Didactics use of Rokytka river-basin

We live in the age of new discoveries and modern technologies and the education should not run behind the dynamic development of modern society as well. It should suit to actual trends and try to aplicate them to the education processes. In my thesis I deal with a lot of possibilities how to applicate modern technologies to the education of biology and how to use it during the pupil's home preparation. On the basis of student's attitude to the new technologies I made a material which combines the education in landscape with active multimedial preparation. Practically, I tested the colective work with this material, then I combined the gained knowledges with demostrative possibilities of the stream Rokytka. On the basis of these immediate knowledges I made a material which should lead the pupil to active searching of new information in multimedial programme and which should fix the knowledges gained in countryside

Cord blood gene expression supports that prenatal exposure to perfluoroalkyl substances causes depressed immune functionality in early childhood

<p>Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are a class of synthetic compounds that have widespread use in consumer and industrial applications. PFAS are considered environmental pollutants that have various toxic properties, including effects on the immune system. Recent human studies indicate that prenatal exposure to PFAS leads to suppressed immune responses in early childhood. In this study, data from the Norwegian BraMat cohort was used to investigate transcriptomics profiles in neonatal cord blood and their association with maternal PFAS exposure, anti-rubella antibody levels at 3 years of age and the number of common cold episodes until 3 years. Genes associated with PFAS exposure showed enrichment for immunological and developmental functions. The analyses identified a toxicogenomics profile of 52 PFAS exposure-associated genes that were in common with genes associated with rubella titers and/or common cold episodes. This gene set contains several immunomodulatory genes (<i>CYTL1, IL27</i>) as well as other immune-associated genes (e.g. <i>EMR4P, SHC4, ADORA2A</i>). In addition, this study identified <i>PPARD</i> as a PFAS toxicogenomics marker. These markers can serve as the basis for further mechanistic or epidemiological studies. This study provides a transcriptomics connection between prenatal PFAS exposure and impaired immune function in early childhood and supports current views on PPAR- and NF-<i>κ</i>B-mediated modes of action. The findings add to the available evidence that PFAS exposure is immunotoxic in humans and support regulatory policies to phase out these substances.</p