Cellular mechanisms underlying burst firing in substantia nigra dopamine neurons

Burst firing of substantia nigra dopamine (SN DA) neurons is believed to represent an important teaching signal that instructs synaptic plasticity and associative learning. However, the mechanisms through which synaptic excitation overcomes the limiting effects of somatic Ca2+-dependent K+ current to generate burst firing are controversial. Modeling studies suggest that synaptic excitation sufficiently amplifies oscillatory dendritic Ca2+ and Na+ channel currents to lead to the initiation of high-frequency firing in SN DA neuron dendrites. To test this model, visually guided compartment-specific patch-clamp recording and ion channel manipulation were applied to rodent SN DA neurons in vitro. As suggested previously, the axon of SN DA neurons was typically found to originate from a large-diameter dendrite that was proximal to the soma. However, in contrast to the predictions of the model, (1) somatic current injection generated firing that was similar in frequency and form to burst firing in vivo, (2) the efficacy of glutamatergic excitation was inversely related to the distance of excitation from the axon, (3) pharmacological blockade or genetic deletion of Ca2+ channels did not prevent high-frequency firing, (4) action potential bursts were invariably detected first at sites that were proximal to the axon, and (5) pharmacological blockade of Na+ channels in the vicinity of the axon/soma but not dendritic excitation impaired burst firing. Together, these data suggest that SN DA neurons integrate their synaptic input in a more conventional manner than was hypothesized previously

Representing older people: towards meaningful images of the user in design scenarios

Designing for older people requires the consideration of a range of difficult and sometimes highly personal design problems. Issues such as fear, loneliness, dependency, and physical decline may be difficult to observe or discuss in interviews. Pastiche scenarios and pastiche personae are techniques that employ characters to create a space for the discussion of new technological developments and as a means to explore user experience. This paper argues that the use of such characters can help to overcome restrictive notions of older people by disrupting designers' prior assumptions. In this paper, we reflect on our experiences using pastiche techniques in two separate technology design projects that sought to address the needs of older people. In the first case pastiche scenarios were developed by the designers of the system and used as discussion documents with users. In the second case, pastiche personae were used by groups of users themselves to generate scenarios which were scribed for later use by the design team. We explore how the use of fictional characters and settings can generate new ideas and undermine rhetorical devices within scenarios that attempt to fit characters to the technology, rather than vice versa. To assist in future development of pastiche techniques in designing for older people, we provide an array of fictional older characters drawn from literary and popular culture.</p

Shallow Groundwater Quality and Geochemistry in the Shields River Basin, South-Central Montana

Water samples were collected from 33 domestic wells, 2 springs, and 3 streams in the Shields River Basin (Basin) in southwest Montana. Samples were collected in 2013 to describe the chemical quality of groundwater in the Basin. Sampling was done to assess potential impacts to water quality from recent exploratory oil and gas drilling and to establish baseline water quality conditions. Wells were selected in areas near and away from oil and gas drilling and in areas susceptible to contamination. Water samples from surface water sites were collected in October to characterize base flow conditions. Physical characteristics of the land surface, soils, and shallow aquifers were used to assess groundwater susceptibility to contamination from the land surface. This analysis was completed using GIS. Samples were analyzed for major ions, trace metals, water isotopes of oxygen and hydrogen. A subset (24) of samples were analyzed for tritium and organic constituents (GRO, DRO, BTEX, methane, ethylene, and ethane). One sample exceeded the human health drinking water standard for selenium. Dissolved methane and ethylene gas were detected in six samples at concentrations less than 0.184 milligrams per liter. Three locations were resampled in 2014, and no methane or ethylene was detected. Shallow groundwater and streams are generally calcium- or sodium-bicarbonate type water with total dissolved solids concentration less than 300 milligrams per liter. Some wells produce either sodium-chloride or sodium-sulfate type water suggesting slower flow paths and more rock-water interaction. Tritium concentrations suggest that older water (TU\u3c 0.8), recharged prior to the mid-1950’s, is generally sodium type, whereas younger water (TU \u3e 4) is generally a calcium type. Water-quality data from this study were compared to available historic data in the Basin. Additionally, the USGS Produced Waters Geochemical database was queried for chemical data of produced waters from reservoir rocks throughout Montana and the surrounding states. Comparisons to historic and produced water chemical data suggest no impact to shallow groundwater quality from exploratory oil and gas drilling

Noise-induced dynamical transition in systems with symmetric absorbing states

We investigate the effect of noise strength on the macroscopic ordering dynamics of systems with symmetric absorbing states. Using an explicit stochastic microscopic model, we present evidence for a phase transition in the coarsening dynamics, from an Ising-like to a voter-like behavior, as the noise strength is increased past a nontrivial critical value. By mapping to a thermal diffusion process, we argue that the transition arises due to locally-absorbing states being entered more readily in the high-noise regime, which in turn prevents surface tension from driving the ordering process.Comment: v2 with improved introduction and figures, to appear in PRL. 4 pages, 4 figure

The Grand-Canonical Asymmetric Exclusion Process and the One-Transit Walk

The one-dimensional Asymmetric Exclusion Process (ASEP) is a paradigm for nonequilibrium dynamics, in particular driven diffusive processes. It is usually considered in a canonical ensemble in which the number of sites is fixed. We observe that the grand-canonical partition function for the ASEP is remarkably simple. It allows a simple direct derivation of the asymptotics of the canonical normalization in various phases and of the correspondence with One-Transit Walks recently observed by Brak et.al.Comment: Published versio

Dyck Paths, Motzkin Paths and Traffic Jams

It has recently been observed that the normalization of a one-dimensional out-of-equilibrium model, the Asymmetric Exclusion Process (ASEP) with random sequential dynamics, is exactly equivalent to the partition function of a two-dimensional lattice path model of one-transit walks, or equivalently Dyck paths. This explains the applicability of the Lee-Yang theory of partition function zeros to the ASEP normalization. In this paper we consider the exact solution of the parallel-update ASEP, a special case of the Nagel-Schreckenberg model for traffic flow, in which the ASEP phase transitions can be intepreted as jamming transitions, and find that Lee-Yang theory still applies. We show that the parallel-update ASEP normalization can be expressed as one of several equivalent two-dimensional lattice path problems involving weighted Dyck or Motzkin paths. We introduce the notion of thermodynamic equivalence for such paths and show that the robustness of the general form of the ASEP phase diagram under various update dynamics is a consequence of this thermodynamic equivalence.Comment: Version accepted for publicatio