7 research outputs found
Experience of acetylcholinesterase histochemistry application in the diagnosis of chronic constipation in children
The aim of this study was to review our experience in applying acetylcholinesterase histochemistry for diagnosing colonic dysganglionoses in children. Patients and methods. We analyzed acetylcholinesterase histochemistry results of rectal biopsy specimens obtained from 85 children. The indications for biopsy were suspicion of Hirschsprungās disease in neonates and infants (Group 1; n=21) and older children (Group 2; n=17); megarectum (Group 3; n=44); and colostomy (Group 4; n=3). Specimens were taken at 5 and 10 cm using endoscopic forceps or excised with scissors at 2.5 cm above the dentate line. Acetylcholinesterase activity was evaluated using Karnovsky-Roots method. Results. The diagnosis of Hirschsprungās disease was confirmed in 17 children of the first group and in 3 of the second group. In the third group, 2 children were diagnosed with ultrashort-segment Hirschsprungās disease and 3 children with intestinal neuronal dysplasia. In one case, acetylcholinesterase reaction was false positive. Hirschsprungās disease was diagnosed in 2 children with colostomies; in one case acetylcholinesterase activity caused false-positive results. Colonic dysganglionoses were diagnosed in 78% of infants and in 14% of children over 1 year of age. The diagnostic specificity of acetylcholinesterase in Hirschsprungās disease was 92%. Conclusions. 1) The analysis of acetylcholinesterase activity in childrenās rectal biopsy specimens is a reliable method for diagnosing Hirschsprungās disease, especially in infants; 2) This method of examination is irreplaceable in diagnosing ultrashort-segment Hirschsprungās disease and remains the only method to confirm the diagnosis of this disease; 3) Acetylcholinesterase histochemistry is not sufficiently informative in diagnosing intestinal neuronal dysplasia type B, because authors applying other neurohistochemical investigation methods have reported higher incidence of this disease
AcetilcholinesterazÄs histocheminio tyrimo metodo naudojimo patirtis diagnozuojant vaikų lÄtinÄÆ vidurių užkietÄjimÄ
The aim of this study was to review our experience in applying acetylcholinesterase histochemistry for diagnosing colonic dysganglionoses in children. Patients and methods. We analyzed acetylcholinesterase histochemistry results of rectal biopsy specimens obtained from 85 children. The indications for biopsy were suspicion of Hirschsprungās disease in neonates and infants (Group 1; n=21) and older children (Group 2; n=17); megarectum (Group 3; n=44); and colostomy (Group 4; n=3). Specimens were taken at 5 and 10 cm using endoscopic forceps or excised with scissors at 2.5 cm above the dentate line. Acetylcholinesterase activity was evaluated using Karnovsky-Roots method. Results. The diagnosis of Hirschsprungās disease was confirmed in 17 children of the first group and in 3 of the second group. In the third group, 2 children were diagnosed with ultrashort-segment Hirschsprungās disease and 3 children with intestinal neuronal dysplasia. In one case, acetylcholinesterase reaction was false positive. Hirschsprungās disease was diagnosed in 2 children with colostomies; in one case acetylcholinesterase activity caused false-positive results. Colonic dysganglionoses were diagnosed in 78% of infants and in 14% of children over 1 year of age. The diagnostic specificity of acetylcholinesterase in Hirschsprungās disease was 92%. Conclusions. 1) The analysis of acetylcholinesterase activity in childrenās rectal biopsy specimens is a reliable method for diagnosing Hirschsprungās disease, especially in infants; 2) This method of examination is irreplaceable in diagnosing ultrashort-segment Hirschsprungās disease and remains the only method to confirm the diagnosis of this disease; 3) Acetylcholinesterase histochemistry is not sufficiently informative in diagnosing intestinal neuronal dysplasia type B, because authors applying other neurohistochemical investigation methods have reported higher incidence of this disease
Application of Acetylcholinesterase Histochemistry for the Diagnosis of Hirschsprungās Disease in Neonates and Infants: a Twenty-year Experience
Background. The acetylcholinesterase (AChE) histochemical staining of intestinal mucosal-submucosal biopsy specimens is believed to be the most reliable diagnostic method for Hirschsprungās disease (HD). The aim of our study was to evaluate advantages and disadvantages of this method for HD diagnosis in infants and neonates. Material and Methods. The results of AChE histochemistry of rectal biopsy specimens, obtained from 11 neonates and 29 infants treated in the Clinic of Pediatric Surgery, Hospital of the Lithuanian University of Health Sciences, from 1991 to 2010 were analyzed. AChE activity of neural structures was evaluated using KarnovskyāRoots method modified by El-Badawi and Schenk. Results. Two neonates were diagnosed with HD. The diagnosis was not confirmed in 9 cases, but clinical symptoms progressed in 3 cases, and HD was diagnosed after the repeated biopsy performed in infancy. The results of primary biopsy were rated as false negative. Test sensitivity and specificity in neonates were 40.0% and 100%, respectively. A total of 21 infants were diagnosed with HD. All of them underwent surgery. The diagnosis of HD was confirmed in 20 cases; in one case, intestinal neuronal dysplasia type B was diagnosed. The diagnosis was not confirmed in 8 cases. In infants, the test had a sensitivity of 100% and a specificity of 88.8%. Conclusions. The analysis of AChE activity in r ectal biopsy specimens is a reliable method for diagnosing HD in infancy. This test is less valuable in neonates. If test results are negative, infants should be observed, and if symptoms persist, the biopsy should be repeated at the age of 3 months. Rectal biopsy specimens in neonates should include mucosa and submucosa
AcetilcholinesterazÄs histocheminio tyrimo metodo pritaikymo 20-ties metų patirtis diagnozuojant naujagimių ir kÅ«dikių HirÅ”prungo ligÄ
Background. The acetylcholinesterase (AChE) histochemical staining of intestinal mucosal-submucosal biopsy specimens is believed to be the most reliable diagnostic method for Hirschsprungās disease (HD). The aim of our study was to evaluate advantages and disadvantages of this method for HD diagnosis in infants and neonates. Material and Methods. The results of AChE histochemistry of rectal biopsy specimens, obtained from 11 neonates and 29 infants treated in the Clinic of Pediatric Surgery, Hospital of the Lithuanian University of Health Sciences, from 1991 to 2010 were analyzed. AChE activity of neural structures was evaluated using KarnovskyāRoots method modified by El-Badawi and Schenk. Results. Two neonates were diagnosed with HD. The diagnosis was not confirmed in 9 cases, but clinical symptoms progressed in 3 cases, and HD was diagnosed after the repeated biopsy performed in infancy. The results of primary biopsy were rated as false negative. Test sensitivity and specificity in neonates were 40.0% and 100%, respectively. A total of 21 infants were diagnosed with HD. All of them underwent surgery. The diagnosis of HD was confirmed in 20 cases; in one case, intestinal neuronal dysplasia type B was diagnosed. The diagnosis was not confirmed in 8 cases. In infants, the test had a sensitivity of 100% and a specificity of 88.8%. Conclusions. The analysis of AChE activity in r ectal biopsy specimens is a reliable method for diagnosing HD in infancy. This test is less valuable in neonates. If test results are negative, infants should be observed, and if symptoms persist, the biopsy should be repeated at the age of 3 months. Rectal biopsy specimens in neonates should include mucosa and submucosa
Seksualinio smurto moteriŔkosios lyties aukos tyrimo metodika. I dalis = Sexual violence testing methodology of female victims. Part I
Seksualinio smurto moteriÅ”kosios lyties tyrimo metodikoje apžvelgiami pagrindiniai bendravimo su seksualinio smurto auka principai, dokumentacijos pildymas, sužalojimų ÄÆvertinimas, aptariamas lytiÅ”kai plintanÄių infekcijų tyrimo algoritmas, profilaktinis jų gydymas, pateikiama informacija gydytojui ir aukai dÄl tolesnio tyrimų bei stebÄsenos plano, seksualinio smurto aukos tyrimo protokolasSexual violence testing methodology of female victims analyzes the basic communication principles with a sexual violence victim, documentation completion, injury assessment. Sexually transmitted infections testing algorithm and preventive treatment, information to the doctor and the victim on the further research and follow-up plan is discussed, sexual violence testing protocol is proposedLietuvos Respublikos generalinÄ prokuratÅ«raLietuvos sveikatos mokslų universitetas. Medicinos akademijaParamos vaikams centrasTeisÄs fakultetasUgdymo ir konsultavimo centrasValstybinÄ teismo medicinos tarnybaVieÅ”oji ÄÆstaiga RespublikinÄ Å iaulių ligoninÄVieÅ”osios teisÄs katedraVilniaus universitetasVytauto Didžiojo universiteta
Seksualinio smurto moteriŔkosios lyties aukos tyrimo metodika. II dalis = Sexual violence testing methodology of female victims. Part II
Seksualinio smurto moteriÅ”kosios lyties tyrimo metodikoje apžvelgiami pagrindiniai bendravimo su seksualinio smurto auka principai, dokumentacijos pildymas, sužalojimų ÄÆvertinimas, aptariamas lytiÅ”kai plintanÄių infekcijų tyrimo algoritmas, profilaktinis jų gydymas, pateikiama informacija gydytojui ir aukai dÄl tolesnio tyrimų bei stebÄsenos plano, seksualinio smurto aukos tyrimo protokolasSexual violence testing methodology of female victims analyzes the basic communication principles with a sexual violence victim, documentation completion, injury assessment. Sexually transmitted infections testing algorithm and preventive treatment, information to the doctor and the victim on the further research and follow-up plan is discussed, sexual violence testing protocol is proposedLietuvos Respublikos generalinÄ prokuratÅ«raLietuvos sveikatos mokslų universitetas. Medicinos akademijaParamos vaikams centrasTeisÄs fakultetasUgdymo ir konsultavimo centrasValstybinÄ teismo medicinos tarnybaVieÅ”oji ÄÆstaiga RespublikinÄ Å iaulių ligoninÄVieÅ”osios teisÄs katedraVilniaus universitetasVytauto Didžiojo universiteta