Parasitos em assembleia de peixes de diferentes níveis tróficos na bacia igarapé Fortaleza, estado do Amapá, Brasil.

Enbrapoa

Albendazole is effective in controlling monogeneans in Colossoma macropomum (Serrasalmidae): therapeutic baths and their physiological and histopathological effects.

Na aquicultura mundial, a maioria dos quimioterápicos utilizados no controle e tratamento de doenças, são produtos químicos não regulamentados e oriundos da agricultura. Neste estudo, foi investigada a eficácia de banhos terapêuticos com albendazol contra monogeneas Anacanthorus spathulatus, Notozothecium janauachensis e Mymarothecium boegeri das brânquias de Colossoma macropomum, e os efeitos hematológicos e histopatológicos causados por esse anti-helmíntico nos peixes. Foram usados 500 mg/L de albendazol e um grupo controle (água do tanque de cultivo) com três banhos de 24 horas, mantendo intervalos de 24 horas entre cada banho, usando três repetições cada. Ao final dos banhos terapêuticos, foram realizadas análises hematológicas, histopatológicas e parasitológicas. Verificou-se que banhos terapêuticos com 500 mg/L de albendazol apresentaram alta eficácia (94,9%) contra monogenéticos de C. macropomum, causando poucas alterações fisiológicas e histopatológicas. Portanto, banhos com 500 mg/L de albendazol, na estratégia usada, podem ser recomendados para controle e tratamento de infecções por monogenéticos em C. macropomum

Parasite fauna of tambaqui reared in net-cages at two stocking densities.

This study investigated the parasite fauna in Colossoma macropomum reared at two stocking densities in net-cages of a fish farm in the Matapi River, State of Amapá. Before stocking fish in the net-cages for fattening, fish were examined for parasites and also at the end of 90 and 180 days at densities of 50 and 100 fish m-3, respectively. All fish were parasitized by one or more species such as Ichthyophthirius multifiliis, Piscinoodinium pillulare, Anacanthorus spathulatus, Mymarothecium boegeri, Notozothecium janauachensis, Procamallanus (Spirocamallanus) inopinatus and Acarina gen. sp., but the dominance was of I. multifiliis. Parasites presented aggregated dispersion pattern, and there were no differences in the fish relative condition factor between the densities used. There was positive correlation of I. multifiliis abundance and monogenean abundance with length and weight of the hosts. Before fish stocking, the mean intensity and mean abundance of I. multifiliis, A. spathulatus and M. boegeri were lowest when comparing fish at densities of 50 and 100 fish m-3, which were similar to each other. The mean intensity and mean abundance of N. janauachensis was highest in the density of 50 fish m-3 when comparing the fish before stocking and at a density of 100 fish m-³. The mean abundance of P. (S.) inopinatus was highest in fish kept at 100 fish m-³ when compared to fish before stocking and 50 fish m-³. However, P. pillulare and mites occurred only in density of 50 fish m-3. There was a predominance of ectoparasites and few endoparasites, as expected. Therefore, it is necessary the constant monitoring of the parasites, to better implement control strategies aiming to avoid the occurrence of diseases.Scientific Note

Fish Oil Supplementation Reduces Inflammation but Does Not Restore Renal Function and Klotho Expression in an Adenine-Induced CKD Model

BACKGROUND: Chronic kidney disease and inflammation promote loss of Klotho expression. Given the well-established anti-inflammatory effects of omega-3 fatty acids, we aimed to investigate the effect of fish oil supplementation in a model of CKD. METHODS: Male C57BL/6 mice received supplementation with an adenine-enriched diet (AD, n = 5) or standard diet (CTL, n = 5) for 10 days. Two other experimental groups were kept under the adenine diet for 10 days. Following adenine withdrawal on the 11th day, the animals returned to a standard diet supplemented with fish oil (Post AD-Fish oil, n = 9) or not (Post AD-CTL, n = 9) for an additional period of 7 days. RESULTS: Adenine mice exhibited significantly higher mean serum urea, creatinine, and renal expression of the pro-inflammatory markers Interleukin-6 (IL-6), C-X-C motif chemokine 10 (CXCL10), and Interleukin-1β (IL-1β), in addition to prominent renal fibrosis and reduced renal Klotho gene expression compared to the control. Post AD-Fish oil animals demonstrated a significant reduction of IL-6, C-X-C motif chemokine 9 (CXCL9), and IL-1β compared to Post AD-CTL animals. However, serum creatinine, renal fibrosis, and Klotho were not significantly different in the fish oil-treated group. Furthermore, renal histomorphological changes such as tubular dilatation and interstitial infiltration persisted despite treatment. CONCLUSIONS: Fish oil supplementation reduced renal pro-inflammatory markers but was not able to restore renal function nor Klotho expression in an adenine-induced CKD model

Diffuse large B-cell lymphoma of Waldeyer's ring has distinct clinicopathologic features: a GELA study

Background Diffuse large B-cell lymphomas (DLBCLs) arising in specific extranodal sites have peculiar clinicopathologic features. Patients and methods We analyzed a cohort of 187 primary Waldeyer's ring (WR) DLBCLs retrieved from GELA protocols using anthracyclin-based polychemotherapy. Results Most patients (92%) had stage I-II disease. A germinal center B-cell-like (GCB) immunophenotype was observed in 61%, and BCL2 expression in 55%, of WR DLBCLs. BCL2, BCL6, IRF4 and MYC breakpoints were observed in, respectively, 3 of 42 (7%), 9 of 36 (25%), 2 of 26 (8%) and 4 of 40 (10%) contributive cases. A variable follicular pattern was evidenced in 30 of 68 (44%) large biopsy specimens. The 5-year progression-free survival (PFS) and the overall survival (OS) of 153 WR DLBCL patients with survival information were 69.5% and 77.8%, respectively. The GCB immunophenotype correlated with a better OS (P=0.0015), while BCL2 expression predicted a worse OS (P=0.037), an effect overcome by the GCB/non-GCB classification. Compared with matched nodal DLBCLs, WR DLBCLs with no age-adjusted international prognostic index factor disclosed a better 5-year PFS rate (77.5% versus 70.7%; P=0.03). Conclusions WR DLBCLs display distinct clinicopathologic features compared with conventional DLBCLs, with usual localized-stage disease, common follicular features and a high frequency of GCB immunophenotype contrasting with a low rate of BCL2 rearrangements. In addition, they seem to be associated with a better outcome than their nodal counterpar

Eficácia do óleo de Carapa guianensis (Meliaceae) contra infestações de monogenéticos em Colossoma macropomum e seus efeitos hematológicos e histopatológicos.

Este estudo teve como objetivo investigar a eficácia de banhos terapêuticos com óleo de Carapa guianensis (andiroba) contra monogenéticos de Colossoma macropomum (tambaqui), bem como os efeitos hematológicos e histológicos nos peixes expostos.Enbrapoa

Carbon emissions in hydromorphic soils from an estuarine floodplain forest in the Amazon River.

Carbon dioxide (CO2) is produced only in biological activities. Understanding how soil tillage practices affect the dynamics of CO2 production is important, as these processes are influenced by the temperature and humidity conditions of the place. This paper aimed at quantifying CO2 flux in hydromorphic floodplain soils under different açai palm tree grove management strategies, correlating it with litter deposition, soil environment, and season of the year. Conducted in the city of Mazagão-AP, four areas of açai palm tree groves were selected with different types of management. During the evaluation period (October, November, and December 2012, and February, March, and April 2013), CO2 flux, soil moisture, and temperature were measured, and litter samples were collected. In addition, rainfall data for the region were also obtained. The CO2 fluxes obtained ranged from 0.37 to 28.55 μmol CO2 m-2 s-1, with a total average of 6.20 μmol CO2 m-2 s-1. In broad analysis, soil variables did not show significant correlations with CO2 emissions. A positive relationship between flux and litter and soil temperature, as well as a negative relationship with its moisture, were observed only in a few months and specific systems. A produção de dióxido de carbono (CO2) do solo de várzea está relacionada às atividades biológicas, interagindo com sua dinâmica de inundação e manejo. Compreender a forma pela qual práticas de manejo de açaizais afetam as dinâmicas da produção de CO2 é importante, pois elas podem aumentar a emissão em relação à floresta. O objetivo do trabalho foi quantificar o fluxo de CO2 do solo hidromórfico de várzea sob diferentes manejos de açaizais, analisando suas relações com a deposição de serapilheira, ambiente do solo e o período do ano. Realizado no município de Mazagão-AP, foram selecionadas quatros áreas de açaizais com diferentes tipos de manejos. Durante o período avaliado (out/2012, nov/2012, dez/2012, fev/2013, mar/2013 e abr/2013), abrangendo períodos sem inundação (verão amazônico) e com inundação (inverno), foram medidos o fluxo de CO2, umidade e temperatura do solo, e deposição de serapilheira. Além disso, também foram obtidos dados de precipitação da região. O fluxo de CO2 variou de 0,37 a 28,55 μmol CO2 m-2 s-1, com média de 6,20 μmol CO2 m-2 s-1. No geral, as variáveis do solo não apresentaram correlações significativas com a emissão de CO2. Apenas em alguns meses e em sistemas específicos, observou-se relação positiva do fluxo com a serapilheira e temperatura do solo e relação negativa com sua umidade

Essential oil of Piper hispidum (Piperaceae) has efficacy against monogeneans, and effects on hematology and gill histology of Colossoma macropomum.

Abstract: This study investigated for the first time the effectiveness of therapeutic baths with essential oil (EO) of Piper hispidum against monogeneans Anacanthorus spathulatus, Notozothecium janauachensis, Mymarothecium boegeri and Linguadactyloides brinkmanni from the gills of Colossoma macropomum, as well as the hematological and histological effects on this fish. Resumo: Este estudo investigou pela primeira vez, a eficácia de banhos terapêuticos com óleo essencial (OE) de Piper hispidum contra monogenéticos Anacanthorus spathulatus, Notozothecium janauachensis, Mymarothecium boegeri e Linguadactyloides brinkmanni das brânquias de Colossoma macropomum, bem como os efeitos hematológicos e histológicos neste peixe.Título em Português: Óleo de Piper hispidum (Piperaceae) tem eficácia contra monogenéticos, e efeitos na hematologia e histologia de brânquias de Colossoma macropomum. Onde se lê Francisco Célio Maia Chagas, leia-se Francisco Célio Maia Chaves

Characterization of Generalized Young Measures Generated by Symmetric Gradients

This work establishes a characterization theorem for (generalized) Young measures generated by symmetric derivatives of functions of bounded deformation (BD) in the spirit of the classical Kinderlehrer\ue2\u80\u93Pedregal theorem. Our result places such Young measures in duality with symmetric-quasiconvex functions with linear growth. The \ue2\u80\u9clocal\ue2\u80\u9d proof strategy combines blow-up arguments with the singular structure theorem in BD (the analogue of Alberti\ue2\u80\u99s rank-one theorem in BV), which was recently proved by the authors. As an application of our characterization theorem we show how an atomic part in a BD-Young measure can be split off in generating sequences