Augalinių preparatų vartojimo ypatumai peršalimo ligų prevencijai ir gydymui : (LFS 31-jo suvažiavimo praktinių užsiėmimų santrauka)

ISSN 1648-0066 onlinePeršalimas (ūmi virusinė respiratorinė infekcija) – tai virusinė viršutinių kvėpavimo takų infekcinė liga. Peršalimą sukelia daugiau kaip 200 skirtingų virusų, iš jų dažniausi – rinovirusai, kuriais užsikrečiama kontaktiniu ar oro lašeliniu būdu. Peršalimas pasireiškia visų sezonų metu, dažniausiai atšalus orams, patiriant lėtinį stresą, nusilpus žmogaus imuninei sistemai. Liga dažniausiai trunka 3-7 dienas. Simptomai būna įvairūs, pagrindiniai: skaudanti gerklė ir sloga. Peršalus temperatūra būna neaukšta, arba normali, tačiau ši liga vargina pacientą, mažėja jo darbingumas, blogėja gyvenimo kokybė. Peršalimas gali užsitęsti, tada ligos eigoje atsiranda kosulys.[...]Botanikos sodasLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

Augalų anatomijos ir morfologijos paveikslų rinkinys : metodinė priemonė

Botanikos sodasKauno medicinos universitetasVytauto Didžiojo universiteta

Identification of flavonoids in the flowers and leaves of Monarda didyma L

Monarda didyma L. is a herbaceous perennial with the square stems and opposite leaves characteristic of herbs in the Labiate family. Monarda didyma L. is often used as a domestic medicine, being particularly useful in the treatment of digestive disorders. The leaves and flowering stems are antihelmintic, carminative, diuretic, expectorant, febrifuge, rubefacient and stimulant. Monarda didyma L. has twenty-six compounds in the essential oil, of which thymol, with smaller amounts of para-cymene, d-limonene, carvacrol, linalool, and hydrothymoquinone. A new method was used for the determining of the amount of separate flavonols by high-performance liquid chromatography. The flavonoids (rutin, hyperoside, quercitrin, luteolin, quercetin) were found in leaves and flowers of beebalm. The quantity of flavonoids was higher in flowers than in leaves of Monarda didyma L

Identification of flavonoids in the flowers and leaves of Monarda didyma L

Monarda didyma L. is a herbaceous perennial with the square stems and opposite leaves characteristic of herbs in the Labiate family. Monarda didyma L. is often used as a domestic medicine, being particularly useful in the treatment of digestive disorders. The leaves and flowering stems are antihelmintic, carminative, diuretic, expectorant, febrifuge, rubefacient and stimulant. Monarda didyma L. has twenty-six compounds in the essential oil, of which thymol, with smaller amounts of para-cymene, d-limonene, carvacrol, linalool, and hydrothymoquinone. A new method was used for the determining of the amount of separate flavonols by high-performance liquid chromatography. The flavonoids (rutin, hyperoside, quercitrin, luteolin, quercetin) were found in leaves and flowers of beebalm. The quantity of flavonoids was higher in flowers than in leaves of Monarda didyma L

The dynamics of accumulation of phenolic compounds in the herb of showy tick trefoil (Desmodium Canadense (L.) DC.) along phenological phases of development

Kanadines jakšūnes žolėje nustatyta 15 avonoidų (aglikonų ir glikozidų): viceninas-2, orientinas, homoorientinas, viteksino ramnozidas, viteksinas, izoviteksinas, rutinas, hiperozidas, apigenin-7-O-gliukozidas, astragalinas, kvercitrinas, liuteolinas, kvercetinas, apigeninas ir kemferolis bei dvi fenolkarboksirūgštys: chlorogeno rūgštis ir kavos rūgštis. Biologiškai aktyviu fenoliniu junginių kiekis žolėje tirtas nuosekliais fenologiniais vystymosi tarpsniais: butonizacijos, žydėjimo pradžios, žydėjimo, vaisių brandos pradžios ir visiškos vaisių brandos. Veikliųjų medžiagų kiekis svyravo nuo 12 948,90 μg/g butonizacijos tarpsnyje iki 13 844,05 μg/g žydėjimo pradžios tarpsnyje. Tarp identifikuotų fenolinių junginių dominavo avonoidai: orientinas, homoorientinas, viceninas-2, viteksinas, izoviteksinas ir rutinas. Žydėjimo pradžios tarpsnyje orientinas ir homoorientinas sudarė 45,09 proc. visų veikliujų junginių, izoviteksinas – 12,78 proc., viceninas-2 – 14,75 proc., viteksinas –12,41 proc., rutinas – 6,69 proc. Tai sudaro 91,7 proc. bendro suminio visų identifikuotų veikliųjų fenolinių junginių kiekio. Vaistinę augalinę žaliavą – kanadinės jakšūnės žolę rekomenduojama rinkti žydėjimo pradžioje, kai joje susikaupia daugiausia veikliųjų junginiųIn the herb of Showy tick trefoil 15 avonoids of aglicone and glycoside nature (vicenin-2, orientin, homoorientin, vitexin ramnoside, vitexin, isovitexin, rutin, hiperosid, apigenin-7-O-glucoside, astragalin, quercitrin, luteolin, quercetin, apigenin, cemferol) and 2 phenolcarboxylic acids (chlorogenic acid and ca eic acid) were identi ed. e amount of biologically active phenolic compounds was investigated in the consecutive phenological phases of development: butonization, the beginning of owering, owering, the beginning of the ripeness of fruits and full ripeness of fruits. e amount of active substances uctuated from 12 948,90 μg/g in the butonization phase till 13 844,05 μg/g in the phase of the begining of owering. Among the identi cated phenolic compounds avonoids orientin, homoorientin, vicenin-2, vitexin, isovitexin and rutin predominated: orientin and homoorientin formed 45,09 % of the total amount of all active compounds, isovitexin – 12,78 %, vicenin-2 – 14,75 %, vitexin – 12,41 %, rutin – 6,69 %. ey composed 91,7 % of the total amount of the identi ed active phenolic compounds. us, we recommend to prepare the medicinal raw material - herb of Showy tick trefoil at the phase of the beginning of owering, when the amount of biologically active phenolic compounds in the herb reaches its maximumBotanikos sodasKauno medicinos universitetasVytauto Didžiojo universiteta

Screening of chemical composition of summer savory essential oils using solid-phase microextraction and GC-MC

Problems related to medicinal, spice (aromatic) plants species diversity, enhancing and improving human health are solved according to the Global Strategy for Plant Conservation and the Convention on Biological Diversity adopted regulations [1, 2]. The aim of this study is to investigate chemical composition of medicinal (aromatic) plant Summer savory. The object – Summer savory (Satureja hortensis L.) – annual herbaceous plant from Lamiaceae Lindl. introduced in Lithuania in Kaunas Botanical Garden of Vytautas Magnus University since 1962 [3, 4]. The raw material of the medicinal plant is herb of Savory (Herba Saturejae). Aerial parts of plant are widely used in traditional medicine for cramps, digestion disorders and inflammations. Savory has antimicrobial, antiviral, antispasmodic, anti-inflammatory, antifungal and antidiarrheal properties related to high concentration of essential oils and polyphenols. The main biological active substances of essential oils are carvacrol, thymol, γ-terpinen, thymoquinone, o-cymene and β-caryophyllene. Phytochemical analysis of medicinal plant was performed using solid-phase microextraction and gas chromatography-mass spectrometry by determining essential oil components of Satureja hortensis L. raw material [5, 6]. The results obtained during qualitative and quantitative evaluation of the raw material essential oil demonstrated that this medicinal (aromatic) plant can be successfully cultivated in Lithuania and used in the pharmaceutical and food industriesBiochemijos katedraBotanikos sodasLietuvos sveikatos mokslų universitetasVytauto Didžiojo universiteta

Determination of total phenolics, flavonoids and radical scavenging activity of Summer savory (Satureja hortensis L.) methanolic extracts

The aim was to estimate changes of total phenolics, total flavonoids and radical scavenging activity of Summer savory (Satureja hortensis L.) methanolic extracts during vegetation in middle Lithuania edafo-climatic conditions. The object. Summer savory (Satureja hortensis L.) is the annual herbaceous plant from Labiatae Lindl. family originated from Mediterranean area and Middle East. This medicinal (aromatic) plant has been introduced in Kaunas Botanical Garden of Vytautas Magnus University since 1962 [1]. According to the literature summer savory has significant antioxidant activity related to the high phenolic content [2,3,4]. The raw material – Savory herb (Herba Saturejae) is widely used in traditional medicine for treatment of digestion disorders, cramps, cold and respiratory infections [5]. In this study phytochemical analysis of medicinal plants methanolic extracts was performed using spectrophotometry methods. The Folin-Ciocalteu spectrophotometric method was applied for determination of the total phenolic content. Estimation of total flavonoids was determined using a colorimetric aluminum chloride method. In order to detect antioxidant activity, a method based on the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) was carried out. Results. The amounts of total phenolics, total flavonoids and radical scavenging activity were determined in five vegetation phases (intensive growing, butonisation, beginning of flowering, massive flowering, end of flowering). Spectrophotometric analysis revealed, that total amount of phenolic compounds and flavonoids in Satureja hortensis L. significantly changed during the plant vegetation. The highest concentration of these compounds was determined in butonization phase. The radical scavenging activity was caried out using DPPH radical, the correlation between total phenolic content with radical scavenging activity was observed. Conclusion. Determined phenolic comp...[...]Biochemijos katedraBotanikos sodasLietuvos sveikatos mokslų universitetas. Medicinos akademijaVytauto Didžiojo universiteta

Quantitative HPLC estimation of flavonoids in showy tick trefoil herbs

Quantitative and qualitative analysis of flavonoids in showy tick trefoil [Desmodium canadense (L.) DC., Fabaceae] herbs collected in various vegetative phases at the Department of Medicinal Herbs of the Kaunas Botanical Garden (Vytautas Magnus University) was performed using HPLC techniques. A total of 15 flavonoids were identified as aglycons and glycosides including apigenin, apigenin-7-O-glycoside, luteolin, rutin, vicenin-2, vitexin, isovitexin, vitexin rhamnoside, orientin, homoorientin, quercitrin, quercetin, hyperoside, astragalin, and kaempferol. The maximum amount of identified flavonoids (13573.83 g g) was observed at the budding stage. Among these flavonoids, orientin and homoorientin (6236.24 g g), vicenin-2 (2040.25 g g), vitexin (1717.12 g g), isovitexin (1767.82 g g), and rutin (925.72 g g) predominatedBotanikos sodasHerbaria Drugstore, KaunasKauno medicinos universitetasVytauto Didžiojo universiteta

Quantitative HPLC estimation of flavonoids in showy tick trefoil herbs

Quantitative and qualitative analysis of flavonoids in showy tick trefoil (Desmodium canadense L., Fabaceae family) herbs collected in various phases of vegetation at the Department of Medicinal Herbs of the Kaunas Botanical Garden (Vytautas Magnus University) was performed using HPLC techniques. A total of fifteen flavonoids of the aglicone and glycoside nature were identified, including apigenin, apigenin-7-O-glycoside, luteolin, rutin, vicenin-2, vitexin, isovitexin, vitexin rhamnoside, orientin, homoorientin, quercitrin, quercetin, hyperoside, astragalin, and kaempferol. The maximum amount of identified flavonoids (13573.83 mg/g) was observed at the budding stage. Among these flavonoids, orientin and homoorientin (6236.24 μg/g), vicenin-2 (2040.25 μg/g), vitexin (1717.12 μg/g), isovitexin (1767.82 μg/g) and rutin (925.72 μg/g) predominatedBotanikos sodasKauno medicinos universitetasKauno medicinos universitetas, [email protected] Didžiojo universiteta

Quantitative HPLC estimation of flavonoids in showy tick trefoil herbs

Проведено многолетнее исследование качественного и количественного содержаниия флавоноидов в траве десмодиума канадского (Desmodium canadense (L.) DC., Fabaceae), собранной в различные фазы вегетации в отделе лекарственных растений Каунасского ботанического сада Университета Витаутаса Великого. Методом ВЭЖХ определено 15 флавоноидов агликоновой и гликозидной природы: апигенин, апигенин-7-O-гликозид, лютеолин, рутин, виценин-2, витексин, изовитексин, витексина рамнозид, ориентин, гомоориентин, кверцитрин, кверцетин, гиперозид, астрагалин и кемферол. По многолетним исследованиям наибольшее количество идентифицированных вещест в траве достигалось в фазе цветения растения: 2,45 % на 2-м году вегетации и 1,28 % на 6-м году вегетации, т.е. количество их постепенно уменьшалось в процессе вегетации растения. Среди них oриентин и гомоориентин составлял от 45,9 до 38,9 % от общего количества флавоноидов, виценин-2 — от 16,3 до 12,8 %, витексин — от 12,7 до 8,6 %, изовитексин — от 13,0 до 7,7 %, рутин — от 11,6 до 6,9 %. [...]Botanikos sodasLietuvos sveikatos mokslų universitetasLietuvos sveikatos mokslų universitetas, [email protected] žemdirbystės instituto Vokės filialasLietuvos žemdirbystės instituto Vokės filialas, [email protected] Didžiojo universiteta