Genetic code expansion for multiprotein complex engineering

We present a baculovirus-based protein engineering method that enables site-specific introduction of unique functionalities in a eukaryotic protein complex recombinantly produced in insect cells. We demonstrate the versatility of this efficient and robust protein production platform, \u2018MultiBacTATAG\u2019, (i) for the fluorescent labeling of target proteins and biologics using click chemistries, (ii) for glycoengineering of antibodies, and (iii) for structure\u2013function studies of novel eukaryotic complexes using single-molecule F\uf6rster resonance energy transfer as well as site-specific crosslinking strategies

Organic Chemistry 2011

Beuerle F, Krueger A, Lehmann M, et al. Organic Chemistry 2011. Nachrichten aus der Chemie. 2012;60(3):265-299

Organic Chemistry 2012

Beuerle F, Krueger A, Lehmann M, et al. Organic Chemistry 2012. Nachrichten aus der Chemie . 2013;61(3):265-297

Organische Chemie 2008

Braese S, Wuerthner F, Tschierske C, et al. Organische Chemie 2008. Nachrichten aus der Chemie. 2009;57(3):252-277