Türkiyenin Güney Ege Bölgesinde alg verimliliği çalışmaları

Bu çalışmada Güney Ege Denizi'ndeki 11 farklı bölgeden periyodik toplanılan Ulva lactuca Linnaeus, Cystoseira compressa (Esper) Gerloff et Nizamuddin, C. crinita Duby, C. elegans Sauvageau, C. schiffneri G.Hamel, Hypnea musciformis (Wulfen) J.V.Lamaouroux, Laurencia papillosa (C.Agardh) Greville and Halopithys incurva (Hudson) Batters türlerinin kuru ağırlıkları karşılaştınlmıştır. Ayrıca, ekonomik olan bu türlerin uygun toplanma zamanlan önerilmiştir.In this study, Ulva lactuca Linnaeus, Cystoseira compressa (Esper) Gerloff et Nizamuddin, C. crinita Duby, C. elegans Sauvageau, C. schiffneri G.Hamel, Hypnea musciformis (Wulfen) J.V.Lamaouroux, Laurencia papillosa (C.Agardh) Greville and Halopithys incurva (Hudson) Batters were collected from 11 stations of South Aegean Sea. the dry and fresh weights of these algae were compared. in addition, collection periods of these commercial species were suggested

Purification of phycocyanin from isolated and identified hot spring cyanobacteria

338-345Phycocyanin is a phycobiliprotein used as a healthy food additive as well as an ingredient in cosmetic dye preparation. In this study, we attempted molecular and morphological identification of three thermophilic filamentous cyanobacteria and also explored a method to separate and purify thermostable C-phycocyanin of analytical grade from them for biotechnological applications. Cyanobacteria strains were collected from hot springs in Karakoc-Seferihisar, Gulbahce-Urla and Sifne-Cesme in Izmir, Turkey. The samples were identified both by morphological observations and genetic assay. Filamentous cyanobacteria DNA from the collected samples was isolated and extracted, and were analyzed using a 16S rRNA cyanobacteria-specific PCR and a denaturing-gradient gel electrophoresis (DGGE). Extraction and purification of phycocyanin was performed in two stages, ammonium sulfate saturation and dialysis, gel filtration and ion exchange chromatography. Conventional separation of Geitlerinema sp., Halospirulina sp. and Phormidium animale was successfully performed yielding C-phycocyanin at 493.760±3.610, 89.060±3.209, 32.978±0.350, 4.046±0.193, 8.303±0.511 and 4.196±0.090 mg/g purity from gel filtration and ion exchange chromatography processes, respectively. The SDS-PAGE demonstrated that the P. animale, Halospirulina sp. and Geitlerinema sp. were purified phycocyanin. Overall, in this study, three cyanobacteria were isolated and cultivated in a laboratory conditions, and then the cyanobacterial cells was extracted and purified thermostable C-phycocyanin obtained, which may be used as raw material in food supplement and pharmaceutical industry

Purification of phycocyanin from isolated and identified hot spring cyanobacteria

###EgeUn###Phycocyanin is a phycobiliprotein used as a healthy food additive as well as an ingredient in cosmetic dye preparation. In this study, we attempted molecular and morphological identification of three thermophilic filamentous cyanobacteria and also explored a method to separate and purify thermostable C-phycocyanin of analytical grade from them for biotechnological applications. Cyanobacteria strains were collected from hot springs in Karakoc-Seferihisar, Gulbahce-Urla and Sifne-Cesme in Izmir, Turkey. The samples were identified both by morphological observations and genetic assay. Filamentous cyanobacteria DNA from the collected samples was isolated and extracted, and were analyzed using,a 16S rRNA cyanobacteria-specific PCR and a denaturing-gradient gel electrophoresis (DGGE). Extraction and purification of phycocyanin was performed in two stages, ammonium sulfate saturation and dialysis, gel filtration and ion exchange chromatography. Conventional separation of Geitlerinema sp., Halospirulina sp. and Phormidium animale was successfully performed yielding C-phycocyanin at 493.760 +/- 3.610, 89.060 +/- 3.209, 32.978 +/- 0.350, 4.046 +/- 0.193, 8.303 +/- 0.511 and 4.196 +/- 0.090 mg/g purity from gel filtration and ion exchange chromatography processes, respectively. The SDS-PAGE demonstrated that the P. animale, Halospirulina sp. and Geitlerinema sp. were purified phycocyanin. Overall, in this study, three cyanobacteria were isolated and cultivated in a laboratory conditions, and then the cyanobacterial cells was extracted and purified thermostable C-phycocyanin obtained, which may be used as raw material in food supplement and pharmaceutical industry.Scientific and Technological Research Council of Turkey-TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109T037]; Ege University Science and Technology Centre-EBILTEMEge University [09 BIL 027]This study was financially supported by The Scientific and Technological Research Council of Turkey-TUBITAK (109T037) project and Ege University Science and Technology Centre-EBILTEM (09 BIL 027) project