28 research outputs found
New insight into dolphin morbillivirus phylogeny and epidemiology in the northeast Atlantic: opportunistic study in cetaceans stranded along the Portuguese and Galician coasts
Sometimes Sperm Whales (Physeter macrocephalus) Cannot Find Their Way Back to the High Seas: A Multidisciplinary Study on a Mass Stranding
BACKGROUND: Mass strandings of sperm whales (Physeter macrocephalus) remain peculiar and rather unexplained events, which rarely occur in the Mediterranean Sea. Solar cycles and related changes in the geomagnetic field, variations in water temperature and weather conditions, coast geographical features and human activities have been proposed as possible causes. In December 2009, a pod of seven male sperm whales stranded along the Adriatic coast of Southern Italy. This is the sixth instance from 1555 in this basin. METHODOLOGY/PRINCIPAL FINDINGS: Complete necropsies were performed on three whales whose bodies were in good condition, carrying out on sampled tissues histopathology, virology, bacteriology, parasitology, and screening of veins looking for gas emboli. Furthermore, samples for age determination, genetic studies, gastric content evaluation, stable isotopes and toxicology were taken from all the seven specimens. The animals were part of the same group and determined by genetic and photo-identification to be part of the Mediterranean population. Causes of death did not include biological agents, or the "gas and fat embolic syndrome", associated with direct sonar exposure. Environmental pollutant tissue concentrations were relatively high, in particular organochlorinated xenobiotics. Gastric content and morphologic tissue examinations showed a prolonged starvation, which likely caused, at its turn, the mobilization of lipophilic contaminants from the adipose tissue. Chemical compounds subsequently entered the blood circulation and may have impaired immune and nervous functions. CONCLUSIONS/SIGNIFICANCE: A multi-factorial cause underlying this sperm whales' mass stranding is proposed herein based upon the results of postmortem investigations as well as of the detailed analyses of the geographical and historical background. The seven sperm whales took the same "wrong way" into the Adriatic Sea, a potentially dangerous trap for Mediterranean sperm whales. Seismic surveys should be also regarded as potential co-factors, even if no evidence of direct impact has been detected
The structural history of the Mont Blanc massif with regard to models for its recent exhumation
First molecular determination of herpesvirus from two mysticete species stranded in the Mediterranean Sea
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Genetic comparison among dolphin morbillivirus in the 1990-1992 and 2006-2008 Mediterranean outbreaks
In 1990, dolphin morbillivirus (DMV) killed thousands of striped dolphins in the Mediterranean. Subsequently, the prevalence of the infection declined in this species. In 2006-2008, the virus killed not only numerous striped dolphins but also long-finned pilot whales. All partial sequences of the phosphoprotein and nucleoprotein genes obtained thus far from different host species during the 2006-2008 outbreak show 100% identity, suggesting that a single virus was involved, and these sequences are nearly identical to the 1990 Spanish strain. Here our first objective was to determine the sequence identity between the morbillivirus from the 2006-2008 outbreak and the 1990 Spanish strain by sequencing more extensive genomic regions of strains from one pilot whale and one striped dolphin stranded in 2007. The second objective was to investigate the relationship between the 1990 and 2007 strains by constructing a phylogenetic tree based on the phosphoprotein gene to compare several Cetacean morbilliviruses, and another tree based on the nearly complete genomes of Mediterranean DMV. The third objective was to identify the most variable regions in the DMV genomes.Results showed that the two 2007 Spanish strains were 99.9% identical over 9050. bp and should be considered the same virus, and that this virus is 99.3-99.4% similar to the 1990 Spanish strain. The phylogenetic trees, together with the common geographical area for the two outbreaks, suggest that the 2007 DMV strains evolved from the 1990 DMV strain. Pilot whales do not seem to have been exposed or infected during the 1990-1992 epidemic, since these populations appeared to be immunologically naïve in 2006-2008. Our results suggest that the virus may have evolved in striped dolphin populations prior to the 2006-2008 outbreak, after which it entered the long-finned pilot whale, perhaps aided by an alanine to valine mutation in the N-terminal domain of the fusion protein. © 2011 Elsevier B.V
Phylogenetic analysis of a new Cetacean morbillivirus from a short-finned pilot whale stranded in the Canary Islands
Cetacean morbillivirus (CeMV) is considered the most pathogenic virus in cetaceans. Three strains have been already described the dolphin morbillivirus (DMV), the porpoise morbillivirus (PMV) and the tentatively named pilot whale morbillivirus (PWMV).This study describes the molecular characterization of a strain of CeMV detected in the brain of a short-finned pilot whale that had stranded in the Eastern Atlantic Ocean around the Canary Islands and that showed lesions compatible with morbilliviral disease. Sequences for the nucleoprotein, phosphoprotein, fusion protein and haemagglutinin genes were obtained. The phylogenetic study showed high homology (97%) with the PWMV strain previously detected from a long-finned pilot whale stranded in the Western Atlantic Ocean. These results support the existing classification of CeMV into three principal genetic clusters. © 2010 Elsevier Ltd
Presence of herpesvirus in striped dolphins stranded during the cetacean morbillivirus epizootic along the Mediterranean Spanish coast in 2007
A screening for herpesvirus (HV) was carried out using a tissue bank obtained from the cetacean morbillivirus (CeMV) mortality episode that occurred along the Mediterranean Spanish coast in 2007. A total of 14 cetaceans, including six long-finned pilot whales and eight striped dolphins, were studied using histopathology and molecular analysis to detect HV and CeMV. In five of the eight dolphins (62.5%) infected with CeMV, eight novel HV sequences were also detected. No HV lesions were found in any of the coinfected dolphins, which may indicate that HV did not contribute to the mortality in the CeMV outbreak. This is the first report of HV infection in any cetacean from the Mediterranean Sea. © 2010 Springer-Verlag
Oxidative dehydrogenation of ethyl lactate to ethyl pyruvate over vanadium and iron antimonates catalysts
International audienceThe oxidative dehydrogenation of ethyl lactate to ethyl pyruvate, corresponding to the first step of a new process in the industrial production of methionine, has been investigated. Iron and vanadium antimonates were developed as catalysts, and were optimized to reach 87% conversion of ethyl lactate, with 88% selectivity to ethyl pyruvate, at only 275 °C. The catalysts were characterized before and after catalytic testing, and in situ using various techniques, including X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and XANES spectroscopy. The results show that neither the Sb 3+ /Sb 5+ nor the Fe 2+ /Fe 3+ redox couple were involved in the dehydrogenation of ethyl lactate, or in the catalysts re-oxidation. The active and selective catalytic sites correspond to surface V 5+ species. These species should not be considered as part of the bulk oxide, but as supra-surface species whose surface content is monitored with the bulk composition
Diagnosis of Cetacean morbillivirus A sensitive one step real time RT fast-PCR method based on SYBR® Green
Cetacean morbillivirus (CeMV) (family Paramyxoviridae, genus Morbillivirus) is considered the most pathogenic virus of cetaceans. It was first implicated in the bottlenose dolphin (Tursiops truncatus) mass stranding episode along the Northwestern Atlantic coast in the late 1980s, and in several more recent worldwide epizootics in different Odontoceti species. This study describes a new one step real-time reverse transcription fast polymerase chain reaction (real-time RT-fast PCR) method based on SYBR® Green to detect a fragment of the CeMV fusion protein gene. This primer set also works for conventional RT-PCR diagnosis. This method detected and identified all three well-characterized strains of CeMV porpoise morbillivirus (PMV), dolphin morbillivirus (DMV) and pilot whale morbillivirus (PWMV). Relative sensitivity was measured by comparing the results obtained from 10-fold dilution series of PMV and DMV positive controls and a PWMV field sample, to those obtained by the previously described conventional phosphoprotein gene based RT-PCR method. Both the conventional and real-time RT-PCR methods involving the fusion protein gene were 100- to 1000-fold more sensitive than the previously described conventional RT-PCR method. © 2015 Elsevier B.V