85 research outputs found
Italian traditional tomato varieties: a focus on the Campania region
[EN] The long tradition of tomato cultivation in Italy has allowed the
evolution of a wide diversity of traditional varieties or landraces that have
been cultivated for centuries and many of them are still common in the local
markets. Adaptation to peculiar climatic and edaphic conditions, the
geographical isolation of several growing areas, the specific agro-technique,
the local gardners’ selection, also based on consumers’s sensory preferences,
have resulted in groups of landraces distributed not only by region but also
by sub-regional areas. In most cases, the cultivation of these Italian
landraces covers very limited areas being carried out in family gardens for
personal consumption and/or in small-size farms that, therefore, play a key
role in the on farm maintenance of this precious germplasm, which in many
cases is risking extinction. The Campania region has been, and still is, an
important area for tomato production with a long tradition in the selection,
cultivation and processing of the crop, resulting in a rich reservoir of
valuable tomato landraces. So far, only three Italian tomato landraces have
been granted a Protected Geographical Status under European Union law,
the PGI “Pomodoro di Pachino” form Sicily, and the two PDOs “Pomodoro
S. Marzano dell'Agro Sarnese-Nocerino” and the “Pomodorino del Piennolo
del Vesuvio” from Campania. This short review will address some of the
most popular landraces of the Campania region, with a particular focus on
the two PDOs.[ES] La larga tradición del cultivo del tomate en Italia ha permitido la evolución de una amplia diversidad de variedades tradicionales o variedades autóctonas que han sido cultivadas durante siglos, muchas de las cuales siguen siendo comunes en los mercados locales. La adaptación a las peculiares condiciones climáticas y edáficas, el aislamiento geográfico de varias zonas de cultivo, las técnicas de cultivo específicas, la selección de los agricultores locales, también basada en las preferencias sensoriales de los consumidores, han dado como resultado grupos de variedades locales distribuidas no sólo por regiones, sino por áreas sub-regionales. En la mayoría de los casos, el cultivo de estas variedades autóctonas italianas cubre áreas muy limitadas y se están llevando a cabo en huertos familiares para consumo personal y / o en fincas pequeñas que, por lo tanto, desempeñan un papel clave en el mantenimiento de este valioso germoplasma, que en muchos casos está en peligro de extinción. La región de Campania ha sido, y sigue siendo, un área importante para la producción de tomate con una larga tradición en la selección, cultivo y procesamiento de este cultivo, resultando en un rico reservorio de variedades autóctonas de tomate valiosas. Hasta el momento, sólo tres variedades autóctonas de tomate italiano han obtenido el estatus de protección geográfica conforme a la legislación de la Unión Europea, la IGP “Pomodoro di Pachino” de Sicilia y las dos DOP “Pomodoro S. Marzano dell'Agro Sarnese-Nocerino” y “Pomodorino Del Piennolo del Vesuvio" de Campania. Esta breve revisión abordará algunas de las razas tradicionales más populares de la región de Campania, con especial atención a las dos DOP.This work was supported by the European Commission Horizon 2020 program (TRADITOM grant 634561 to S.G. and P.S.).Sacco, A.; Cammareri, M.; Vitiello, A.; Palombieri, S.; Riccardi, R.; Spigno, P.; Grandillo, S. (2020). Italian traditional tomato varieties: a focus on the Campania region. En I Congrés de la Tomaca Valenciana: La Tomaca Valenciana d'El Perelló. Editorial Universitat Politècnica de València. 179-193. https://doi.org/10.4995/TOMAVAL2017.2017.6526OCS17919
Investigating physicochemical, volatile and sensory parameters playing a positive or a negative role on tomato liking
This study aimed at providing further insights into the positive and negative drivers of tomato liking. For this purpose, 13 tomato cultivars representing different typologies were characterized for physicochemical parameters and aroma volatiles, and were assessed by a trained panel for sensory descriptors, and by Italian consumers for liking. The relationships among the different parameters and their effects on consumer liking were studied by Partial Least Squares (PLS) analysis. Among physicochemical traits and sensory descriptors, seeds, reducing sugars, firmness, thick epicarp, soluble solids, sour taste, total acidity, citrate, herbaceous aroma and brightness were found to be drivers of liking, whereas pulp thickness, humidity, fruit weight, diacetyl-like odor and mealiness showed an opposite influence. For the aroma volatiles, 2-isobutylthiazole played a key role on liking and its positive contribution seemed to be supported by (Z)-3-hexen-1-ol, but suppressed by 6-methyl-5-hepten-2-ol, especially when tomatoes had a poor volatile fraction. These results represent a contribution to the knowledge that could lead to more effective breeding strategies aimed at improving tomato sensory quality. (c) 2012 Elsevier Ltd. All rights reserved
Long-Term Sphere Culture Cannot Maintain a High Ratio of Cancer Stem Cells: A Mathematical Model and Experiment
Acquiring abundant and high-purity cancer stem cells (CSCs) is an important prerequisite for CSC research. At present, researchers usually gain high-purity CSCs through flow cytometry sorting and expand them by short-term sphere culture. However, it is still uncertain whether we can amplify high-purity CSCs through long-term sphere culture. We have proposed a mathematical model using ordinary differential equations to derive the continuous variation of the CSC ratio in long-term sphere culture and estimated the model parameters based on a long-term sphere culture of MCF-7 stem cells. We found that the CSC ratio in long-term sphere culture presented as gradually decreased drift and might be stable at a lower level. Furthermore, we found that fitted model parameters could explain the main growth pattern of CSCs and differentiated cancer cells in long-term sphere culture
Targeting colorectal cancer stem cells with inducible caspase-9
Colorectal cancer stem cells (CSCs) drive tumor growth and are suggested to initiate distant metastases. Moreover, colon CSCs are reportedly more resistant to conventional chemotherapy, which is in part due to upregulation of anti-apoptotic Bcl-2 family members. To determine whether we could circumvent this apoptotic blockade, we made use of an inducible active caspase-9 (iCasp9) construct to target CSCs. Dimerization of iCasp9 with AP20187 in HCT116 colorectal cancer cells resulted in massive and rapid induction of apoptosis. In contrast to fluorouracil (5-FU)-induced apoptosis, iCasp9-induced apoptosis was independent of the mitochondrial pathway as evidenced by Bax/Bak double deficient HCT116 cells. Dimerizer treatment of colon CSCs transduced with iCasp9 (CSC-iCasp9) also rapidly induced high levels of apoptosis, while these cells were unresponsive to 5-FU in vitro. More importantly, injection of the dimerizer into mice that developed a colon CSC-iCasp9-induced tumor resulted in a strong decrease in tumor size, an increase in tumor cell apoptosis and a clear loss of CD133+ CSCs. Taken together, our data indicate that dimerization of iCasp9 circumvents the apoptosis block in CSCs, which results in effective tumor regression in vivo
The genomic landscape of cutaneous SCC reveals drivers and a novel azathioprine associated mutational signature
Cutaneous squamous cell carcinoma (cSCC) has a high tumour mutational burden (50 mutations per megabase DNA pair). Here, we combine whole-exome analyses from 40 primary cSCC tumours, comprising 20 well-differentiated and 20 moderately/poorly differentiated tumours, with accompanying clinical data from a longitudinal study of immunosuppressed and immunocompetent patients and integrate this analysis with independent gene expression studies. We identify commonly mutated genes, copy number changes and altered pathways and processes. Comparisons with tumour differentiation status suggest events which may drive disease progression. Mutational signature analysis reveals the presence of a novel signature (signature 32), whose incidence correlates with chronic exposure to the immunosuppressive drug azathioprine. Characterisation of a panel of 15 cSCC tumour-derived cell lines reveals that they accurately reflect the mutational signatures and genomic alterations of primary tumours and provide a valuable resource for the validation of tumour drivers and therapeutic targets
BAG1: The Guardian of Anti-Apoptotic Proteins in Acute Myeloid Leukemia
BCL2 associated Athano-Gene 1 (BAG1) is a multifunctional protein that has been described to be involved in different cell processes linked to cell survival. It has been reported as deregulated in diverse cancer types. Here, BAG1 protein was found highly expressed in children with acute myeloid leukemia at diagnosis, and in a cohort of leukemic cell lines. A silencing approach was used for determining BAG1's role in AML, finding that its down-regulation decreased expression of BCL2, BCL-XL, MCL1, and phospho-ERK1/2, all proteins able to sustain leukemia, without affecting the pro-apoptotic protein BAX. BAG1 down-regulation was also found to increase expression of BAG3, whose similar activity was able to compensate the loss of function of BAG1. BAG1/BAG3 co-silencing caused an enhanced cell predisposition to death in cell lines and also in primary AML cultures, affecting the same proteins. Cell death was CASPASE-3 dependent, was accompanied by PARP cleavage and documented by an increased release of pro-apoptotic molecules Smac/DIABLO and Cytochrome c. BAG1 was found to directly maintain BCL2 and to protect MCL1 from proteasomal degradation by controlling USP9X expression, which appeared to be its novel target. Finally, BAG1 was found able to affect leukemia cell fate by influencing the expression of anti-apoptotic proteins crucial for AML maintenance
TGFβ pathway limits dedifferentiation following WNT and MAPK pathway activation to suppress intestinal tumourigenesis
Recent studies have suggested increased plasticity of differentiated cells within the intestine to act both as intestinal stem cells (ISCs) and tumour-initiating cells. However, little is known of the processes that regulate this plasticity. Our previous work has shown that activating mutations of Kras or the NF-κB pathway can drive dedifferentiation of intestinal cells lacking Apc. To investigate this process further, we profiled both cells undergoing dedifferentiation in vitro and tumours generated from these cells in vivo by gene expression analysis. Remarkably, no clear differences were observed in the tumours; however, during dedifferentiation in vitro we found a marked upregulation of TGFβ signalling, a pathway commonly mutated in colorectal cancer (CRC). Genetic inactivation of TGFβ type 1 receptor (Tgfbr1/Alk5) enhanced the ability of KrasG12D/+ mutation to drive dedifferentiation and markedly accelerated tumourigenesis. Mechanistically this is associated with a marked activation of MAPK signalling. Tumourigenesis from differentiated compartments is potently inhibited by MEK inhibition. Taken together, we show that tumours arising in differentiated compartments will be exposed to different suppressive signals, for example, TGFβ and blockade of these makes tumourigenesis more efficient from this compartment
WHOLE-GENOME RE-SEQUENCING OF TWO TOMATO LANDRACES REVEALS SEQUENCE VARIATIONS UNDERPINNING KEY ECONOMICALLY IMPORTANT TRAITS
In the post-genomic era, one of the major challenges is the identification of alleles directly
responsible for phenotype variation among different genotypes within the same species. Tomato is a
model crop for understanding the development and ripening of climacteric fleshy fruits, and it is
also known to be an important source of health-promoting compounds. In addition, cultivated
tomato germplasm shows a high phenotypic variation despite its very low genetic diversity. Toward
the identification of sequence variations responsible for stress tolerance, high fruit quality and long
shelf life, we re-sequenced the genomes of two traditional landraces grown in the Campania region
(Southern Italy). Crovarese, belonging to the Corbarino type (COR), and Lucariello (LUC) are
typically grown under low water regimes and produce highly appreciated fruits, which can be stored
up to 4-8 months. We generated 65.8M and 56.4M of paired-end 30-150 bp reads with an average
insert size of 380 bp (± 52bp) and 364 bp (± 49bp) for COR and LUC, respectively. A referenceguided
assembly was performed using 'Heinz 1706' as a reference genome. We estimated a mean
coverage depth of ~15X for COR and 13X for LUC. Comparing the genomes of COR and LUC
with that of 'Heinz 1706' we found a similar distribution of SNPs (68.8% vs. 69.9%, respectively),
small deletions (8.9% vs. 8.6%) and small insertions (22.1% vs. 21.3%). Through a de novo
assembly of the unmapped reads we identified 29 and 36 new contigs in COR and LUC,
respectively. The new contigs could be assigned to the chromosomes thanks to the use of a splitread
approach. On average, the contigs inserted in COR were 654bp, whereas those inserted in LUC
were 616bp. Using custom RNA-seq data, a total of 43054 and 44576 gene loci were annotated in
COR and LUC, corresponding to 62369 and 65094 transcripts, respectively. Among the genes
showing a similar structure in COR and LUC compared to 'Heinz 1706', we identified ~2000 and
1700 SNPs causing potentially disruptive effects on the function of 1371 and 1201 genes in COR
and LUC, respectively. Interesting GO categories highly represented in genes affected by sequence
changes were identified. Major variations were present in stress-responsive genes as well as in fruit
quality and development-related genes. From a practical perspective, the identified SNPs and
InDels are candidate polymorphisms to track DNA variations associated to key traits of economic
interest
- …