Polymerization-Induced Self-Assembly of Block Copolymer Nano-objects via RAFT Aqueous Dispersion Polymerization

In this Perspective, we discuss the recent development of polymerization-induced self-assembly mediated by reversible addition–fragmentation chain transfer (RAFT) aqueous dispersion polymerization. This approach has quickly become a powerful and versatile technique for the synthesis of a wide range of bespoke organic diblock copolymer nano-objects of controllable size, morphology, and surface functionality. Given its potential scalability, such environmentally-friendly formulations are expected to offer many potential applications, such as novel Pickering emulsifiers, efficient microencapsulation vehicles, and sterilizable thermo-responsive hydrogels for the cost-effective long-term storage of mammalian cells

Effects of Anacetrapib in Patients with Atherosclerotic Vascular Disease

BACKGROUND: Patients with atherosclerotic vascular disease remain at high risk for cardiovascular events despite effective statin-based treatment of low-density lipoprotein (LDL) cholesterol levels. The inhibition of cholesteryl ester transfer protein (CETP) by anacetrapib reduces LDL cholesterol levels and increases high-density lipoprotein (HDL) cholesterol levels. However, trials of other CETP inhibitors have shown neutral or adverse effects on cardiovascular outcomes. METHODS: We conducted a randomized, double-blind, placebo-controlled trial involving 30,449 adults with atherosclerotic vascular disease who were receiving intensive atorvastatin therapy and who had a mean LDL cholesterol level of 61 mg per deciliter (1.58 mmol per liter), a mean non-HDL cholesterol level of 92 mg per deciliter (2.38 mmol per liter), and a mean HDL cholesterol level of 40 mg per deciliter (1.03 mmol per liter). The patients were assigned to receive either 100 mg of anacetrapib once daily (15,225 patients) or matching placebo (15,224 patients). The primary outcome was the first major coronary event, a composite of coronary death, myocardial infarction, or coronary revascularization. RESULTS: During the median follow-up period of 4.1 years, the primary outcome occurred in significantly fewer patients in the anacetrapib group than in the placebo group (1640 of 15,225 patients [10.8%] vs. 1803 of 15,224 patients [11.8%]; rate ratio, 0.91; 95% confidence interval, 0.85 to 0.97; P=0.004). The relative difference in risk was similar across multiple prespecified subgroups. At the trial midpoint, the mean level of HDL cholesterol was higher by 43 mg per deciliter (1.12 mmol per liter) in the anacetrapib group than in the placebo group (a relative difference of 104%), and the mean level of non-HDL cholesterol was lower by 17 mg per deciliter (0.44 mmol per liter), a relative difference of -18%. There were no significant between-group differences in the risk of death, cancer, or other serious adverse events. CONCLUSIONS: Among patients with atherosclerotic vascular disease who were receiving intensive statin therapy, the use of anacetrapib resulted in a lower incidence of major coronary events than the use of placebo. (Funded by Merck and others; Current Controlled Trials number, ISRCTN48678192 ; ClinicalTrials.gov number, NCT01252953 ; and EudraCT number, 2010-023467-18 .)

Can grasslands enhance the ecological service of pollinators? Effects of number of cuttings on availability of floral resources for flower-visiting insects

Bees and other flower-visiting insects are experiencing periods of food shortage, particularly in intensively managed agricultural landscapes in Europe. As part of the Danish project EcoServe (http://agro.au.dk/ecoserve/ ) we therefore investigated whether increased richness of selected herbaceous species in organic hay fields acted as a floral resource thus enhancing abundance and biodiversity of pollinators. Moreover, we investigated if availability of floral resources was affected by cutting regime (timing and number of cuttings during the growing season) in 11 herbaceous species, which are often used in grassland seed mixtures. In a field survey, we visited 20 organic hay meadows of different age (3-100 years), and conducted surveys regularly throughout the flowering season to quantify diversity of insect-pollinated plants and pollinators. Our results showed that diversity of pollinators was significantly and positively related to diversity of plants, but that hay fields embedded in a complex landscape harbored a higher diversity of both plants and insects than hay fields in more homogeneous areas. Also, diversity of plants and insects increased in older hay meadows. In an experimental set-up, we tested the availability of flowers in plots of monocultures and a mixture of 11 herbaceous species under two cutting regimes: (1) plots were cut once every four weeks throughout the growing season, and (2) plots were cut only at the end of the flowering season. While the majority of species provided flowers for pollinators when plots were not cut during the flowering season, most plant species set few if any flowers if cut frequently. One exception was Trifolium repens, which was slightly stimulated by cutting. Thus, traditional grasslands which are frequently mowed are expected to be poor in flowers. Our results suggest that it is important to leave unmowed flower-rich areas in the fields throughout the flowering season to avoid periods of food shortage. The potential for unmowed hay fields to enhance diversity and abundance of pollinators, however, depend on the composition of the surrounding landscape and age of the field

Discrimination of Arcobacter butzleri isolates by polymerase chain reaction-mediated DNA fingerprinting

Aims: The objective of this study was to subtype Arcobacter butzleri isolates using RAPD-PCR.AIMS: The objective of this study was to subtype Arcobacter butzleri isolates using RAPD-PCR.METHODS AND RESULTS: Thirty-five A. butzleri isolates obtained from chicken carcasses were examined. PCR-mediated DNA fingerprinting technique with primers of the variable sequence motifs was used to detect polymorphism within the isolates. Eleven distinct DNA profiles were obtained as follows: Of the 35 strains, 10 as profile 4; seven as profile 1; five as profile 3; three as profiles 2 and 9; two as profile 10; one as profiles 5, 6, 7, 8 and 11.CONCLUSIONS: Chicken carcasses sold in markets were found to be contaminated with several different strains of A. butzleri. RAPD-PCR technique was found to be a useful technique for distinguishing A. butzleri isolates.SIGNIFICANCE AND IMPACT OF THE STUDY: The presence of several different A. butzleri strains on chicken carcasses may indicate multiple sources of contamination. The epidemiological role of A. butzleri in human and animal diseases should be investigated further