On the Equivalence of Geometric and Analytic K-Homology

We give a proof that the geometric K-homology theory for finite CW-complexes defined by Baum and Douglas is isomorphic to Kasparov's K-homology. The proof is a simplification of more elaborate arguments which deal with the geometric formulation of equivariant K-homology theory.Comment: 29 pages, v4: corrected definition of E in proof of Prop 3.

Horizontal gene transfer of acetyltransferases, invertases and chorismate mutases from different bacteria to diverse recipients

Background: Hoplolaimina plant-parasitic nematodes (PPN) are a lineage of animals with many documented cases of horizontal gene transfer (HGT). In a recent study, we reported on three likely HGT candidate genes in the soybean cyst nematode Heterodera glycines, all of which encode secreted candidate effectors with putative functions in the host plant. Hg-GLAND1 is a putative GCN5-related N-acetyltransferase (GNAT), Hg-GLAND13 is a putative invertase (INV), and Hg-GLAND16 is a putative chorismate mutase (CM), and blastp searches of the non- redundant database resulted in highest similarity to bacterial sequences. Here, we searched nematode and non- nematode sequence databases to identify all the nematodes possible that contain these three genes, and to formulate hypotheses about when they most likely appeared in the phylum Nematoda. We then performed phylogenetic analyses combined with model selection tests of alternative models of sequence evolution to determine whether these genes were horizontally acquired from bacteria. Results: Mining of nematode sequence databases determined that GNATs appeared in Hoplolaimina PPN late in evolution, while both INVs and CMs appeared before the radiation of the Hoplolaimina suborder. Also, Hoplolaimina GNATs, INVs and CMs formed well-supported clusters with different rhizosphere bacteria in the phylogenetic trees, and the model selection tests greatly supported models of HGT over descent via common ancestry. Surprisingly, the phylogenetic trees also revealed additional, well-supported clusters of bacterial GNATs, INVs and CMs with diverse eukaryotes and archaea. There were at least eleven and eight well-supported clusters of GNATs and INVs, respectively, from different bacteria with diverse eukaryotes and archaea. Though less frequent, CMs from different bacteria formed supported clusters with multiple different eukaryotes. Moreover, almost all individual clusters containing bacteria and eukaryotes or archaea contained species that inhabit very similar niches. Conclusions: GNATs were horizontally acquired late in Hoplolaimina PPN evolution from bacteria most similar to the saprophytic and plant-pathogenic actinomycetes. INVs and CMs were horizontally acquired from bacteria most similar to rhizobacteria and Burkholderia soil bacteria, respectively, before the radiation of Hoplolaimina. Also, these three gene groups appear to have been frequent subjects of HGT from different bacteria to numerous, diverse lineages of eukaryotes and archaea, which suggests that these genes may confer important evolutionary advantages to many taxa. In the case of Hoplolaimina PPN, this advantage likely was an improved ability to parasitize plants

Manipulation of Plant Cells by Cyst and Root-Knot Nematode Effectors

A key feature of sedentary plant-parasitic nematodes is the release of effector proteins from their esophageal gland cells through their stylets into host roots. These proteinaceous stylet secretions have been shown to be crucial for successful parasitism by mediating the transition of normal root cells into specialized feeding sites and by negating plant defenses. Recent technical advances of purifying mRNA from esophageal gland cells of plant-parasitic nematodes coupled with emerging sequencing technologies is steadily expanding our knowledge of nematode effector repertoires. Host targets and biological activities of a number of nematode effectors are continuously being reported and, by now, a first picture of the complexity of sedentary nematode parasitism at the molecular level is starting to take shape. In this review, we highlight effector mechanisms that recently have been uncovered by studying the host–pathogen interaction. These mechanisms range from mediating susceptibility of host plants to the actual triggering of defense responses. In particular, we portray and discuss the mechanisms by which nematode effectors modify plant cell walls, negate host defense responses, alter auxin and polyamine signaling, mimic plant molecules, regulate stress signaling, and activate hypersensitive responses. Continuous molecular characterization of newly discovered nematode effectors will be needed to determine how these effectors orchestrate host signaling pathways and biological processes leading to successful parasitism

“Cyst-ained” research into Heterodera parasitism

Nematodes are roundworms that constitute the phylum Nematoda. Only a small fraction of nematode genera contains plant-parasitic or animal-parasitic species, while the majority of nematodes are free-living [1]. Heterodera glycines, the soybean cyst nematode, is a plant-parasitic nematode causing major damage to soybean production worldwide. Annual United States yield loss estimates due to H. glycines range up to $1.2 billion, likely making this nematode the most serious pathogen threat to sustainable soybean production [2]. While cyst nematode-resistant soybean cultivars are available, they do not control all H. glycines biotypes present in a given field and, therefore, select for virulent nematode populations that can overcome available resistance genes, leading to a slow but steady erosion of resistance efficacy [3]. Clearly, long-term management of the soybean cyst nematode in modern soybean production will need additional tools, and it is likely that such new tools will be developed from detailed molecular knowledge of the complex Heterodera cyst nematode-plant interactions. This short review provides a snapshot of currently unfolding research discoveries from the genus Heterodera, which also includes other cyst nematodes, particularly the sugar beet cyst nematode H. schachtii, which can infect Arabidopsis and therefore has been used as a model system. Since nematode effectors (the proteins delivered into host plant tissues to mediate parasitism) are at the forefront of nematode–plant interactions, their identification and functional characterization are heavily emphasized in this manuscript

Causes of the growth crisis in the EC countries and strategies for overcoming it

Whereas in 1970 most of the large EC countries were still recording real growth rates in excess of 5%, after the two oil crises there was an appreciable slowdown in economic expansion and at the beginning of the eighties even a real contraction. At the same time unemployment increased dramatically. Where do the causes of the crisis lie? What strategies promise success in overcoming it

Differential Display Analysis of the Early Compatible Interaction Between Soybean and the Soybean Cyst Nematode

The marked cellular changes during feeding site formation of the soybean cyst nematode (Heterodera glycines) indicate major changes in soybean gene expression. We used differential display of mRNA to detect host gene expression changes during the early compatible interaction between soybean and H. glycines. Fifteen cDNA clones corresponding to mRNAs with different abundances in H. glycines-infected versus uninfected roots were identified. Differential display results indicated that abundances of five mRNAs increased in infected roots, whereas abundances of 10 mRNAs decreased. Transcripts for nine of these 15 cDNAs could be detected on RNA blots, and their hybridization signals confirmed the differential display results for eight of these nine cDNAs. Sequence analyses identified five cDNAs with decreased mRNA levels in infected roots as corresponding to two putative aldolase genes, a transcription-factor TFIIA homologue, the soybean small GTP-binding protein gene sra1, and the soybean auxin down-regulated gene ADR12. RNA blot analyses of other auxin down-regulated genes revealed a decrease in their mRNA abundances in H. glycines-infected roots as well

Dynamic Time-Dependent Route Planning in Road Networks with User Preferences

There has been tremendous progress in algorithmic methods for computing driving directions on road networks. Most of that work focuses on time-independent route planning, where it is assumed that the cost on each arc is constant per query. In practice, the current traffic situation significantly influences the travel time on large parts of the road network, and it changes over the day. One can distinguish between traffic congestion that can be predicted using historical traffic data, and congestion due to unpredictable events, e.g., accidents. In this work, we study the \emph{dynamic and time-dependent} route planning problem, which takes both prediction (based on historical data) and live traffic into account. To this end, we propose a practical algorithm that, while robust to user preferences, is able to integrate global changes of the time-dependent metric~(e.g., due to traffic updates or user restrictions) faster than previous approaches, while allowing subsequent queries that enable interactive applications

International Perspectives on Women and Work in Hotels, Catering and Tourism

[Excerpt] Hotels, catering and tourism (HCT) is a large and fast-growing service sector, with an average female participation of 55.5 per cent at global level and up to 70 per cent at regional level. They are employed in a wide variety of roles, including as cleaners and kitchen staff, front-line customer service workers and senior management. The recruitment, retention and promotion of talented women for technical and managerial leadership positions will be necessary to meet the future skills and productivity requirements of the sector. Moreover, women will comprise an even larger proportion of the sector‟s client base as more will travel for business and leisure. This too will have an impact on gender equality in the recruitment of employees. This working paper highlights the structural and cultural issues which determine the roles that women play within the HCT workforce and the strategies which can make a difference to their status and opportunities within the industry. Some of these issues relate to occupational sex segregation, wage parity, career opportunities, the role of women within micro-enterprises and the informal hotel/catering/tourism economy. The links between equality of opportunity and treatment for women and men in quality jobs, workforce development, training opportunities and employment in the sector have been explored to a certain degree at national or local levels. However, there is less information regarding gender equality provisions and major international companies in global and regional contexts. The findings of this study point to issues of importance for employment of women as a basis for future dialogue. They also highlight important innovations, good practices and interesting case studies in support of future human resources planning for governments, employers‟ and workers‟ organizations