Actinomicetos termoalcalófilos del área subtropical de Jujuy, Argentina

El objetivo de este trabajo fue examinar los actinomicetos termoalcalófilos presentes en el área subtropical de Jujuy, Argentina, caracterizada por el cultivo de la caña de azúcar. Se aislaron en medio con novobiocina las especies Laceyella putida, Laceyella sacchari, Thermoactinomyces intermedius, Thermoactinomyces vulgaris y Thermoflavimicrobium dichotomicum a partir de la rizósfera de plantas y de renuevos de caña de azúcar, así como de suelos de pastura y de monte natural. El pH de los suelos era casi neutro a ligeramente alcalino, excepto en un solo caso en que el suelo estaba acidificado por licor láctico. El número de actinomicetos encontrados sobre los tejidos vivos y en el bagazo recién obtenido o almacenado según el método de Ritter fue pequeño en comparación con el observado sobre las hojas en descomposición. L. sacchari predominó respecto de T. vulgaris. Se aislaron especies termoalcalófilas de Laceyella, Thermoactinomyces, Thermoflavimicrobium, Saccharomonospora, Streptomyces y Thermononospora de los residuos compostados de caña de azúcar utilizando medio sin novobiocina. El aire capturado cerca de pilas de bagazo en compostaje contenía esporos de estos organismos.The objective of this study was to examine the alkalithermophilic actinomycete communities in the subtropical environment of Jujuy, Argentina, characterized by sugarcane crops. Laceyella putida, Laceyella sacchari, Thermoactinomyces intermedius, Thermoactinomyces vulgaris and Thermoflavimicrobium dichotomicum were isolated on the media with novobiocin, from sugar cane plants and renewal rhizospheres, and grass and wood soils. Soil pH was almost neutral or lightly alkaline, except for grass soil acidified by lactic liquor. A smaller number of actinomycetes was found on the living plants and bagasse (recently obtained or stored according to the Ritter method) with respect to decomposed leaves on the soil. Thermophilic species of Laceyella, Thermoactinomyces, Thermoflavimicrobium, Saccharomonospora, Streptomyces and Thermononospora were isolated on the media without novobiocin, from composted sugar cane residues. Air captured near composted bagasse piles, contained alkalithermophilic actinomycete spores.Fil: Carrillo, L.. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias; ArgentinaFil: Benitez Ahrendts, Marcelo Rafael. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias; ArgentinaFil: Maldonado, Marcos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Jujuy. Facultad de Ciencias Agrarias; Argentin

Análisis de fluctuación térmica y perspectivas en vestimenta inteligente

La monitorización de parámetros biológicos es cada día más empleada en el ámbito médico, deportivo (atletismo, ciclismo, montañismo) y profesional. En este trabajo presentamos un estudio realizado, empleando tarjetas de adquisición de temperatura TURBOTAG, que almacenan hasta 700 datos que se pueden trasferir al PC a través de un lector RF. Estas tarjetas se facilitaron a 15 alumnos de doctorado y las mantuvieron pegadas a la piel o en el bolsillo de alguna de sus prendas, durante 3 días. Tras analizar los datos obtenidos, se puede observar que la temperatura tiene oscilaciones ocasionadas por los cambios externos de temperatura, pero en ocasiones esas fluctuaciones pueden producirse por variaciones anómalas de la temperatura corporal. El concepto de traje inteligente surge ante la posibilidad de englobar la sensórica apropiada y la tecnología capaz de procesar la señal, acondicionarla y almacenarla o transmitirla de forma inalámbrica a un PC o una PDA, y además emplear nuevos textiles especialmente diseñados

Determination of suitable irrigation lengths and intervals using capacitance humidity sensors for a sandy soil

production, during the summer of 2010. This farm is integrated at the Spanish research network for the sugar beet development (AIMCRA) which regarding irrigation, focuses on maximizing water saving and cost reduction. According to AIMCRA 0 s perspective for promoting irrigation best practices, it is essential to understand soil response to irrigation i.e. maximum irrigation length for each soil infiltration capacity. The Use of Humidity Sensors provides foundations to address soil 0 s behavior at the irrigation events and, therefore, to establish the boundaries regarding irrigation length and irrigation interval. In order to understand to what extent farmer 0 s performance at Tordesillas farm could have been potentially improved, this study aims to address suitable irrigation length and intervals for the given soil properties and evapotranspiration rates. In this sense, several humidity sensors were installed: (1) A Frequency Domain Reflectometry (FDR) EnviroScan Probe taking readings at 10, 20, 40 and 60cm depth and (2) different Time Domain Reflectometry (TDR) Echo 2 and Cr200 probes buried in a 50cm x 30cm x 50cm pit and placed along the walls at 10, 20, 30 and 40 cm depth. Moreover, in order to define soil properties, a textural analysis at the Tordesillas Farm was conducted. Also, data from the Tordesillas meteorological station was utilized

Contemporary Micro-IT Capabilities and Organizational Performance: The Role of Online Customer Engagement

We theorize that the development of two contemporary social commerce-IT capabilities (social media and e-business technology) help to online engage customers to improve organizational performance. We test this theory by employing a secondary dataset on a sample of 100 small U.S. firms. The empirical analysis suggests that social media capability and e-business technology capability positively affect organizational performance through social and conventional online customer engagement. Research and managerial implications are discussed

Molecular signature of response and potential pathways related to resistance to the HSP90 inhibitor, 17AAG, in breast cancer

<p>Abstract</p> <p>Background</p> <p>HSP90 may be a favorable target for investigational therapy in breast cancer. In fact, the HSP90 inhibitor, 17AAG, currently has entered in phase II clinical trials as an anticancer agent in breast and other tumors. Since HSP90 inhibition leads to global depletion of oncogenic proteins involved in multiple pathways we applied global analysis using gene array technology to study new genes and pathways involved in the drug response in breast cancer.</p> <p>Methods</p> <p>Gene expression profiling using Whole Human Genome Agilent array technology was applied to a total of six sensitive and two resistant breast cancer cell lines pre-treatment and treated with the 17AAG for 24 and 48 hours.</p> <p>Results</p> <p>We have identified a common molecular signature of response to 17AAG composed of 35 genes which include novel pharmacodynamic markers of this drug. In addition, different patterns of HSP90 client transcriptional changes after 17AAG were identified associated to the sensitive cell lines, which could be useful to evaluate drug effectiveness. Finally, we have found differentially expressed pathways associated to resistance to 17AAG. We observed significant activation of NF-κB and MAPK pathways in resistant cells upon treatment, indicating that these pathways could be potentially targeted to overcome resistance.</p> <p>Conclusions</p> <p>Our study shows that global mRNA expression analysis is a useful strategy to examine molecular effects of drugs, which allowed us the discovery of new biomarkers of 17AAG activity and provided more insights into the complex mechanism of 17AAG resistance.</p

The relationship between IT infrastructure leveraging, talent management and operational sustainability, and their effects on the business value of the operations strategy

The purpose of this research is to examine the role of information technology (IT) in the adoption of human resources (HR) and environmental management activities, a research topic that has not received much attention in prior literature. Drawing on the theory of operational and dynamic capabilities, we propose a model in which IT infrastructure leveraging enables the firm to create value from its operations management both directly and indirectly through talent management and operational sustainability. We test the model using the structural equation modeling (SEM) technique with an innovative secondary data set collected for a sample of 63 large firms. The empirical analysis suggests that IT infrastructure leveraging enables the firm’s proficiency to manage talent, which in turn facilitates execution of a more sustainable operations strategy to increase firm performance. The analysis also shows that IT infrastructure leveraging impacts firm performance directly in the long-term

A large strain anisotropic elastoplastic continuum theory for nonlinear kinematic hardening and texture evolution

In this paper we present a continuum theory for large strain anisotropic elastoplasticity based on a decomposition of the modified plastic velocity gradient into energetic and dissipative parts. The theory includes the Armstrong and Frederick hardening rule as well as multilayer models as special cases even for large strain anisotropic elastoplasticity. Texture evolution may also be modelled by the formulation, which allows for a meaningful interpretation of the terms of the dissipation equatio

Subtle sexual plumage color dimorphism and size dimorphism in a South American colonial breeder, the Monk Parakeet (Myiopsitta monachus)

Parrots (Psittacidae Family) are one of the most colorful groups of birds in the world, their colors produced both structurally and via unusual pigments (psittacofulvins). Most species are considered to be monogamous, and many have been viewed historically as sexually monomorphic and monochromatic. However, studies using morphometric analysis and spectrophotometric techniques have revealed sexual size dimorphism and also sexual plumage color dimorphism among some species. The Monk Parakeet (Myiopsitta monachus), a native parrot of South America, is an interesting species for the study of plumage coloration and size since it is considered sexually monochromatic and monomorphic. Furthermore, recent studies show that the Monk Parakeet has extra-bond paternity behavior and even breeding trios, which suggests that sexual selection may play an important role in this species, and that it might have sexually dimorphic plumage (albeit imperceptible by humans) and be dimorphic in size. Methods: For the determination of plumage color we used spectrophotometry in the range of avian vision (300-700 nm) and performed a morphological analysis. Results: Our spectrophotometric results indicate that the Monk Parakeet shows subtle sexual plumage color dimorphism in three (crown, nape and wing) out of twelve body regions. Similarly, our morphometric analysis showed that there are subtle sex differences in body size (bill and weight). Conclusions: Although the Monk Parakeet shows extra-bond paternity and breeding trio behaviors which could increase sexual dimorphism, these behaviors occur among highly related individuals; perhaps the high rate of inbreeding is responsible for the attenuation of sexual plumage color dimorphism and sex differences in body size observed.Fil: Morales, Macarena. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Centro de Zoología Aplicada; ArgentinaFil: Gigena, Deysi J.. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Benitez-Vieyra, Santiago Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Valdez, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Diversidad y Ecología Animal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto de Diversidad y Ecología Animal; Argentin

Evaluation of two protocols developed for Xanthomonas albilineas molecular detection in sugarcane

Leaf scald disease caused by Xanthomonas albilineans (Ashby) Dowson is one of the most devastating diseases of sugarcane which has a direct impact on production and manufacturing quality. Currently, molecular tests have been proposed for the detection of this phytopathogen. In this work, two PCR's protocols developed by Pan et al. in 1997 and 1999 were evaluated, with primers that amplify a fragment of the ITS region between the ribosomal genes 16S and 23S. The search of X. albilineans was done from the juice obtained of 35 asymptomatic and symptomatic plants of sugarcane cultivated in localities of the provinces of Misiones and Corrientes, Argentina. X. albilineans was detected in only one of the symptomatic samples but in any of the asymptomatic ones. The protocol developed in 1999 was the one that gave the best results. It is important to emphasize the detection of this phytopathogen in the province of Misiones.Fil: Arriola Benitez, Paula Constanza. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Padovan, C.. Programa de Desarrollo de Áreas Rurales; ArgentinaFil: Quiroga, Marina Inés. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentin

A century of Shope Papillomavirus in museum rabbit specimens

Sylvilagus floridanus Papillomavirus (SfPV) causes growth of large horn-like tumors on rabbits. SfPV was described in cottontail rabbits (probably Sylvilagus floridanus) from Kansa and Iowa by Richard Shope in 1933, and detected in S. audubonii in 2011. It is known almost exclusively from the US Midwest. We explored the University of Kansas Natural History Museum for historical museum specimens infected with SfPV, using molecular techniques, to assess if additional wild species host SfPV, and whether SfPV occurs throughout the host range, or just in the Midwest. Secondary aims were to detect distinct strains, and evidence for strain spatio-temporal specificity. We found 20 of 1395 rabbits in the KU collection SfPV symptomatic. Three of 17 lagomorph species (S. nuttallii, and the two known hosts) were symptomatic, while Brachylagus, Lepus and eight additional Sylvilagus species were not. 13 symptomatic individuals were positive by molecular testing, including the first S. nuttallii detection. Prevalence of symptomatic individuals was significantly higher in Sylvilagus (1.8%) than Lepus. Half of these specimens came from Kansas, though new molecular detections were obtained from Jalisco—Mexico’s first—and Nebraska, Nevada, New Mexico, and Texas, USA. We document the oldest lab-confirmed case (Kansas, 1915), predating Shope’s first case. SfPV amplification was possible from 63.2% of symptomatic museum specimens. Using multiple methodologies, rolling circle amplification and, multiple isothermal displacement amplification in addition to PCR, greatly improved detection rates. Short sequences were obtained from six individuals for two genes. L1 gene sequences were identical to all previously detected sequences; E7 gene sequences, were more variable, yielding five distinct SfPV1 strains that differing by less than 2% from strains circulating in the Midwest and Mexico, between 1915 and 2005. Our results do not clarify whether strains are host species specific, though they are consistent with SfPV specificity to genus Sylvilagus.Costs of lab work were funded by the Spanish Ministry of Science and Innovation, (Ref CGL2010-15734/BOS) awarded to JPT. RW was supported by the Programa Internacional de Captación de Talento (PICATA) de Moncloa Campus de Excelencia Internacional(http://www.campusmoncloa.es/en/calls/picata.php) while writing the manuscript. The authors thank The One University Open Access Author Fund at The University of Kansas for funding this publication. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript