3 research outputs found
Simultaneous Determination Of Ezetimibe And Atorvastatin Calcium In Binary Mixture By Second Derivative (D 2 ) Spectrofluorimetric Method
A method for the simultaneous determination of ezetimibe (EZB) and atorvastatin calcium (ATVC) was developed, based on the measurement of their native fluorescence signals, by using second-derivative spectrofluorimetry to resolve the mixture. EZB was measured at λ 455.0 nm, and ATVC was measured at λ em = 394.0 nm. Instrumental parameters were optimized, and the emission spectra were recorded between 380.0 and 470.0 nm, at λ em = ex = 246.0 nm and excitation and emission slit widths of 10.0 nm. The calibration graphs were linear over the ranges of 200 – 900 ng/mL for both EZB and ATVC, with a mean percentage recovery of 99.64 ± 0.88 and 99.88 ± 0.61, respectively. Methods were validated according to ICH guidelines and successfully applied for analysis of bulk powder and pharmaceutical formulations. The results were statistically compared to a reported method and no significant difference was noticed regarding accuracy and precision.Australian Journal of Basic and Applied Science
Two new Spectrophotometric Approaches to the Simultaneous Determination of Ezetimibe and Atorvastatin Calcium
Two rapid, simple and sensitive spectrophotometric methods for the quantitative analysis of ezetimibe (EZB) and
atorvastatin calcium (ATVC) in bulk powder and combined tablet form have been developed and validated. The first
method is bivariate calibration and the second method is ratio subtraction in conjunction with second derivative (D
)spectrophotometry. These methods are tested by analyzing synthetic binary mixtures of the above drugs and they are
applied to commercial pharmaceutical preparation of the subjected drugs. Standard deviation is < 2 in the assay of
raw materials and tablets. Methods are validated as per ICH guidelines and statistical comparison of the suggested
methods with the reported spectrophotometric one using F and t tests showed no significant difference regarding
both accuracy and precision.International Journal of Chemical Studie