5 million from the estate of Carson for endowed support of programs in theatre, film and broadcasting. Annual income from the endowment provides support to the Johnny Carson School of Theatre and Film in the Hixson-Lied College of Fine and Performing Arts and the broadcast program in the College of Journalism and Mass Communications. In August 2005, the University of Nebraska renamed the Department of Theatre Arts the Johnny Carson School of Theatre and Film, creating a permanent legacy for Johnny at the University of Nebraska. The complete 45-minute monologue is available as the Related File linked above. It is a downloadable mp3 file of 69 Mbytes

Biologic effects of oil fly ash.

Epidemiologic studies have demonstrated increased human morbidity and mortality with elevations in the concentration of ambient air particulate matter (PM). Fugitive fly ash from the combustion of oil and residual fuel oil significantly contributes to the ambient air particle burden. Residual oil fly ash (ROFA) is remarkable in the capacity to provoke injury in experimental systems. The unique composition of this emission source particle makes it particularly useful as a surrogate for ambient air PM in studies of biologic effects testing the hypothesis that metals mediate the biologic effects of air pollution particles. A majority of the in vitro and animal model investigations support the postulate that transition metals present in ROFA (especially vanadium) participate in Fenton-like chemical reactions to produce reactive oxygen species. This is associated with tyrosine phosphorylation, nuclear factor kappa B and other transcription factor activation, induction of inflammatory mediator expression, and inflammatory lung injury. It is also evident that vanadium accounts for a significant portion of the biologic activity of ROFA. The extrapolation of this body of investigation on ROFA to the field of ambient air PM is difficult, as particles in numerous environments have such small amounts of vanadium

Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy

Freeze-fracture/freeze-etch describes a process whereby specimens, typically biological or nanomaterial in nature, are frozen, fractured, and replicated to generate a carbon/platinum “cast” intended for examination by transmission electron microscopy. Specimens are subjected to ultrarapid freezing rates, often in the presence of cryoprotective agents to limit ice crystal formation, with subsequent fracturing of the specimen at liquid nitrogen cooled temperatures under high vacuum. The resultant fractured surface is replicated and stabilized by evaporation of carbon and platinum from an angle that confers surface three-dimensional detail to the cast. This technique has proved particularly enlightening for the investigation of cell membranes and their specializations and has contributed considerably to the understanding of cellular form to related cell function. In this report, we survey the instrument requirements and technical protocol for performing freeze-fracture, the associated nomenclature and characteristics of fracture planes, variations on the conventional procedure, and criteria for interpretation of freeze-fracture images. This technique has been widely used for ultrastructural investigation in many areas of cell biology and holds promise as an emerging imaging technique for molecular, nanotechnology, and materials science studies

In vivo actions of insulin-like growth factor-I (IGF-I) on brain myelination: studies of IGF-I and IGF binding protein-1 (IGFBP-1) transgenic mice

To study the effects and mechanisms of insulin-like growth factor I (IGF-I) on brain myelination in vivo, the morphology of myelinated axons and the expression of myelin specific protein genes have been examined in transgenic (Tg) mice that overexpress IGF-I and that those ectopically express IGF binding protein-1 (IGFBP-1), a protein that inhibits IGF-I actions when present in molar excess. Our data show that the percentage of myelinated axons and the thickness of myelin sheaths are significantly increased in IGF-I Tg and decreased in the IGFBP-1 mice. Cerebral cortical proteolipid protein (PLP) and myelin basic protein (MBP) mRNAs consistently exhibit approximately 200% increases in IGF-I Tg mice and approximately 50% decreases in IGFBP-1 Tg mice. The percentage of oligodendrocytes labeled with a PLP cRNA probe in the corpus callosum and cerebral cortex also is increased in IGF-I Tg mice and reduced in IGFBP-1 Tg mice, suggesting that IGF-I promotes oligodendrocyte survival and/or proliferation. The alterations in the number of oligodendrocytes, however, can not completely account for the changes in myelin gene expression. These results strongly indicate that IGF-I increases myelination by increasing the number of myelinated axons and the thickness of myelin sheaths, the latter by mechanisms that involve stimulation of the expression of myelin protein genes and increase of oligodendrocyte number

Guidance for researchers wanting to link NHS data using non-consent approaches:a thematic analysis of feedback from the Health Research Authority Confidentiality Advisory Group

Introduction: The use of linked data and non-consent methodologies is a rapidly growing area of health research due to the increasing detail, availability and scope of routinely collected electronic health records data. However, gaining the necessary legal and governance approvals to undertake data linkage is a complex process in England. / Objectives: We reflect on our own experience of establishing lawful basis for data linkage through Section 251 approval, with the intention to build a knowledgebase of practical advice for future applicants. / Methods: Thematic analysis was conducted on a corpus of Section 251 feedback reports from the NHS Health Research Authority Confidentiality Advisory Group. / Results: Four themes emerged from the feedback. These were: (a) Patient and Public Involvement, (b)~Establishing Rationale, (c) Data maintenance and contingency, and the need to gain (d) Further Permissions from external authorities prior to full approval. / Conclusions: Securing Section 251 approval poses ethical, practical and governance challenges. However, through a comprehensive, planned approach Section 251 approval is possible, enabling researchers to unlock the potential of linked data for the purposes of health research

Temporal structure/function variation in cultured differentiated human nasal epithelium associated with acute single exposure to tobacco smoke or E-cigarette vapor

Mucociliary clearance sustains a baseline functionality and an “on demand” capability to upregulate clearance upon irritant exposure involving mucus hypersecretion and accelerated ciliary beat frequency (CBF) modulated by nitric oxide (NO). This study characterized these elements as well as cellular and exogenous NO concentrations subsequent to a single exposure to tobacco smoke (TS) or e-cigarette vapor (EV) on cultured human airway epithelium

Nasal Nitric Oxide and Lifestyle Exposure to Tobacco Smoke

Nitric oxide (NO) is a reactive gas generated by inflammatory cells and mucosal epithelial cells of the nose and paranasal sinuses and is an important mediator in nonspecific host defense against infectious agents. However, NO also mediates physiologic events such as vasodilation, mucus hypersecretion, and mucosal disruption that are associated with inflammatory conditions, and it is a regulator of ciliary beat frequency. In the present study, we hypothesized that lifestyle exposure to tobacco smoke, whether through active smoking or by inadvertent exposure to secondhand tobacco smoke, would result in higher detectable levels of nasal NO (nNO) than are found in well-documented nonsmokers

Ultrastructural observations on cellular and subcellular aspects of experimental Mycoplasma pneumoniae disease.

The ultrastructural organization of Mycoplasma pneumoniae membranes and spatial relationships of this pathogen to epithelial cells in tracheal organ cultures were examined ultrastructurally by freeze-fracture techniques. Areas of morphologically distinct cell membrane variability characterized by membrane blebs and altered distributions of membrane associated particles were observed in replicas of M. pneumoniae cells. Inspection of the host tracheal epithelium demonstrated the alignment of M. pneumoniae to the epithelium with an accompanying deterioration in the integrity of the lumenal surface membranes and subsequent loss of the epithelial cell cytosol. Ciliary dysfunction was suggested by the observation of ciliary lesions and of disorganized epithelial cell cilia. The methodology used in these studies has permitted a new perspective of host-pathogen interactions at both the cellular and subcellular levels in tracheal organ cultures. These studies may also illustrate ultrastructural correlates of the alteration of host macromolecular synthesis in experimental M. pneumoniae infection

The Grizzly, October 6, 2016

How do UC Disability? • Ursinus\u27 Student Radio Renaissance • Get Ready to Vote, Ursinus! • Students Adjust to Philly Experience • CSCG Speaker Dissects Affordable Care Act • Schroeder Takes Love for American Studies Abroad • An Electronic Spin on Music • Opinion: Use Your Vote and Use it Wisely This November; Here\u27s What it\u27s Like to be a Republican at UC • Life of a Student Athlete at Ursinus: From Practice to Class • Kicking it Into Overdrivehttps://digitalcommons.ursinus.edu/grizzlynews/1651/thumbnail.jp

The Grizzly, October 27, 2016

How UC Students Get the News • International Students Sound Off on Election • Attorney General Candidate Discusses Platform • UC Alumni on the Campaign Trail • Local Volunteers Get Out the Vote • Opinions: The Difficulties of Voting from Past to Present; The Future of the Supreme Court is on the Ballot • UC Students Weigh in on Pro-Athlete Protests • Rein Wrestles Political Issueshttps://digitalcommons.ursinus.edu/grizzlynews/1653/thumbnail.jp