545 research outputs found
Review: Applications and benefits of sexed semen in dairy and beef herds
peer-reviewedThe use of sexed semen in dairy and beef cattle production provides a number of benefits at both farm and industry levels. There is an increasing demand for dairy and beef products across the globe, which will necessitate a greater focus on improving production efficiency. In dairy farming, there is surplus production of unwanted male calves. Male dairy calves increase the risk of dystocia compared with heifer calves, and as an unwanted by-product of breeding with conventional semen, they have a low economic value. Incorporating sexed semen into the breeding programme can minimise the number of unwanted male dairy calves and reduce dystocia. Sexed semen can be used to generate herd replacements and additional heifers for herd expansion at a faster rate from within the herd, thereby minimising biosecurity risks associated with bringing in animals from different herds. Furthermore, the use of sexed semen can increase herd genetic gain compared with use of non-sorted semen. In dairy herds, a sustainable breeding strategy could combine usage of sexed semen to generate replacements only, and usage of beef semen on all dams that are not suitable for generating replacements. This results in increased genetic gain in dairy herd, increased value of beef output from the dairy herd, and reduced greenhouse gas emissions from beef. It is important to note, however, that even a small decrease in fertility of sexed semen relative to conventional semen can negate much of the economic benefit. A high fertility sexed semen product has the potential to accelerate herd expansion, minimise waste production, improve animal welfare and increase profitability compared with non-sorted conventional semen
Efficient Secretion Of Bioengineered Coagulation Factor Viii Into The Milk Of Transgenic Animals
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/106071/1/jth01670.pd
Economic impact of different strategies to use sex-sorted sperm for reproductive management in seasonal-calving, pasture-based dairy herds
peer reviewedTo maximize efficiency, profitability, and societal acceptance
of modern dairy production, it is important
to minimize the production of male dairy calves with
poor beef merit. One solution involves using sex-sorted
sperm (SS) to generate dairy replacements and breeding
all other cows to an easy-calving, short-gestation bull
with good beef merit. We used the Pasture Based Herd
Dynamic Milk Model to investigate the effect of herd
fertility and use of SS on farm net profit in a herd of
100 cows. This was completed by simulating herds with
differing fertility performance (good, average, poor),
and differing farm reproductive management [conventional
semen (CONV) or SS with varying pregnancy
per artificial insemination (P/AI) relative to CONV
(i.e., relative P/AI 100%, 85%, and 70%)]. As an additional
consideration, the method of allocating SS to
cows was also examined. The first option used SS on
random heifers and cows (S). The second option used
SS on heifers and targeted high-fertility cows (SSel). The
final option was similar to SSel, but used a fixed-time
artificial insemination (AI) protocol to facilitate AI on
the farm mating start date (SSync). For CONV, dairy
breed semen was used for AI until 50 animals were
pregnant (50% chance of a female calf), whereas for S,
SSel, or SSync the target number of animals successfully
conceiving with SS was set at 28 (based on assumed
90% chance of a female calf from pregnancies derived
from SS). Beef breed semen was used on all other dams.
The results indicated that the biggest effect on farm net
profit was not based on whether or not SS was used,
but instead was most affected by the overall fertility
performance of the herd. Total farm profit decreased
by 10% between the good and average fertility herds,
and decreased by a further 12% between the average
and poor fertility herds. In almost all situations, when
the relative P/AI with SS was 85%, use of SS led to
an overall increase of the farm net profit. There was an
economic benefit of using either SSel or SSync compared
with S for the average and poor fertility herds but not
for the good fertility herd, highlighting an interaction
between SS P/AI and overall herd fertility as well as
management practices. If the relative P/AI with SS was
<70%, the use of SS led to a decrease in profitability in
all simulations except for SSync, highlighting the importance
of a good management strategy for use of SS. The
findings in this study indicated that SS has significant
potential to help facilitate greater integration between
the dairy and beef production sectors, as well as increase
farm profitability when used appropriately
Expanding the dairy herd in pasture-based systems: The role of sexed semen within alternative breeding strategies
peer-reviewedA simulation model was developed to determine the effects of sexed semen use in heifers and lactating cows on replacement heifer numbers and rate of herd expansion in a seasonal dairy production system. Five separate artificial insemination (AI) protocols were established according to the type of semen used: (1) conventional frozen-thawed semen (CONV); (2) sexed semen in heifers and conventional semen used in cows (SS-HEIFER); (3) sexed semen in heifers and a targeted group of cows (body condition score ≥3 and calved ≥63 d), with conventional semen used in the remainder of cows (SS-CONV); (4) sexed semen in heifers and a targeted group of cows, with conventional semen in the remainder of cows for the first AI and conventional beef semen used for the second AI (SS-BEEF); or (5) sexed semen in heifers and a targeted group of cows, with conventional semen in the remainder of cows for the first AI and short gestation length semen used for the second AI (SS-SGL). Each AI protocol was assessed under 3 scenarios of sexed semen conception rate (SS-CR): 100, 94, and 87% relative to that of conventional semen. Artificial insemination was used on heifers for the first 3 wk and on cows for the first 6 wk of the 12-wk breeding season. The initial herd size was 100 cows, and all available replacement heifers were retained to facilitate herd expansion, up to a maximum herd size of 300 cows. Once maximum herd size was reached, all excess heifer calves were sold at 1 mo old. All capital expenditure associated with expansion was financed with a 15-yr loan. Each AI protocol was evaluated in terms of annual farm profit, annual cash flow, and total discounted net profit. The SS-CONV protocol generated more replacement heifers than all other AI protocols, facilitating faster expansion, and reached maximum herd size in yr 9, 9, and 10 for 100, 94, and 87% SS-CR, respectively. All AI protocols, except SS-BEEF and SS-SGL at 87% SS-CR, reached maximum herd size within the 15-yr period. Negative profit margins were experienced for SS-CONV in the first 5, 4, and 3 yr of expansion for 100, 94, and 87% SS-CR, respectively. Total discounted net profit was greater in all sexed semen AI protocols compared with CONV. This study demonstrated that, for each SS-CR, the greatest rate of expansion is achieved when using sexed and conventional semen (SS-CONV). The combined use of sexed semen and beef (SS-BEEF) or SGL (SS-SGL) semen resulted in greater discounted net profit at 100, 94, and 87% SS-CR compared with CONV, but a similar net worth change at 87% SS-CR due to a lower inventory change because SS-BEEF and SS-SGL reached maximum herd size within 15 yr
Model Independent Information On Solar Neutrino Oscillations
We present the results of a Bayesian analysis of solar neutrino data in terms
of nu_e->nu_{mu,tau} oscillations, independent from the Standard Solar Model
predictions for the solar neutrino fluxes. We show that such a model
independent analysis allows to constraint the values of the neutrino mixing
parameters in limited regions around the usual SMA, LMA, LOW and VO regions.
Furthermore, there is a strong indication in favor of large neutrino mixing and
large values of Delta m^2 (LMA region). We calculate also the allowed ranges of
the neutrino fluxes and we show that they are in good agreement with the
Standard Solar Model prediction. In particular, the ratio of the 8B flux with
its Standard Solar Model prediction is constrained in the interval [0.45,1.42]
with 99.73% probability. Finally, we show that the hypothesis of no neutrino
oscillations is strongly disfavored in a model independent way with respect to
the hypothesis of neutrino oscillations.Comment: 40 pages, 20 figures. Added references and improved figure
Effect of altering the type of dietary carbohydrate early postpartum on reproductive performance and milk production in pasture-grazed dairy cows
peer-reviewedABSTRACTThe objective of this study was to assess the effect of increasing dietary starch for approximately 30 d postpartum on reproduction outcomes in pasture-grazed, seasonal-calving dairy cows. Cows (n = 948) from 3 commercial herds were blocked by age (2, 3, and >3 yr), breed, and expected calving date and randomly assigned to 1 of 2 postpartum treatment groups: high starch (34.7 ± 1.9% nonstructural carbohydrate; mean ± SD) or low starch (22.5 ± 0.4% nonstructural carbohydrate). The high-starch group in all 3 farms received 4.0 to 4.5 kg/d of a 75:25 cracked corn:barley grain mixture in the dairy parlor, split evenly between the morning and afternoon milkings. The low-starch cows received 5.0 to 5.5 kg/d of a 50:50 mixture of palm kernel meal:soy hulls (herds 1 and 3) fed in the parlor; low-starch cows in the remaining herd (herd 2) did not receive a concentrate feed. Cows were cograzed on ryegrass–white clover dominant pastures and were offered corn silage (herds 1 and 3) and canola, corn distillers grain, and palm kernel meal (herd 1) throughout the study. At 1 mo before the start of the seasonal breeding period, the high-starch supplement was removed, and within each herd treatment groups were managed similarly through breeding. Presence of purulent vaginal discharge was assessed at 28 DIM, and tail paint was assessed weekly from 2 to 6 wk postpartum for signs of estrus. The interval to first observed estrus was unaffected by treatment (32.7 vs. 33.5 ± 2 d for high and low starch, respectively), but there were tendencies for a herd × treatment interaction for proportion of cows pregnant to first service and for pregnancy within 6 wk. This interaction was significant for the proportion of cows finally pregnant; a lower proportion of high-starch cows were pregnant to first service, pregnant by 6 wk, and pregnant by the end of the seasonal breeding period in herd 1, but diet did not affect these outcomes in the other herds. Our results do not support a positive effect on reproduction from increasing dietary starch in seasonally bred grazing dairy cows. However, the interactions indicate variability in the herd response to dietary starch early postpartum and imply that pregnancy rate could potentially be compromised through the provision of starch to grazing dairy cows in early lactation (i.e., prebreeding). The experiment was not designed to define the reasons for these interactions, but differences should be considered in future research on the subject
On the Day-Night Effect and CC to NC Event Rate Ratio Predictions for the SNO Detector
Detailed predictions for the D-N asymmetry for the Super-Kamiokande and SNO
experiments, as well as for the ratio of the CC and NC event rates measured by
SNO, in the cases of the LMA MSW and of the LOW solutions of the solar neutrino
problem, are derived. The possibilities to further constrain the regions of the
LMA MSW and LOW solutions of the solar neutrino problem by using the
forthcoming SNO data on the D-N asymmetry and on the CC to NC event rate ratio
are also discussed.Comment: 16 pages, LATEX; 10 pages of text, 12 eps-files; the text includes 6
figures; results and conclusions unchanged, the iso-(D-N) asymmetry and CC to
NC event rate ratio contour plots (Figs. 1 - 6) are given in the \Delta m^2 -
\tan^2\theta plane, a comment about the uncertainty in the theoretical
predictions for CC to NC event rate ratio in the absence of solar neutrino
oscillations and one sub-figure added; contains 3 more figures with respect
to the version to be published in Physics Letters
Characterization of the variability and repeatability of gonadotropin-releasing hormone–induced luteinizing hormone responses in dairy cows within a synchronized ovulation protocol
peer-reviewedThe primary objective was to determine the variability and repeatability of GnRH-induced LH responses. The secondary objective was to evaluate the associations among plasma LH, FSH, estradiol (E2), and progesterone (P4) concentrations. One hundred lactating Holstein cows (35 primiparous, 65 multiparous) were initially subjected to a presynchronization protocol (d 0, PGF2α; d 3, GnRH) followed 7 d later by Ovsynch (d 10, GnRH; d 17, PGF2α; 56 h later, GnRH) and timed artificial insemination 16 h after the last GnRH. Blood samples were collected immediately before the GnRH injection of presynchronization and the second GnRH of Ovsynch to determine plasma concentrations of LH, FSH, and P4. A second blood sample was collected 2 h after each of the above GnRH injections to determine GnRH-induced LH and FSH concentrations. Plasma concentrations of E2 were also determined in samples collected immediately before the second GnRH of Ovsynch. Cows that (1) had higher LH concentrations at 0 h than at 2 h after GnRH, (2) showed an ongoing spontaneous LH surge, (3) did not respond to GnRH, and (4) had P4 ≥ 0.5 ng/mL at GnRH of presynchronization and the second GnRH of Ovsynch were excluded from the analysis. The variability (coefficient of variation) and repeatability [between animal variance/(within animal variance + between animal variance)] of GnRH-induced LH response were determined from samples collected 2 h after the GnRH of presynchronization and the second GnRH of Ovsynch. The associations among plasma LH, FSH, E2, and P4 were determined at the second GnRH of Ovsynch. Mean (±SEM) LH concentrations before GnRH were 0.5 ± 0.04 and 0.6 ± 0.03 ng/mL, whereas mean LH concentrations 2 h after GnRH were 9.8 ± 1.0 and 12.1 ± 0.8 ng/mL at GnRH of presynchronization and the second GnRH of Ovsynch, respectively. The variability of GnRH-induced LH was 76.1 and 52.1% at GnRH of presynchronization and the second GnRH of Ovsynch, respectively. The repeatability estimate for GnRH-induced LH concentration between GnRH of presynchronization and Ovsynch assessments was 0.10. Plasma concentrations of LH were positively associated with FSH and E2 (r = 0.61 and 0.30, respectively) and negatively associated with P4 (r = −0.46) at the second GnRH of Ovsynch. In summary, GnRH-induced LH responses were highly variable and unrepeatable, and LH concentrations were positively associated with FSH and E2 and negatively associated with P4
Solar Neutrinos and the Principle of Equivalence
We study the proposed solution of the solar neutrino problem which requires a
flavor nondiagonal coupling of neutrinos to gravity. We adopt a
phenomenological point of view and investigate the consequences of the
hypothesis that the neutrino weak interaction eigenstates are linear
combinations of the gravitational eigenstates which have slightly different
couplings to gravity, and , , corresponding to a
difference in red-shift between electron and muon neutrinos, . We perform a analysis of the latest available solar
neutrino data and obtain the allowed regions in the space of the relevant
parameters. The existing data rule out most of the parameter space which can be
probed in solar neutrino experiments, allowing only for small values of the mixing angle () and for large mixing (). Measurements of the -neutrino energy spectrum in the SNO and
Super-Kamiokande experiments will provide stronger constraints independent of
all considerations related to solar models. We show that these measurements
will be able to exclude part of the allowed region as well as to distinguish
between conventional oscillations and oscillations due to the violation of the
equivalence principle.Comment: 20 pages + 4 figures, IASSNS-AST 94/5
Associations between postpartum phenotypes, cow factors, genetic traits, and reproductive performance in seasonal-calving, pasture-based lactating dairy cows
peer-reviewedThe objective of this study was to evaluate associations between corpus luteum (CL) status, uterine health, body condition score (BCS), metabolic status, parity, genetic merit for fertility traits, and reproductive performance in pasture-based dairy cows managed for seasonal reproduction. First- and second-lactation (n = 2,600) spring-calving dairy cows from 35 dairy farms located in Ireland were enrolled in the study. Farms were visited every 2 wk, and animals that were at wk 3 (range: 14–27 d in milk) and wk 7 (range: 42–55 d in milk) postpartum were examined. Body condition score was measured using a 1-to-5 scale in 0.25-point increments. Transrectal ultrasound examination was performed at wk 3 and 7 postpartum to determine presence or absence of CL and ultrasound reproductive tract score (scale of G1–G4). Blood samples were collected at each visit, and the concentrations of glucose, β-hydroxybutyrate (BHB), and fatty acids (FA) were analyzed using enzymatic colorimetry. Animals were grouped into 3 BCS categories [low (≤2.5), target (2.75–3.25), and high (≥3.5)], 2 CL categories (present or absent), 2 uterine health status categories (normal or abnormal), and 3 metabolic status categories [good (high glucose, low FA and BHB), poor (low glucose, high FA and BHB), and moderate (all other combinations)]. Fisher's exact test was used to test for associations between variables and was supplemented by logistic regression. More cows with a CL at wk 7 were served during the first 21 d of the breeding period compared with cows without a CL. Cows classified as having a uterine score of G3 or G4 at wk 3 and 7 had lower odds of pregnancy establishment during the breeding period compared with animals with a uterine score of G1 or G2. Animals with low BCS at wk 7 had lower odds of pregnancy establishment than cows with a target BCS. Cows classified as having good metabolic status at both wk 3 and wk 7 had greater odds of pregnancy establishment during the first 21 d of the breeding season than those classified as having poor metabolic status. Overall, primiparous cows had greater reproductive performance than second-parity cows. Animals in the quartiles with the best predicted transmitting ability for survival and calving interval had better reproductive performance compared with animals in the other quartiles. Cows that had better genetic merit for fertility traits and good metabolic status, achieved target BCS, and had a favorable ultrasound reproductive tract score and a CL present at wk 7 postpartum had superior reproductive performance
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