Computer-based attention-demanding testing unveils severe neglect in apparently intact patients

We tested a group of ten post-acute right-hemisphere damaged patients. Patients had no neglect according to paper-and-pencil cancellation tasks. They were administered computer-based single- and dual-tasks, requiring to orally name the position of appearance (e.g. left vs. right) of briefly-presented lateralized targets. Patients omitted a consistent number of contralesional targets (approximate to 40%) under the single-task condition. When required to perform a concurrent task which recruited additional attentional resources (dual-tasks), patients' awareness for contralesional hemispace was severely affected, with less than one third of contralesional targets detected (approximate to 70% of omissions). In contrast, performance for ipsilesional (right-sided) targets was close to ceiling, showing that the deficit unveiled by computer-based testing selectively affected the contralesional hemispace. We conclude that computer-based, attention-demanding tasks are strikingly more sensitive than cancellation tasks in detecting neglect, because they are relatively immune to compensatory strategies that are often deployed by post-acute patients

Chromosome compaction and chromatin stiffness enhance diffusive loop extrusion by slip-link proteins

We use Brownian dynamics simulations to study the formation of chromatin loops through diffusive sliding of slip-link-like proteins, mimicking the behaviour of cohesin molecules. We recently proposed that diffusive sliding is sufficient to explain the extrusion of chromatin loops of hundreds of kilo-base-pairs (kbp), which may then be stabilised by interactions between cohesin and CTCF proteins. Here we show that the flexibility of the chromatin fibre strongly affects this dynamical process, and find that diffusive loop extrusion is more efficient on stiffer chromatin regions. We also show that the dynamics of loop formation are faster in confined and collapsed chromatin conformations but that this enhancement is counteracted by the increased crowding. We provide a simple theoretical argument explaining why stiffness and collapsed conformations favour diffusive extrusion. In light of the heterogeneous physical and conformational properties of eukaryotic chromatin, we suggest that our results are relevant to understand the looping and organisation of interphase chromosomes in vivo

Effect of different diets on the development, mortality, survival, food uptake and fecundity of Tupiocoris cucurbitaceus (Hemiptera: Miridae)

Several Miridae (Hemiptera) species have been identified as useful predators for biological control of the greenhouse whitefly, Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae). There is interest in determining the effects of different diets on Tupiocoris cucurbitaceus (Spinola) (Hemiptera: Miridae) in order to facilitate their breeding and use in biological control. Given that mirids can be both phytophagous and zoophytophagous, the developmental time, mortality, survival, feeding and fecundity of this species on tomato and tobacco leaves with and without the addition of T. vaporariorum or Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) eggs were studied. To determine embryonic duration, T. cucurbitaceus was allowed to oviposit on tobacco plants for 24 h at 26 °C, 83% RH and a photoperiod of 16:8 h L:D. The time of onset of the first instar nymphs was recorded. Subsequently, these nymphs fed on different diets on 9 cm × 1.5 cm plates under the same environmental conditions. Results showed that diet influences the duration of development in T. cucurbitaceus, with nymphal stadia being shorter and the adult stadium being longer when fed prey than when not fed prey. With the addition of prey, nymphal mortality was generally lower and adult survival was higher. The high consumption of T. vaporariorum eggs by T. cucurbitaceus suggests the need for subsequent studies on the latter for inclusion of this species in biological control programs in greenhouses

Predictions for surveys with the SPICA Mid-infrared Instrument

We present predictions for number counts and redshift distributions of galaxies detectable in continuum and in emission lines with the Mid-infrared (MIR) Instrument (SMI) proposed for the Space Infrared Telescope for Cosmology and Astrophysics. We have considered 24 MIR fine-structure lines, four polycyclic aromatic hydrocarbon bands (at 6.2, 7.7, 8.6 and 11.3 mu m) and two silicate bands (in emission and in absorption) at 9.7 and 18.0 mu m. Six of these lines are primarily associated with active galactic nuclei (AGNs), the others with star formation. A survey with the SMI spectrometers of 1-h integration per field of view (FoV) over an area of 1 deg(2) will yield 5 sigma detections of similar or equal to 140 AGN lines and of similar or equal to 5.2 x 10(4) star-forming galaxies, similar or equal to 1.6 x 10(4) of which will be detected in at least two lines. The combination of a shallow (20.0 deg(2), 1.4 x 10(-1) h integration per FoV) and a deep survey (6.9 x 10(-3) deg(2), 635 h integration time), with the SMI camera, for a total of similar to 1000 h, will accurately determine the MIR number counts of galaxies and of AGNs over five orders of magnitude in flux density, reaching values more than one order of magnitude fainter than the deepest Spitzer 24 mu m surveys. This will allow us to determine the cosmic star formation rate (SFR) function down to SFRs more than 100 times fainter than reached by the Herschel Observatory

Otimização do método de extração de DNA de Magnaporthe oryzae de trigo.

bitstream/item/114990/1/2014-Comunicado-tecnico-online-343.pd

The missing link of circularity in small breweries’ value chains: Unveiling strategies for waste management and biomass valorization

Despite the large quantities and possibilities of reuse of the by-products (solids and liquids) generated by the brewing industry, the proper disposal of these by-products has imposed severe problems for circular and cleaner production transitions worldwide. These challenges are still more salient for the small breweries due to the recognized lack of resources, such as knowledge, finances, and skilled staff. To address this problem, this article aims to identify sustainable strategies for waste management and biomass valorization that can be implemented in the value chain of small breweries. A mixed-method approach was implemented for the data collection and analysis to expand the evidence of the findings, including interviews with 18 small breweries and six specialists in the sector. We found that breweries mainly dispose of the by-products for animal feeding, although industry experts and the specialized literature indicate that at least 21 reuse and recycling alternatives have not been implemented in the value chain. Findings add to the literature five new alternatives informed by companies and six informed by experts for circular and cleaner production realization in small breweries' value chains. Furthermore, the article proposes a novel conceptual model to facilitate waste management and biomass valorization realization in small breweries value chains. Findings provide new insights that complement previous studies to overcome the challenges for waste management and biomass valorization in the sector. The article offers implications for theory, policymakers and managerial practice with repercussions on the production, environmental and financial issues