Synthesis of novel heterogeneous photocatalysts based on Rose Bengal for effective wastewater disisnfection and decontamination

[EN] Anionic photosensitizers such as rose Bengal (RB) exhibit low efficiency in the photoinactivation of Gram-negative bacteria. Different strategies have been developed to improve their efficiency being the most effec-tive the addition of the photosensitizer to organic cationic materials. In this context, we have designed the synthesis of a novel heterogeneous photocatalyst based on silica in which the oxidizing properties of singlet oxygen photogenerated by RB have been combined with the chemoattraction of the bacteria to the cationic chains and their biocidal action. Thus, RB and cationic chains have been covalently linked to glass wool to obtain a photocatalyst that showed a highly efficient photodynamic activity against the Gram-positive (Enterococcus faecalis) and Gram-negative (Escherichia coli) bacteria. In addition, the new heterogeneous photocatalyst also resulted to be efficient in the decontamination of drugs such as diclofenac under visible light irradiation.We gratefully acknowledge the Universidad Complutense de Madrid, Ministerio de Universidades and recovery plan Next GenerationEU for financial support for postdoctoral contract 'Margarita Salas' for the requalification of the Spanish University System (2021-2023) , and the Spanish Ministry of Science and Innovation (PID2019-110441RB-C33 financed by MCIN/AEI/10.13039/501100011033) .Blázquez-Moraleja, A.; Moya Sanz, MDP.; Marín García, ML.; Bosca Mayans, F. (2023). Synthesis of novel heterogeneous photocatalysts based on Rose Bengal for effective wastewater disisnfection and decontamination. Catalysis Today. 413. https://doi.org/10.1016/j.cattod.2022.11.00941

What is the most effective percentage of Rose Bengal on polyamide fabrics for the visible-light photoinactivation of Gram-positive bacteria?

[EN] Heterogeneous photocatalysts based on fabric materials have achieved great relevance for water disinfection. However, studies have yet to be performed looking for the best percentage of a photosensitizer on fabric, considering the 1O2 generation efficiency as well as the charge distribution of the dye fabrics surface for bacterial inactivation. Therefore, polyamide fabrics (PAF) dyed at different Rose Bengal (RB) percentages (RB-PAF) as an anionic photosensitizer were prepared to determine the best value for photodynamic inactivation of Gram-positive bacteria (Enterococcus faecalis). Time-resolved and steady-state emission measurements, as well as laser flash photolysis experiments, were also performed. RB-PAF at a percentage of 1% (on-weigth-of-fiber, o.w. f.) showed 100% inactivation efficiency against E. faecalis (reduction of more than 6 log10 units in the viable count) in only 15 min. Contrarily, fabrics with RB values of 0.5 or 3% (o.w.f.) showed no bacterial inactivation or only a small activity (1 log10 unit reduction), respectively. Results revealed that the effects observed by RB-PAF are mediated by 3RF* and the subsequent generation of 1O2. The insignificant photooxygenation of 9,10-dimethyl anthracene observed for RB-PAF at 0.5% explains the lack of bacterial inactivation, while the low effect observed for RB-PAF at 3% is produced by electrostatic repulsions between the anionic RB and the bacteria surface. These results reveal that in order to design newly dyed fabrics for disinfection, it is important to optimize the percentage of dye to avoid aggregation of photosensitizers and to obtain an adequate net negative charge distribution in the antimicrobial fabrics.We are grateful for the CONACYT doctoral fellowship for J. F. (709358), and we also thank the Universidad Complutense de Madrid, Ministry of Universities, and Next GenerationEu recovery plan for financial support for the postdoctoral contract "Margarita Salas" for the requalification of the Spanish University System (CT31/21, 2021-2023), the Spanish Ministry of Science and Innovation (PID2019-110441RB-C33 funded by MCIN/AEI/10.13039/ 501100011033), and to the Valencian Agency for Innovation (INNEST/ 2021/75). Finally, we would also like to thank the CRUE-Universitat Politecnica de Valencia for funding the open access fee.Flores, J.; Blázquez-Moraleja, A.; Bonet-Aracil, M.; Moya Sanz, MDP.; Bosca Mayans, F.; Marín García, ML. (2023). What is the most effective percentage of Rose Bengal on polyamide fabrics for the visible-light photoinactivation of Gram-positive bacteria?. Journal of Environmental Chemical Engineering. 11(5). https://doi.org/10.1016/j.jece.2023.11063911

Efficient multi-click approach to well-defined two-faced octasilsesquioxanes: the first perfect Janus nanocube

The preparation of the first structurally well-defined Janus nanocube showing two chemically distinct opposed faces is described. The synthetic approach is based on a highly efficient and symmetrycontrolled CuAAC functionalization of an octa-azido cubic silsesquioxane with a conformationally constrained tetra-alkyne with an appropriate spatial orientation of the triple bonds.with two sets of orthogonally reactive functional groups. We gratefully acknowledge financial support by the Spanish Ministerio de Ciencia e Innovación (project MAT2010-20646- C04-03) and Ministerio de Economía y Competitividad (project MAT2014-51937-C3-1-P). We also acknowledge the Spanish Ministerio de Economía y Competitividad for a FPI contract to A. B., and CSIC for a JAEDOC contract to J. R. S. and a JAEPRE contract to M. E. P.-O., and for support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).Peer reviewe

Posfuncionalización de colorantes BODIPY para aplicaciones fotónicas y biofotónicas

El objetivo de la presente Tesis, titulada "Posfuncionalización de colorantes BODIPY para aplicaciones fotónicas y biofotónicas" es el diseño, síntesis y estudio de nuevos fluoróforos orgánicos con el fin de proporcionar soluciones químicas para el rápido desarrollo de sondas fluorescentes para aplicaciones avanzadas en biología y tecnología. Desde el punto de vista de la química orgánica, hemos basado nuestra investigación en el desarrollo de métodos fáciles, versátiles y eficientes para la posfuncionalización química del fluoróforo BODIPY (4-bora-3-a,4a-diaza-s-indaceno). El manuscrito de la tesis se puede dividir en dos partes principales. La primera parte (Capítulos 2-4) describe una estrategia general y práctica para la incorporación directa en un solo paso de α- o β-hidroxiácidos en el átomo de boro de los colorantes F-BODIPY para proporcionar los derivados O-BODIPY correspondientes..

Covalent or ionic bonding of Eosin Y to silica: New visible-light photocatalysts for redox wastewater remediation

Eosin Y (EY) is an organic dye widely used as a photoredox catalyst in organic synthesis, but there are only few studies on its use in heterogeneous photocatalysis for the degradation of aqueous Contaminants of Emerging Concern (CECs). In this context, several synthetic strategies were used to prepare heterogeneous silica-based photocatalysts where EY is anchored to glass wool (GW) covalently or using ionic bonds for obtaining GW-C-EY and GW-I-EY, respectively. The percentage of EY linked to GW-C-EY (up to 0.0034% w/w) was always lower than the obtained in GW-I-EY (0.165% w/w). Photodegradation of CECs such as noscapine (NOS), sulfamethoxazole (SMX) and ofloxacin (OFX) was studied using the new heterogeneous photocatalysts in water under visible light irradiation and results showed that only NOS is photodegraded. It was also observed that EY photoreactivity in GW-C-EY and GW-I-EY is quite similar. Interestingly, when the photocatalysts were studied using mixtures of the three CECs, a synergistic effect that favors the photodegradation of SMX and OFX was observed. Specifically, GW-I-EY achieves 100% photodegradation for NOS and ca. 50% removal of SMX and OFX. Based on photophysical and photochemical experiments, redox processes initiated by an electron transfer from NOS to the EY triplet excited state and the subsequent reaction of the generated EY with SMX or OFX justify the observed synergistic photodegradation. This finding opens up the possibility of working with complex aqueous matrices and also of smart selecting the combination of different types of contaminated waters to favor the efficiency of the photocatalytic decontamination processes.We gratefully acknowledge the Erasmus+ supports traineeships, the Universidad Complutense de Madrid, Ministerio de Universidades, and the recovery plan Next Generation EU for financial support for the postdoctoral contract “Margarita Salas” for the requalification of the Spanish University System (2021–2023), and the Spanish Ministry of Science and Innovation (PID2019-110441RB-C33 financed by MCIN/AEI/10.13039/501100011033). Finally, we would also like to thank CSIC for funding the open access fee

Compuestos y sus usos como sondas fluorescentes

Colorantes orgánicos basados en derivados de F-BODIPY y carnitina, su procedimiento de obtención y su aplicación para el etiquetado o marcaje fluorescente especifico de mitocondrias en células vivas.Peer reviewedConsejo Superior de Investigaciones Científicas (España),Fundación para el fomento en Asturias de la investigacion aplicada y la tecnología (FICYT), Fundación para la investigación e innovación biosanitaria en el principado de Asturias (FINBA)B2 Patente con examen previ

Compuestos y sus usos como sondas fluorescentes

Compuestos y sus usos como sondas fluorescentes. Colorantes orgánicos basados en derivados de F-BODIPY y carnitina, su procedimiento de obtención y su aplicación para el etiquetado o marcaje fluorescente especifico de mitocondrias en células vivas.Peer reviewedConsejo Superior de Investigaciones Científicas (España),Fundación para el fomento en Asturias de la investigacion aplicada y la tecnología (FICYT), Fundación para la investigación e innovación biosanitaria en el principado de Asturias (FINBA)A1 Solicitud de patente con informe sobre el estado de la técnic

Compounds and their uses as fluorescent probes

Organic dyes based on F-BODIPY derivatives and carnitine, method of producing same and their use for specific fluorescent labeling or tagging of mitochondria in live cellsPeer reviewedConsejo Superior de Investigaciones Científicas (España),Fundación para el fomento en Asturias de la investigacion aplicada y la tecnología (FICYT), Fundación para la investigación e innovación biosanitaria en el principado de Asturias (FINBA)A1 Solicitud de patente con informe sobre el estado de la técnic

Compuestos y sus usos como sondas fluorescentes

[EN] Organic dyes based on F -BODIPY derivatives and camitine, method for producing same and their use for the specific fluorescent labelling or tagging of mitochondria in living cells.[ES] Colorantes orgánicos basados en derivados de F-BODIPY y carnitina, su procedimiento de obtención y su aplicación para el etiquetado o mareaje fluorescente específico de mitocondrias en células vivas.Peer reviewedConsejo Superior de Investigaciones Científicas (España),Fundación para el fomento en Asturias de la investigacion aplicada y la tecnología (FICYT), Fundación para la investigación e innovación biosanitaria en el principado de Asturias (FINBA)A1 Solicitud de patente con informe sobre el estado de la técnic