The Exact Linearization and LQR Control of Semiactive Connected Hydropneumatic Suspension System

Based on differential geometry theory, the nonlinear system of connected hydropneumatic suspension was transformed to a linear one. What is more, it realized the decoupling and inverter between the control variables and system outputs. With LQR (Linear Quadratic Regulator) control theory, a semiactive system has been developed for connected hydropneumatic suspension in this paper. By AMESim/Simulink cosimulation, the results show that the semiactive connected hydropneumatic suspension decreases the vibration of upper vehicle quickly and reduces the impact acceleration strongly both in displacement and inroll angle. Moreover, the semiactive suspension could increase the suspension dynamic deflection, which would make the system reach balance quickly and keep small vibration amplitude under the effect of disturbance

论生态经济价值观的推进

Abstract in Chinese: 传统的经济学大多建立在自然资源可无限开发利用的前提上，忽略了生态成本这一关键因素。只有摒弃以浪费资源为代价的传统经济生产方式，将生态增长视为新的社会发展衡量标准，才有可能形成稳定状态持续发展的经济。这种发展模式的转变与其说是经济变革，不如说是观念革新。新观念的关键在于如何将投资重点从对“物”的投资转向对“人”的现实的投资，其内在要求在经济、伦理、生态三个方面具体体现为知识投资、人的现实的再造以及整体的观念。Abstract in English: Most of the traditional economics based on the premise that the development and utilization of natural resources is unlimited, which neglects the environment and ecology cost. Only abandoning the traditional economic production mode at the expense of wasting resources and treating the eco growing as main measurable standard, can Steady-state Economy become possible. This kind of development model transformation is not so much economic change, as concept innovation. Which means that what matters is investment in man, not in things. The intrinsic requirements are knowledge investment, the reality of reengineering and the idea of the overall in three aspects of economic, ethical and ecological. [pp. 119-120

Divalent cations activate TRPV1 through promoting conformational change of the extracellular region

Divalent cations Mg and Ba selectively and directly potentiate transient receptor potential vanilloid type 1 heat activation by lowering the activation threshold into the room temperature range. We found that Mg potentiates channel activation only from the extracellular side; on the intracellular side, Mg inhibits channel current. By dividing the extracellularly accessible region of the channel protein into small segments and perturbing the structure of each segment with sequence replacement mutations, we observed that the S1-S2 linker, the S3-S4 linker, and the pore turret are all required for Mg potentiation. Sequence replacements at these regions substantially reduced or eliminated Mg-induced activation at room temperature while sparing capsaicin activation. Heat activation was affected by many, but not all, of these structural alternations. These observations indicate that extracellular linkers and the turret may interact with each other. Site-directed fluorescence resonance energy transfer measurements further revealed that, like heat, Mg also induces structural changes in the pore turret. Interestingly, turret movement induced by Mg precedes channel activation, suggesting that Mg-induced conformational change in the extracellular region most likely serves as the cause of channel activation instead of a coincidental or accommodating structural adjustment

Molecular Characterization of the 14-3-3 Gene Family in Brachypodium distachyon L. Reveals High Evolutionary Conservation and Diverse Responses to Abiotic Stresses

The 14-3-3 gene family identified in all eukaryotic organisms is involved in a wide range of biological processes, particularly in resistance to various abiotic stresses. Here, we performed the first comprehensive study on the molecular characterisation, phylogenetics and responses to various abiotic stresses of the 14-3-3 gene family in Brachypodium distachyon L.. A total of seven 14-3-3 genes from B. distachyon and 120 from five main lineages among 12 species were identified, which were divided into five well-conserved subfamilies. The molecular structure analysis showed that the plant 14-3-3 gene family is highly evolutionarily conserved, although certain divergence had occurred in different subfamilies. The duplication event investigation revealed that segmental duplication seemed to be the predominant form by which the 14-3-3 gene family had expanded. Moreover, seven critical amino acids were detected, which may contribute to functional divergence. Expression profiling analysis showed that BdGF14 genes were abundantly expressed in the roots, but showed low expression in the meristems. All seven BdGF14 genes showed significant expression changes under various abiotic stresses, including heavy metal, phytohormone, osmotic, and temperature stresses, which might play important roles in responses to multiple abiotic stresses mainly through participating in ABA-dependent signalling and reactive oxygen species-mediated MAPK cascade signalling pathways. In particular, BdGF14 genes generally showed upregulated expression in response to multiple stresses of high temperature, heavy metal, abscisic acid (ABA), and salicylic acid (SA), but downregulated expression under H2O2, NaCl, and polyethylene glycol (PEG) stresses. Meanwhile, dynamic transcriptional expression analysis of BdGF14 genes under longer treatments with heavy metals (Cd2+, Cr3+, Cu2+, and Zn2+) and phytohormone (ABA) and recovery revealed two main expression trends in both roots and leaves: up-down and up-down-up expression from stress treatments to recovery. This study provides new insights into the structures and functions of plant 14-3-3 genes

Polymorphisms of Gene Cassette Promoters of the Class 1 Integron in Clinical Proteus Isolates

ObjectiveTo describe the polymorphisms of gene cassette promoters of the class 1 integron in clinical Proteus isolates and their relationship with antibiotic resistance.MethodsPolymorphisms of the gene cassette promoter in 153 strains of Proteus were analyzed by PCR and nucleotide sequencing. Variable regions of atypical class 1 integrons were detected by inverse PCR and nucleotide sequencing. Enterobacterial repetitive intergenic consensus (ERIC)-PCR was used to analyze the phylogenetic relationships of class 1 integron-positive clinical Proteus isolates. Representative beta-lactamase genes (bla), including blaTEM,blaSHV,blaCTX-M-1,blaCTX-M-2,blaCTX-M-8,blaCTX-M-9,blaCTX-M-25 and blaOXA-1, and plasmid-mediated quinolone resistance (PMQR) genes including qnrA, qnrB, qnrC, qnrD, qnrS, oqxA, oqxB, qepA, and aac(6′)-Ib were also screened using PCR and sequence analysis.ResultsFifteen different gene cassette arrays and 20 different gene cassettes were detected in integron-positive strains. Of them, aadB-aadA2 (37/96) was the most common gene cassette array. Two of these gene cassette arrays (estX-psp-aadA2-cmlA1, estX-psp-aadA2-cmlA1-aadA1a-qacI-tnpA-sul3) have not previously been reported. Three different Pc-P2 variants (PcS, PcWTGN-10, PcH1) were detected among the 96 Proteus strains, with PcH1 being the most common (49/96). Strains carrying the promoters PcS or PcWTGN-10 were more resistant to sulfamethoxazole, gentamicin and tobramycin than those carrying PcH1. Strains with weak promoter (PcH1) harbored significantly more intra- and extra-integron antibiotic resistance genes than isolates with strong promoter (PcWTGN-10). Further, among 153 isolates, representative beta-lactamase genes were detected in 70 isolates (blaTEM-1, 54; blaOXA-1, 40; blaCTX-M-3, 12; blaCTX-M-14, 12; blaCTX-M-65, 5; blaCTX-M-15, 2) and representative PMQR genes were detected in 87 isolates (qnrA, 6; qnrB, 3; qnrC, 5; qnrD, 46; qnrS, 5; oqxA, 7; aac(6′)-Ib, 13; aac(6′)-Ib-cr, 32).ConclusionTo the best of our knowledge, this study provides the first evidence for polymorphisms of the class 1 integron variable promoter in clinical Proteus isolates, which generally contain relatively strong promoters. Resistance genotypes showed a higher coincidence rate with the drug-resistant phenotype in strong-promoter-containing strains, resulting in an ability to confer strong resistance to antibiotics among host bacteria and a relatively limited ability to capture gene cassettes. Moreover, strains with relatively weak integron promoters can “afford” a heavier “extra-integron antibiotic resistance gene load”. Furthermore, the gene cassettes estX, psp and the gene cassette arrays estX-psp-aadA2-cmlA1, estX-psp-aadA2-cmlA1-aadA1a-qacI-tnpA-sul3 have been confirmed for the first time in clinical Proteus isolates. Beta-lactamase genes and PMQR were investigated, and blaTEM-1 and blaOXA-1 were the most common, with qnrD and aac (6′)-Ib-cr also being dominant

Highly Efficient Production of Soluble Proteins from Insoluble Inclusion Bodies by a Two-Step-Denaturing and Refolding Method

The production of recombinant proteins in a large scale is important for protein functional and structural studies, particularly by using Escherichia coli over-expression systems; however, approximate 70% of recombinant proteins are over-expressed as insoluble inclusion bodies. Here we presented an efficient method for generating soluble proteins from inclusion bodies by using two steps of denaturation and one step of refolding. We first demonstrated the advantages of this method over a conventional procedure with one denaturation step and one refolding step using three proteins with different folding properties. The refolded proteins were found to be active using in vitro tests and a bioassay. We then tested the general applicability of this method by analyzing 88 proteins from human and other organisms, all of which were expressed as inclusion bodies. We found that about 76% of these proteins were refolded with an average of >75% yield of soluble proteins. This “two-step-denaturing and refolding” (2DR) method is simple, highly efficient and generally applicable; it can be utilized to obtain active recombinant proteins for both basic research and industrial purposes

Computational Fluid Dynamics- Based Pump Redesign to Improve Efficiency and Decrease Unsteady Radial Forces

In this study, a double volute centrifugal pump with relative low efficiency and high vibration is redesigned to improve the efficiency and reduce the unsteady radial forces with the aid of unsteady computational fluid dynamics (CFD) analysis. The concept of entropy generation rate is applied to evaluate the magnitude and distribution of the loss generation in pumps and it is proved to be a useful technique for loss identification and subsequent redesign process. The local Euler head distribution (LEHD) can represent the energy growth from the blade leading edge (LE) to its trailing edge (TE) on constant span stream surface in a viscous flow field, and the LEHD is proposed to evaluate the flow field on constant span stream surfaces from hub to shroud. To investigate the unsteady internal flow of the centrifugal pump, the unsteady Reynolds-Averaged Navier-Stokes equations (URANS) are solved with realizable k-e turbulence model using the CFD code FLUENT. The impeller is redesigned with the same outlet diameter as the baseline pump. A two-step-form LEHD is recommended to suppress flow separation and secondary flow encountered in the baseline impeller in order to improve the efficiency. The splitter blades are added to improve the hydraulic performance and to reduce unsteady radial forces. The original double volute is substituted by a newly designed single volute one. The hydraulic efficiency of the centrifugal pump based on redesigned impeller with splitter blades and newly designed single volute is about 89.2%, a 3.2% higher than the baseline pump. The pressure fluctuation in the volute is significantly reduced, and the mean and maximum values of unsteady radial force are only 30% and 26.5% of the values for the baseline pump

Machine learning-based on cytotoxic T lymphocyte evasion gene develops a novel signature to predict prognosis and immunotherapy responses for kidney renal clear cell carcinoma patients

BackgroundImmunotherapy resistance has become a difficult point in treating kidney renal clear cell carcinoma (KIRC) patients, mainly because of immune evasion. Currently, there is no effective signature to predict immunotherapy. Therefore, we use machine learning algorithms to construct a signature based on cytotoxic T lymphocyte evasion genes (CTLEGs) to predict the immunotherapy responses of patients, so as to screen patients effective for immunotherapy.MethodsIn public data sets and our in-house cohort, we used 10 machine learning algorithms to screen the optimal model with 89 combinations under the cross-validation framework, and 101 published signatures were collected. The relationship between the CTLEG signature (CTLEGS) and clinical variables was analyzed. We analyzed the role of CTLES in other types of cancer by pan-cancer analysis. The immune cell infiltration and biological characteristics were evaluated. Moreover, the response to immunotherapy and drug sensitivity of different risk groups were investigated. The key gene closely related to the signature was identified by WGCNA. We also conducted cell functional experiments and clinical tissue validation of key gene.ResultsIn public data sets and our in-house cohort, the CTLEGS shows good prediction performance. The CTLEGS can be regard as an independent risk factor for KIRC. Compared with 101 published models, our signature shows considerable superiority. The high-risk group has abundant infiltration of immunosuppressive cells and high expression of T cell depletion markers, which are characterized by immunosuppressive phenotype, minimal benefit from immunotherapy, and resistance to sunitinib and sorafenib. The CTLEGS was also strongly correlated with immunity in pan-cancer. Immunohistochemistry verified that T cell depletion marker LAG3 is highly expressed in high-risk groups in the clinical in-house cohort. The key CTLEG STAT2 can promote the proliferation, migration and invasion of KIRC cell.ConclusionsCTLEGS can accurately predict the prognosis of patients and their response to immunotherapy. It can provide guidance for the precise treatment of KIRC and help clinicians identify patients who may benefit from immunotherapy