Interactive Effects of 1, 25-dihydroxyvitamin D3 and Soy Protein Extract (SPE) on Oral Cancer Growth In Vitro: Evidence for Potential Functional Relationships

ABSTRACTBackground: Previous studies have found specific soy isoflavones (Genistein, Daidzein, Glycitein) demonstrate anti-tumor properties against several cancer types, including oral cancer. Few studies have evaluated whole soy extract, containing a combination of these isoflavones and other bioreactive compounds, which may function synergistically and more effectively against oral cancers. Preliminary work by this group has now demonstrated whole soy protein extract (SPE) inhibits oral cancer cell growth specifically and selectively, through independent cell-cycle and apoptotic pathways. However, more recent evidence now suggests that ingestion of vitamin D3, either in dietary foods or supplements may potentiate the activity of soy components and their anti-tumor effects.Objective: The primary goal of this study was to investigate the interactive and inter-connected effects of 1, 25-dihydroxyvitamin D3 administration with the anti-proliferative effects of whole soy protein extract (SPE) on oral cancer and normal cell lines in vitro.Methods: Three oral squamous cell carcinoma cell lines (SCC15, SCC25, and CAL27) were treated with 1, 25-dihydroxy Vitamin D3 at physiological concentrations (10-125 nmol). Cell growth was then compared with cell treatment using soy protein extract (SPE) within the normal physiologic range (0 - 10 /L). Interactive effects were then evaluated using co-administration of SPE and 1, 25-dihydroxy Vitamin D3. Quantitative RT-PCR was performed at various time points to determine any changes in mRNA expression for key cell cycle and apoptotic signaling pathway regulators, including p53, c-myc, ornithine decarboxylase (ODC), caspase-2, caspase-8, and bax.Results: Administration of 1, 25-dihydroxy Vitamin D3 induced distinct dose-dependent, growth-inhibitory effects in all three oral cancer cell lines examined. These inhibitory effects were comparable to the overall range of growth inhibition induced by SPE. However, the combined effects of co-administration were far greater, suggesting the presence of synergistic relationships between these components. In addition, these results indicate that either treatment alone appeared to modulate mRNA expression of oral cancer cell-cycle promoters c-myc and ODC, as well as the caspase-dependent apoptosis pathway, while only 1, 25-dihydroxy Vitamin D3 administration appeared to influence the bax pathway.Conclusion: These results suggest that co-administration with 1, 25-dihydroxy Vitamin D3 and SPE may enhance their anti-tumor effects. This study may help to explain, in part, why balanced diets rich in fruits, vegetables, and soy protein, are associated with protection against development and progression of oral cancers, although further study is needed to develop specific public health recommendations for oral cancer treatment and prevention.Key words: vitamin D, soy extract, whole soy protein, oral cancer, growth inhibition

Menu engineering and dietary behavior impact on young adults’ kilocalorie choice

The obesity pandemic is associated with increased consumption of restaurant food. Labeling of menus is an intervention used to provide consumers with kilocalorie (calorie) information in hopes of them making healthier food choices. This study evaluated the relationship between young adults’ calorie choices on restaurant menus and menu design, dietary behaviors, and demographic characteristics. A 3 (fast-casual restaurants) × 4 (menu-designs based on menu engineering theories) between-subjects (n = 480, 18–24-year olds) experimental design was used. The relationship between the participants’ calorie choices (high versus low) and menu design, stage of change, gender, race, educational level and weight status was evaluated using logistic regression. All independent variables had at least one category that had greater odds (CI 95% ± 5%) of subjects choosing a lower calorie entree, except education level and race/ethnic group. Normal weight and overweight subjects had greater odds of choosing lower calorie entrees than those that were obese. In addition, subjects that had started to control their calorie intake for less than six months or had sustained this change for at least six months, had greater odds of choosing lower calorie entrees compared to others. Including a green symbol and calories on fast casual restaurant menus may influence some young adults to choose lower calorie entrees

A Study to Determine if University Athletic Teams in Desert Environments Experience Risky Diet-related Behaviours

Training and competing in desert environments may exacerbate concerns related to disordered eating, supplement use, and hydration in some student athlete populations. A survey administered equitably to both genders solicited self-reported responses from members of 18 different teams over four years from a southwestern United States university athletic program. More than 1,700 athletes responded to 42 items on the questionnaire. Teams, not individual student athletes, were the units of measure for statistical analyses. Initial analysis of results indicated that there were no overt concerns regarding dietary behaviors due to training and competing in the desert environment. Further analysis subjected the team responses to principle component factor analysis and determined the construct validity using Varimax rotation with Kaiser normalization. Six factors were identified which accounted for 93% of total variance associated with reported risky dietary related behaviors among athletic teams. Specifically, the factors contributing to dietary related behavior total variance were; 1) weight loss and exercise issues and practices -47.15%, 2) self-image - 26.00%, 3) hydration - 10.92%, 4) supplement use - 3.85%, 5) disordered eating - 3.70%, and 6) hormonal issues - 1.71%. University athletic programs should use routine assessment of dietary related behaviours, including hydration, to help identify team members practicing risky dietary related behaviours

High Resistant Starch Rice: Variation in Starch Related SNPs, and Functional, and Sensory Properties

Human diets containing greater resistant starch (RS) are associated with superior glycemic control. Although high amylose rice has higher RS (29 g/kg to 44 g/kg) than lower amylose content varieties, sensory and processing properties associated with RS have not been evaluated. This study used variants of Waxy and starch synthase II a (SSIIa) genes to divide high amylose (256 g/kg to 284 g/kg) varieties into three haplotypes to examine their effects on RS, RVA parameters, and 14 cooked rice texture properties. RVA characteristics were influenced by both genes with peak and hotpaste viscosity differentiating the three haplotypes. Setback from hotpaste viscosity was the only RVA parameter correlated with RS content across three haplotypes (r = −0.76 to −0.93). Cooked rice texture attributes were impacted more by Waxy than by SSIIa with initial starch coating, roughness, and intact particles differentiating the three haplotypes. Pairwise correlation (r = 0.46) and PCA analyses suggested that roughness was the only texture attribute associated with RS content; while protein content influenced roughness (r = 0.49) and stickiness between grains (r = 0.45). In conclusion, variation exists among genetic haplotypes with high RS for sensory traits that will appeal to diverse consumers across the globe with limited concern for negatively affecting grain processing quality

COREQ checklist for the research project PreKo, 2018-2021

For the research project “A Model for predictive assessment of cognitive workload – PreKo”,\ua0we (the researchers) have completed a COREQ (Consolidated Criteria for Reporting Qualitative\ua0Studies) checklist to transparently convey the\ua0research project’s study design in detail, with regard to the multi-case interview study that was carried\ua0out in the project

Cognitive Ergonomics of Assembly Work from a Job Demands–Resources Perspective: Three Qualitative Case Studies

In manufacturing companies, cognitive processing is required from assembly workers\ua0to perform correct and timely assembly of complex products, often with varied specifications and\ua0high quality demands. This paper explores assembly operators’ perceptions of cognitive/mental\ua0workload to provide a holistic understanding of the work conditions that affect cognitive demands\ua0and performance. While the physical loading aspects of assembly work are well known, most\ua0empirical literature dealing with cognitive/mental loading in manufacturing tends to examine a few\ua0particular aspects, rather than address the issue with a holistic system view. This semi-structured\ua0interview study, involving 50 industrial assembly operators from three Swedish companies, explores\ua0how assemblers perceive that their cognitive performance and well-being is influenced by a wide\ua0variety of factors within the context of mechanical product assembly. The interview transcripts were\ua0analysed using a priori coding, followed by bottom-up Thematic Analysis. The results indicate that a\ua0variety of systemic effects on assemblers’ cognitive performance can be classified as job demands\ua0or resources. Quite often, the absence of a resource mirrors a related demand, and “good assembly\ua0conditions”, as described by the interviewees, often re-frame demands as desirable challenges that\ua0foster motivation and positive feelings towards the work. The identified demands and resources\ua0stem from task design, timing, physical loading, intrinsic and extrinsic motivators, social teamwork\ua0and the product’s “interface” design. Despite organisational differences and conditions between the\ua0three companies that took part in the study, the results are largely consistent

Insulin clearance and the incidence of type 2 diabetes in Hispanics and African Americans: the IRAS Family Study.

ObjectiveWe aimed to identify factors that are independently associated with the metabolic clearance rate of insulin (MCRI) and to examine the association of MCRI with incident type 2 diabetes in nondiabetic Hispanics and African Americans.Research design and methodsWe investigated 1,116 participants in the Insulin Resistance Atherosclerosis Study (IRAS) Family Study with baseline examinations from 2000 to 2002 and follow-up examinations from 2005 to 2006. Insulin sensitivity (S(I)), acute insulin response (AIR), and MCRI were determined at baseline from frequently sampled intravenous glucose tolerance tests. MCRI was calculated as the ratio of the insulin dose over the incremental area under the curve of insulin. Incident diabetes was defined as fasting glucose ≥126 mg/dL or antidiabetic medication use by self-report.ResultsWe observed that S(I) and HDL cholesterol were independent positive correlates of MCRI, whereas fasting insulin, fasting glucose, subcutaneous adipose tissue, visceral adipose tissue, and AIR were independent negative correlates (all P < 0.05) at baseline. After 5 years of follow-up, 71 (6.4%) participants developed type 2 diabetes. Lower MCRI was associated with a higher risk of incident diabetes after adjusting for demographics, lifestyle factors, HDL cholesterol, indexes of obesity and adiposity, and insulin secretion (odds ratio 2.01 [95% CI 1.30-3.10], P = 0.0064, per one-SD decrease in loge-transformed MCRI).ConclusionsOur data showed that lower MCRI predicts the incidence of type 2 diabetes

The Artist as Philanthropist: Strengthening the Next Generation of Artist-Endowed Foundations, Study Report Supplement 2013

This new publication presents updated findings of the Aspen Institute National Study of Artist-Endowed Foundations, reflecting the field's continued growth since release of the Study's initial findings based on 2005 data. Drawing on new data through 2010, Study Report Supplement 2013 details this growth, analyzes factors shaping it, and explores ramifications for foundation practice. Appendices list identified foundations and present data profiles of those reporting assets of at least $1 million. New briefing papers address rising topics that will influence the field's development and a descriptive survey summarizes estate planning literature as it pertains to visual artists