In vitro biological activities of potassium metaborate; antioxidative, antimicrobial and antibiofilm properties

Antioxidant, antimicrobial and antibiofilm activities of potassium metaborate (KBO2) was investigated within the present study. Antioxidant capacity of potassium metaborate was determined by β-carotene bleaching (BCB) assay and hydroxyl radical scavenging activity. Potassium metaborate was evaluated for its antimicrobial effects against selected Gram-positive bacteria, Gram-negative bacteria and a yeast via broth dilution method. The inhibition capability of potassium metaborate on the microbial biofilm formation of tested microorganisms was measured by microplate biofilm method using MTT (3- [4, 5- dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium-bromide). Biofilm inhibition capacity of potassium metaborate was also observed by Scanning Electron Microscope (SEM). Potassium metaborate was found to have the ability to scavenge hydroxyl radicals with an inhibition rate of 71.13% at 100 mM concentration. Antioxidant activity of potassium metaborate as determined by BCB assay gave higher result with an inhibition rate of 86.96% at the same concentration. According to the MIC (minimum inhibition concentration) values, the potassium metaborate inhibited the growth of C. albicans, S. aureus and E. coli at 62.5 mM concentrations while it was 31.25 mM for B. subtilis and 125 mM for P. aeruginosa. The highest antibiofilm activity was determined at the MIC of potassium metaborate with the reduction rate of 90.18% against C. albicans. It was concluded that, potassium metaborate have strong biological activities and can be effectively used for biomedical and environmental solutions

Investigations of anti-aging potential of Hypericum origanifolium Willd. for skincare formulations

WOS: 000433269500033Skin aging is caused by increased activation of extracellular matrix disruption enzymes, oxidative stress and DNA damage. Inhibition of these mechanisms by natural plants may be an encouraging approach to prevent skin aging. Hypericum is medically important plant and a source of several promising compounds. Nevertheless, there are no studies about the anti-aging potential of Hypericum origanifolium Willd (Guttiferae). The purpose of this study was to research the anti-collagenase, anti-elastase and anti-hyaluronidase effects, together with the antioxidant and genotoxic/antigenotoxic activities of H. origanifolium. Ethanol extract of H. origanifolium harvested from Turkey was tested for anti-collagenase, anti-elastase and anti-hyaluronidase activities. The extract inhibited collagenase and elastase activity. The highest ECM-degrade enzyme inhibition was demonstrated against collagenase (79.39%). The phenolic constituent and antioxidant activity were tested by Folin-Ciocalteu method (FCM), 2,2-dipenyl-1-picrylhydrazyl (DPPH) radical-scavenging and beta-carotene bleaching assay. The total phenol content revealed to be 93.4 +/- 1.6 mg GAE/g dry extract. IC50 values observed in DPPH and beta-carotene bleaching assays were 270 +/- 0.1 and 230 +/- 0.2 mu g/mL, respectively. Finally, the extract was investigated for its genotoxic/antigenotoxic effects using Ames Salmonella/microsome test system. Results indicated no sign of mutagenicity, and depending upon the concentration, the antigenotoxicity was noted with an inhibition of mutagenicity going from 21.54 to 84.90%. The results suggest that H. origanifolium can be considered as new natural sources to be potentially used as anti-aging ingredients in skin care formulations

The Research of the contents of the novel which is named Unutmaya Kimse Yok of Kemal Abdulla

Kemal Abdulla, günümüz Azerbaycan edebiyatının en güçlü temsilcilerindendir. Hikâye, roman, şiir, piyes ve çeviri alanında yetkin eserler veren yazar, çağdaş Azerbaycan edebiyatına yeni bir soluk getirmiştir. Bu çalışma, Kemal Abdulla'nın "Unutmaya Kimse Yok" isimli romanının Azerbaycan Türkçesinden Türkiye Türkçesine aktarılması ve muhteva bakımından incelenmesinden ibarettir. Kemal Abdulla'nın bahsedilen romanında çağdaş Azerbaycan edebiyatının sosyal kimliğine ve kültürel değerlerine ışık tutulmuş ve postmodernist anlayışla kurgulanan roman; romancılık geleneğine farklı bir bakış açısı kazandırmıştır.Kemal Abdulla is one of the strongest representative of Azerbaijani literature nowadays. The writer who writes stories, novels, poetry, plays and translations in the field of jurisdiction, has brought a new dimension to contemporary Azerbaijani literature. This study is consist of "Unutmaya Kimse Yok" which is named Kemal Abdullah novel is translated from Azerbaijan to Turkey Turkish and Turkish transposition and examined for content hazard. Kemal Abdullah who has mentioned in his novel of contemporary Azerbaijani literature shed light on social identity and cultural values and insights of postmodernist novels conceived; novelistic tradition has brought a different perspective

Mentha longifolia (L.) ssp. Longifolia uçucu yağı: Gıda katkı maddesi olarak doğal antioksidan ve antimutajen kaynağı

This research was performed to control the antioxidant activity, mutagenicity and antimutagenic effect of Mentha longifolia (L.) ssp. longifolia essential oil (EO), which is considered as a possible ingredient when producing healthy food. The antiradical activity was established using DPPH (2,2-diphenyl-1- picrylhydrazyl radical) and β-carotene/linoleic acid bleaching assays. The total phenolic content in the EO was evaluated by Folin Ciocalteau method (FCR). Ames Salmonella/microsome mutagenicity assay was applied to detect possible mutagenic and antimutagenic behavior. Our observations reveal that the IC50 value for DPPH radicals was 5.27 ± 0.13 mg/mL. The total antioxidant efficiency increased with an increase in the concentration of the EO, and IC50 value 11.7 ± 0.21 mg/mL. The total of phenolics was 186 ± 8.9 mg/g gallic acid equivalent/EO. Also, any concentrations of the EO used did not show mutagenic action but exhibited strong antimutagenic effects at 10.0-4.0 µg/plate concentrations. This research proposes that because of the antioxidant and antimutagenic characteristics, the EO is very advantageous and significant to the company’s manufacturing food additives.Bu araştırma sağlıklı yiyecek üretirken olası bir katkı maddesi olarak düşünülen Mentha longifolia (L.) ssp. longifolia uçucu yağının (EO) antioksidan aktivitesi, mutajenitesi ve antimutajenik etkisini kontrol etmek için gerçekleştirilmiştir. Antiradikal aktivite DPPH (2,2-diphenyl-1-picrylhydrazyl radical) yöntemi ile ve β-karoten/linoleik asit ağartma tayini kullanılarak belirlenmiştir. Uçucu yağın toplam fenolik madde içeriği Folin Ciocalteau metodu (FCR) ile tahmin edilmiştir. Olası mutajenik ve antimutajenik davranışını belirlemek için Ames Salmonella/mikrozom mutajenite testi uygulanmıştır. Gözlemlerimiz DPPH radikalleri için IC50 değerinin 5.27 ± 0.13 mg/mL olduğunu açığa çıkarmıştır. Total antioksidan etkinliği uçucu yağ konsantrasyonunun artmasıyla artmıştır ve IC50 değeri 11.7 ± 0.21 mg/mL’dir. Toplam fenolik madde içeriği 186 ± 8.9 mg/g gallik asit eşdeğeri/uçucu yağ’dır. Ayrıca uçucu yağın herhangi bir konsantrasyonu mutajenik etki göstermemiştir, fakat 10.0-4.0 µg/plate konsantrasyonlarında güçlü antimutajenik etki göstermiştir. Bu araştırma antioksidan ve antimutajenik özelliklerinden dolayı EO’nun gıda katkı maddesi üreten şirketler için çok avantajlı ve önemli olduğunu göstermektedir

The mutagenic, antimutagenic and antioxidant properties of Hypericum lydium

Context: There is a growing market demand for Hypericum sp., a pharmacologically active plant that has been traditionally used to treat various ailments. However, there have been limited studies on the extract or essential oil of Hypericum lydium Boiss (Hypericaceae). Objective: This study investigates for the first time the antioxidant, mutagenic and antimutagenic activity of an ethanol extract of H. lydium. Material and methods: Ethanol extract from aerial parts of H. lydium harvested from Turkey were tested for this mutagenic and antimutagenic activities (2.0–0.002 mg/plate) using Ames Salmonella/microsome test system. 4-Nitro-o-phenylenediamine (4-NPD) (3 μg/plate) for the Salmonella typhimurium TA98 and sodium azide (NaN3) (8 μg/plate) for the S. typhimurium TA100 were used as positive controls. The antioxidant activity, total antioxidant activity and phenolic constituent of the extract (2.0–0.002 mg/mL) was determined by the inhibition of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), β-carotene-linoleic acid model and by means of Folin–Ciocalteu reagent, respectively. Results: The extract showed no sign of mutagenicity at the tested concentrations (0.002–2.0 mg/mL), and showed concentration-dependent antimutagenic activity against NaN3 and 4-NPD ranging from 26.8 to 81.5%. The extract was found to be an efficient scavenger of DPPH (IC50 0.165 ± 0.23 mg/mL) and to inhibit β-carotene-linoleic acid bleaching (IC50 0.39 ± 0.11 mg/mL). Discussion and conclusion: These findings indicate ethanol extract of H. lydium to be a safe and effective agent that may be incorporated into new strategies for the prevention of cancer and mutagenesis

Burkholderia multivorans SB6 lipase as a detergent ingredient: characterization and stabilization

WOS: 000367541300005In this study, 265 bacterial isolates were collected from kitchen wastewater samples using Rhodamine B agar medium. Of these, 115 isolates were found to respond positively to the addition of commercial detergents. Using 16S rRNA sequence analysis, the isolate demonstrating the high stability towards commercial detergents was identified as Burkholderia multivorans. An SB6 lipase with a molecular mass of 70 kDa was purified from B. multivorans. The purified enzyme showed optimal activity at pH 9.0 and 40 A degrees C and remained stable in the presence of various metal ions, surfactants, and oxidizing agents. The addition of boron compounds improved the pH stability and thermostability of the enzyme, which displayed stability against some commercial detergents; moreover, this stability increased when boron compounds were added to the incubation medium as stabilizers. These properties make SB6 lipase an ideal choice as an additive in detergent formulations.TUBITAK, The Scientific and Technical Research Council of Turkey [TBAG-210T084]This study is a part of the Ph.D. work of Rukiye Boran. A part of this work was supported by TUBITAK, The Scientific and Technical Research Council of Turkey, through the grant number TBAG-210T084. We thank Dr. E. Sonay Elgin for help during the enzyme purification study. We would also like to express our gratitude to Dr. Omer Simsek for their kind help during the identification of bacterial strain

Production and characterization of a cold-active and n-hexane activated lipase from a newly isolated Serratia grimesii RB06-22

WOS: 000340296700004A lipase-producing bacterium isolated from raw milk was identified as Serratia grimesii based on 16S rRNA sequence analysis. The extracellular lipase was partially purified by ammonium sulfate precipitation and ultrafiltration. Maximal activity was observed at 10 degrees C, the optimum pH was 8.0 and the enzyme was stable at 5-30 degrees C for 1 h. The K-m and V-max values were 1.7 mM and 0.3 mM/min respectively. It was found that the lipase had the highest hydrolytic activity towards sunflower oil and soybean oil. CaCl2 had a stimulatory effect on lipase activity, while EDTA and iodoacetic acid slightly inhibited the lipase activity and the enzyme was strongly inhibited by PMSF. The enzyme was compatible with various non-ionic surfactants as well as sodium cholate and saponin. In addition, the enzyme was relatively stable towards oxidizing agents. This lipase exhibited maximum activity in 35% n-hexane retaining about 2191% activity for 1 h

The mutagenic, antimutagenic and antioxidant properties of Hypericum lydium

Context: There is a growing market demand for Hypericum sp., a pharmacologically active plant that has been traditionally used to treat various ailments. However, there have been limited studies on the extract or essential oil of Hypericum lydium Boiss (Hypericaceae). Objective: This study investigates for the first time the antioxidant, mutagenic and antimutagenic activity of an ethanol extract of H. lydium. Material and methods: Ethanol extract from aerial parts of H. lydium harvested from Turkey were tested for this mutagenic and antimutagenic activities (2.0-0.002 mg/plate) using Ames Salmonella/microsome test system. 4-Nitro-o-phenylenediamine (4-NPD) (3 mu g/plate) for the Salmonella typhimurium TA98 and sodium azide (NaN3) (8 mu g/plate) for the S. typhimurium TA100 were used as positive controls. The antioxidant activity, total antioxidant activity and phenolic constituent of the extract (2.0-0.002 mg/mL) was determined by the inhibition of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), beta-carotene-linoleic acid model and by means of Folin-Ciocalteu reagent, respectively. Results: The extract showed no sign of mutagenicity at the tested concentrations (0.002-2.0 mg/mL), and showed concentration-dependent antimutagenic activity against NaN3 and 4-NPD ranging from 26.8 to 81.5\%. The extract was found to be an efficient scavenger of DPPH (IC50 0.165 +/- 0.23 mg/mL) and to inhibit beta-carotene-linoleic acid bleaching (IC50 0.39 +/- 0.11 mg/mL). Discussion and conclusion: These findings indicate ethanol extract of H. lydium to be a safe and effective agent that may be incorporated into new strategies for the prevention of cancer and mutagenesis

İki doğal polisakkaritin hiyalüronidaz, kollajenaz ve elastaz inhibitör potansiyellerinin araştırılması ve antimikrobiyal, antioksidan ve homeostatik aktivitelerinin karşılaştırmalı olarak değerlendirmesi

The aim of this study was to investigate the hyaluronidase, collagenase and elastase inhibitory effects, which play important role for wound healing, together with the antibacterial, antioxidant and homeostatic activities of tragacanth gum (TG) and locust bean gum (LBG). The antimicrobial activities were tested against four bacteria and the antioxidant activities were estimated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2) radical scavenging and β-carotene bleaching assays. Homeostatic effect was evaluated with the Prothrombin Time (PT) and Activated Partial Thromboplastin Time (aPTT) test parameters. The wound healing potentials were determined with the inhibition of hyaluronidase, collagenase and elastase enzymes. The TG showed antibacterial activity against Pseudomonas aeruginosa ATCC27853 and Escherichia coli ATCC25922. The results showed that TG and LBG possessed antioxidant properties including DPPH scavenging (21.0% and 17.6%, respectively) and H2O2 radical scavenging (59.4% and 79.0%, respectively) activities. The polysaccharides displayed significantly reducing PT and aPTT results. Between the two tested polysaccharides LBG showed significant hyaluronidase and collagenase inhibition activity at 10 mg/mL concentration. These findings show that these natural polysaccharides can be used to support of wound healing.Bu çalışmanın amacı, kitre zamkı (TG) ve keçiboynuzu zamkı (LBG)'nin antibakteriyel, antioksidan ve homeostatik aktiviteleri ile birlikte yara iyileşmesi için önemli olan hiyalüronidaz, kollajenaz ve elastaz inhibitör etkilerini araştırmaktır. Antimikrobiyal aktiviteleri dört bakteriye karşı test edilerek, antioksidan aktiviteleri 1,1-difenil-2-pikrilhidrazil (DPPH), hidrojen peroksit (H202) radikal temizleme ve βkaroten ağartma deneyleri ile tespit edilmiştir. Homeostatik etki Protrombin Zamanı (PT) ve Aktive Edilen Kısmi Tromboplastin Zamanı (aPTT) test parametreleri ile değerlendirilmiştir. Yara iyileştirme potansiyelleri ise, hiyalüronidaz, kollajenaz ve elastaz inhibisyonu ile belirlenmiştir. TG, Pseudomonas aeruginosa ATCC27853 ve Escherichia coli ATCC25922'ye karşı antibakteriyel aktivite göstermiştir. Sonuçlar TG ve LBG'nin DPPH temizleme (sırasıyla %21.0 ve %17.6) ve H2O2 radikal temizleme (sırasıyla %59.4 ve %79.0) aktiviteleri de dahil olmak üzere antioksidan özelliklere sahip olduğunu göstermiştir. Polisakkaritler, PT ve aPTT'de önemli azalma göstermiştir. Test edilen iki polisakkarit arasından LBG, 10 mg/mL konsantrasyonda, önemli hiyalüronidaz ve kollajenaz inhibisyon aktivitesi göstermiştir. Bu bulgular, bu doğal polisakkaritlerin yara iyileşmesini desteklemek için kullanılabileceğini göstermektedir