Вирусная реклама как средство привлечения внимания к услугам организации

ВКР посвящена изучению вирусной рекламы как средства привлечения внимания к услугам организации. Автором был разработан комплекс необходимых рекламных продуктов и мероприятий для привлечения внимания к услугам магазина детских товаров нового сегмента целевой аудитории

Crystallographic and Spectroscopic Studies of a Host–Guest Complex Consisting of a Novel Zinc Trisporphyrinate and a Chiral Monoamine

We have designed and synthesized a novel zinc trisporphyrinate with a benzene tricarboxamide as the linker. In the presence of a large excess of 1-phenylethylamine, single crystals of the corresponding 1:3 host–guest complex were obtained, which provide the crystallographic structure of a host–guest complex consisting of an achiral porphyrin and a chiral monoamine. The structure reveals the 1-phenylethylamines adopt the “inside” binding mode that is stabilized by intramolecular hydrogen bonds. The NH₂ of the 1-phenylethylamine is involved in both coordination and hydrogen bonding interactions. Circular dichroism (CD) and ultraviolet–visible spectra revealed that the 1:3 host–guest complex is dominant in the presence of a large excess of 1-phenylethylamine. The crystal structure shows there are two diastereomers of the 1:3 host–guest complexes. Density functional theory and TDDFT calculations suggest that one of the diastereomers is more energetically favorable, which dominates the CD signals

Crystallographic and Spectroscopic Studies of a Host–Guest Complex Consisting of a Novel Zinc Trisporphyrinate and a Chiral Monoamine

We have designed and synthesized a novel zinc trisporphyrinate with a benzene tricarboxamide as the linker. In the presence of a large excess of 1-phenylethylamine, single crystals of the corresponding 1:3 host–guest complex were obtained, which provide the crystallographic structure of a host–guest complex consisting of an achiral porphyrin and a chiral monoamine. The structure reveals the 1-phenylethylamines adopt the “inside” binding mode that is stabilized by intramolecular hydrogen bonds. The NH₂ of the 1-phenylethylamine is involved in both coordination and hydrogen bonding interactions. Circular dichroism (CD) and ultraviolet–visible spectra revealed that the 1:3 host–guest complex is dominant in the presence of a large excess of 1-phenylethylamine. The crystal structure shows there are two diastereomers of the 1:3 host–guest complexes. Density functional theory and TDDFT calculations suggest that one of the diastereomers is more energetically favorable, which dominates the CD signals

DataSheet_1_Diagnostic value of retinol-binding protein 4 in diabetic nephropathy: a systematic review and meta-analysis.pdf

ObjectiveDiabetic nephropathy (DN) is a major microvascular complication of diabetes and the leading cause of end-stage renal disease. Early detection and prevention of DN are important. Retinol-binding protein 4 (RBP4) has been considered as a single diagnostic marker for the detection of renal impairment. However, the results have been inconsistent. The present meta-analysis aimed to determine the diagnostic potential of RBP4 in patients in type 2 diabetes mellitus (T2DM) with DN.MethodsWe searched PubMed, Web of Science, Embase, Wanfang and CNKI databases from inception until January 2024. The meta-analysis was performed by Stata version 15.0, and sensitivity, specificity, positive and negative likelihood ratios (PLR and NLR), diagnostic odds ratio (DOR) and area under the curve (AUC) were pooled. The Quality Assessment of Diagnostic Accuracy Studies-2 tool was utilized to assess the quality of each included study. In addition, heterogeneity and publication bias were evaluated.ResultsTwenty-nine studies were included in the meta-analysis. The pooled sensitivity and specificity were 0.76 [95% confidence interval (CI), 0.71–0.80] and 0.81 (95% CI, 0.76–0.85), respectively. The results showed a pooled PLR of 4.06 (95% CI, 3.16–5.21), NLR of 0.29 (95% CI, 0.24–0.36) and DOR of 13.76 (95% CI, 9.29–20.37). The area under the summarized receiver operating characteristic curve was given a value of 0.85 (95% CI, 0.82–0.88). No obvious publication bias existed in the Deeks’ funnel plot asymmetry test.ConclusionOur findings suggest that RBP4 has a promising diagnostic value with good sensitivity and specificity for patients with T2DM with DN.</p

Stable IGF-1-GFP expression in spinal cord-derived NSCs.

<p>Phase-contrast image of pcDNA3.1-IGF-1/GFP-transfected NSCs selected by adding G418 for 14 days (A); fluorescence images of pcDNA3.1 (B) and pcDNA3.1-IGF-1-GFP transfected NSCs (C) at 24 h after transfection.</p

Effect of IGF-1 on the activation of ERK1/2.

<p>Western blot analysis of pERK1/2 in NSCs at different time points (0, 0.5, 1 or 2 h) after transfection of pcDNA3.1-IGF-1/GFP (A and B), and in IGF-1/GFP expressing NSCs treated with different concentrations of PD98059 (0, 5, 10, 20 or 40 µM) (C and D). *<i>p<</i>0.05 denotes as statistically significant in comparison to all other groups.</p

Overexpression of <i>HvHGGT</i> Enhances Tocotrienol Levels and Antioxidant Activity in Barley

Vitamin E is a potent lipid-soluble antioxidant and essential nutrient for human health. Tocotrienols are the major form of vitamin E in seeds of most monocots. It has been known that homogentisate geranylgeranyl transferase (HGGT) catalyzes the committed step of tocotrienol biosynthesis. In the present study, we generated transgenic barley overexpressing <i>HvHGGT</i> under endogenous D-Hordein promoter (<i>proHor</i>). Overexpression of <i>HvHGGT</i> increased seed size and seed weight in transgenic barley. Notably, total tocotrienol content increased by 10–15% in seeds of transgenic lines, due to the increased levels of δ-, β-, and γ-tocotrienol, but not α-tocotrienol. Total tocopherol content decreased by 14–18% in transgenic lines, compared to wild type. The antioxidant activity of seeds was determined by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azinobis­(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), and lipid peroxidation assays. Compared to wild type, radical scavenging activity of seed extracts was enhanced by 17–18% in transgenic lines. Meanwhile, the lipid peroxidation level was decreased by about 20% in transgenic barley seeds. Taken together, overexpression of <i>HvHGGT</i> enhanced the tocotrienol levels and antioxidant capacity in barley seeds

MBP expression in NSCs.

<p>Expression of MPB in NSCs transfected with pcDNA3.1 (A) and pcDNA3.1-IGF-1/GFP (B). As shown, MBP expression increased dramatically in the IGF-1-GFP expressing NSCs.</p

Effect of IGF-1 on the immediate genes of c<i>-fos,</i> c<i>-jun</i> and c<i>-myc</i> assessed by semi-quantitative RT-PCR analysis.

<p>Expressions of c<i>-fos</i>, c<i>-jun</i>, c<i>-myc</i> in IGF-1-transfected NCSs (A and B); c<i>-fos</i>, c<i>-jun</i> expression in NSCs were determined in control, or after IGF-1 expression and ERK1/2 inhibitor treatment (B and D). *<i>p<</i>0.05 denotes as statistically significant in comparison to all other groups.</p

Diethylene Glycol Serving as Ethyne Equivalent: A Sustainable Approach toward 2,3-Disubstituted Furan

In the presence of <i>tert</i>-butyl peroxide (TBHP), the copper-catalyzed annulation of 1,3-dicarbonyl compound with diethylene glycol was developed leading to 2,3-disubstituted furan. Diethylene glycol serves as a cheap and environmentally friendly equivalent of ethyne, with the release of H₂O and alcohol as clean wastes. The procedure involves a sequential <i>O</i>- and <i>C</i>- functionalization of β-ketoester by diethylene glycol