Source apportionment and Elemental Composition of Atmospheric total Suspended Particulates (TSP) Over the Red Sea Coast of Saudi Arabia

This work presents a comprehensive study on concentrations and elemental composition of total suspended atmospheric particulates for a semi-urban site on the Red Sea coast, and on-board a research vessel, which collected off-shore samples along the Red Sea. We conducted one of the most extended measurement campaigns of atmospheric particulates ever for the region, with continuous measurements over 27 months. The overall mean concentrations (± st. dev.) of TSP were 125 ± 197 µg m−3 for the permanent semi-urban site, and 108 ± 193 µg m−3 for the off-shore mobile site. The region is frequently severely impacted by both localised and widespread dust storms, which on occasion, can increase atmospheric particulate concentrations to levels above mg m−3 (> 1000 µg m−3). Median concentrations were not as variable between seasons, indicating a stable, permanent presence of atmospheric particulates independent of the time of year. The primary chemical elements contributing to particulate mass were Na, Ca, S, Al and Fe. We employed Positive Matrix Factorisation (EPA PMF v5.0.14) to identify different major sources of particulates, which were crustal, marine, fuel oil combustion/secondary sulphate and mixed anthropogenic. The crustal source was characterised by tracers Al, Fe, K, Mg and Sn, and was present to some extent in the other identified sources due to the permanent presence of dust particles in the atmosphere. The fuel oil combustion/secondary sulphate source was identifiable by the almost exclusive presence of S, and to a lesser extent V, emitted from oil combustion as primary emissions and also secondary sulphate formation following the release of S to the atmosphere. A mixed anthropogenic source was characterised by Zn, Ni, Cr, Cu and Pb, emitted from traffic, industry, power generation and water desalination. This study highlights that the natural sources of particulates in this desert region give rise to frequent episodes of extremely poor air quality, and this problem is compounded by significant emissions of anthropogenic pollution, which has an impact across the entire Red Sea basin. Further stringent measures should be adopted to improve air quality across the region and prevent long-term damage to the health of the local population and ecosystems.En prens

Airborne Prokaryote and Virus Abundance Over the Red Sea

Aeolian dust exerts a considerable influence on atmospheric and oceanic conditions negatively impacting human health, particularly in arid and semi-arid regions like Saudi Arabia. Aeolian dust is often characterized by its mineral and chemical composition; however, there is a microbiological component of natural aerosols that has received comparatively little attention. Moreover, the amount of materials suspended in the atmosphere is highly variable from day to day. Thus, understanding the variability of atmospheric dust loads and suspended microbes throughout the year is essential to clarify the possible effects of dust on the Red Sea ecosystem. Here, we present the first estimates of dust and microbial loads at a coastal site on the Red Sea over a 2-year period, supplemented with measurements from dust samples collected along the Red Sea basin in offshore waters. Weekly average dust loads from a coastal site on the Red Sea ranged from 4.6 to 646.11 μg m−3, while the abundance of airborne prokaryotic cells and viral-like particles (VLPs) ranged from 77,967 to 1,203,792 cells m−3 and from 69,615 to 3,104,758 particles m−3, respectively. To the best of our knowledge, these are the first estimates of airborne microbial abundance in this region. The elevated concentrations of resuspended dust particles and suspended microbes found in the air indicate that airborne microbes may potentially have a large impact on human health and on the Red Sea ecosystem.En prens

Enhanced Viral Activity in the Surface Microlayer of the Arctic and Antarctic Oceans

The ocean surface microlayer (SML), with physicochemical characteristics different from those of subsurface waters (SSW), results in dense and active viral and microbial communities that may favor virus–host interactions. Conversely, wind speed and/or UV radiation could adversely affect virus infection. Furthermore, in polar regions, organic and inorganic nutrient inputs from melting ice may increase microbial activity in the SML. Since the role of viruses in the microbial food web of the SML is poorly understood in polar oceans, we aimed to study the impact of viruses on prokaryotic communities in the SML and in the SSW in Arctic and Antarctic waters. We hypothesized that a higher viral activity in the SML than in the SSW in both polar systems would be observed. We measured viral and prokaryote abundances, virus-mediated mortality on prokaryotes, heterotrophic and phototrophic nanoflagellate abundance, and environmental factors. In both polar zones, we found small differences in environmental factors between the SML and the SSW. In contrast, despite the adverse effect of wind, viral and prokaryote abundances and virus-mediated mortality on prokaryotes were higher in the SML than in the SSW. As a consequence, the higher carbon flux released by lysed cells in the SML than in the SSW would increase the pool of dissolved organic carbon (DOC) and be rapidly used by other prokaryotes to grow (the viral shunt). Thus, our results suggest that viral activity greatly contributes to the functioning of the microbial food web in the SML, which could influence the biogeochemical cycles of the water columnEn prensa

Dysregulation of photosynthetic genes in oceanic Prochlorococcus populations exposed to organic pollutants

The impact of organic pollutants on oceanic ecosystem functioning is largely unknown. Prochlorococcus, the most abundant known photosynthetic organism on Earth, has been suggested to be especially sensible to exposure to organic pollutants, but the sub-lethal effects of organic pollutants on its photosynthetic function at environmentally relevant concentrations and mixtures remain unexplored. Here we show the modulation of the expression of two photosynthetic genes, rbcL (RuBisCO large subunit) and psbA (PSII D1 protein), of oceanic populations of Prochlorococcus from the Atlantic, Indian and Pacific Oceans when exposed to mixtures of organic pollutants consisting of the non-polar fraction of a seawater extract. This mixture included most persistent organic pollutants, semivolatile aromatic-like compounds, and the unresolved complex mixture of hydrocarbons. Prochlorococcus populations in the controls showed the expected diel cycle variations in expression of photosynthetic genes. However, exposure to a complex mixture at concentrations only 2-fold above the environmental levels resulted in a decrease of expression of both genes, suggesting an effect on the photosynthetic function. While organic pollutant effects on marine phytoplankton have been already demonstrated at the cellular level, this is the first field study showing alterations at the molecular level of the photosynthetic function due to organic pollutants.Versión del edito

Severe Deoxygenation Event Caused by the 2011 Eruption of the Submarine Volcano Tagoro (El Hierro, Canary Islands)

The shallow, near-shore submarine volcano Tagoro erupted in October 2011 at the Mar de las Calmas marine reserve, south of El Hierro island. The injection of lava into the ocean had its strongest episode during November 2011 and lasted until March 2012. During this time, in situ measurements of dissolved oxygen were carried out, using a continuous oxygen sensor constantly calibrated with water samples. A severe deoxygenation was observed in the area, particularly during October-November 2011, which was one of the main causes of the high mortality observed among the local marine ecosystem. The measured O2 concentrations were as low as 7.71 µmol kg-1, which represents a -96% decrease with respect to unaffected waters. The oxygen depletion was found in the first 250 m of the water column, with peaks between 70-120 m depth. The deoxygenated plume covered an area of at least 464 km2, distributed particularly south and south-west of the volcano, with occasional patches found north of the island. The oxygen levels were also monitored through the following years, during the degassing stage of the volcano, when oxygen depletion was no longer observed. Additionally, during the eruption, an island-generated anticyclonic eddy interacted with the volcanic plume and transported it for at least 80 km, where the O2 measurements still showed a -8% decrease after mixing and dilution. This feature draws attention to the permanence and transport of volcanic plumes far away from their source and long after the emission.En prens

Viruses and Protists Induced-mortality of Prokaryotes around the Antarctic Peninsula during the Austral Summer

During the Austral summer 2009 we studied three areas surrounding the Antarctic Peninsula: the Bellingshausen Sea, the Bransfield Strait and the Weddell Sea. We aimed to investigate, whether viruses or protists were the main agents inducing prokaryotic mortality rates, and the sensitivity to temperature of prokaryotic heterotrophic production and mortality based on the activation energy (Ea) for each process. Seawater samples were taken at seven depths (0.1–100 m) to quantify viruses, prokaryotes and protists abundances, and heterotrophic prokaryotic production (PHP). Viral lytic production, lysogeny, and mortality rates of prokaryotes due to viruses and protists were estimated at surface (0.1–1 m) and at the Deep Fluorescence Maximum (DFM, 12–55 m) at eight representative stations of the three areas. The average viral lytic production ranged from 1.0 ± 0.3 × 107 viruses ml−1 d−1 in the Bellingshausen Sea to1.3 ± 0.7 × 107 viruses ml−1 d−1 in the Bransfield Strait, while lysogeny, when detectable, recorded the lowest value in the Bellingshausen Sea (0.05 ± 0.05 × 107 viruses ml−1 d−1) and the highest in the Weddell Sea (4.3 ± 3.5 × 107 viruses ml−1 d−1). Average mortality rates due to viruses ranged from 9.7 ± 6.1 × 104 cells ml−1 d−1 in the Weddell Sea to 14.3 ± 4.0 × 104 cells ml−1 d−1 in the Bellingshausen Sea, and were higher than averaged grazing rates in the Weddell Sea (5.9 ± 1.1 × 104 cells ml−1 d−1) and in the Bellingshausen Sea (6.8 ± 0.9 × 104 cells ml−1 d−1). The highest impact on prokaryotes by viruses and main differences between viral and protists activities were observed in surface samples: 17.8 ± 6.8 × 104 cells ml−1 d−1 and 6.5 ± 3.9 × 104 cells ml−1 d−1 in the Weddell Sea; 22.1 ± 9.6 × 104 cells ml−1 d−1 and 11.6 ± 1.4 × 104 cells ml−1 d−1 in the Bransfield Strait; and 16.1 ± 5.7 × 104 cells ml−1 d−1 and 7.9 ± 2.6 × 104 cells ml−1 d−1 in the Bellingshausen Sea, respectively. Furthermore, the rate of lysed cells and PHP showed higher sensitivity to temperature than grazing rates by protists. We conclude that viruses were more important mortality agents than protists mainly in surface waters and that viral activity has a higher sensitivity to temperature than grazing rates. This suggests a reduction of the carbon transferred through the microbial food-web that could have implications in the biogeochemical cycles in a future warmer ocean scenario.En prens

Significant Release of Dissolved Inorganic Nutrients From the Shallow Submarine Volcano Tagoro (Canary Islands) Based on Seven-Year Monitoring

Tagoro, the shallow submarine volcano that erupted south of El Hierro (Canary Islands, Spain) in October 2011, has been intensely monitored for over 7 years, from the early eruptive stage to the current degassing stage characterized by moderate hydrothermal activity. Here, we present a detailed study of the emissions of inorganic macronutrients (NO2– + NO3–, PO4, and Si(OH)4) comprising a dataset of over 3300 samples collected through three different sampling methodologies. Our results show a significant nutrient enrichment throughout the whole studied period, up to 8.8-fold (nitrate), 4.0-fold (phosphate), and 16.3-fold (silicate) in the water column, and larger enrichments of phosphate (10.5-fold) and silicate (325.4-fold), but not of nitrate, in the samples collected directly from the vents. We also provide some preliminary results showing ammonium (NH4+) concentrations up to 1.97 μM in the vent fluids as compared to 0.02 μM in the surrounding waters. Nutrient fluxes from the volcano during the degassing stage were estimated as 3.19 ± 1.17 mol m–2 year–1 (NO2– + NO3–), 0.02 ± 0.01 mol m–2 year–1 (PO4), and 0.60 ± 1.35 mol m–2 year–1 (Si(OH)4), comparable to other important nutrient sources in the region such as fluxes from the NW-African upwelling. Nutrient ratios were affected, with a minimum (NO3– + NO2–):PO4 ratio of 2.36:1; moreover, a linear correlation between silicate and temperature enabled the use of this nutrient as a mixing tracer. This study sheds light on how shallow hydrothermal systems impact the nutrient-poor upper waters of the ocean.En prens

Abundance and Structure of the Zooplankton Community During a Post-eruptive Process: The Case of the Submarine Volcano Tagoro (El Hierro; Canary Islands), 2013-2018

The mesozooplankton community was analyzed over a 6-year period (2013-2018) during the post-eruptive stage of the submarine volcano Tagoro, located south of the island of El Hierro (Canary Archipelago, Spain). Nine cruises from March 2013 to March 2018 were carried out in two different seasons, spring (March-April) and autumn (October). A high-resolution study was carried out across the main cones of Tagoro volcano, as well as a large number of reference stations surrounding El Hierro (unaffected by the volcano). The zooplankton community at the reference stations showed a high similarity with more than 85% of the variation in abundance and composition attributable to seasonal differences. Moreover, our data showed an increase in zooplankton abundance in waters affected by the volcano with a higher presence of non-calanoid copepods and a decline in the diversity of the copepod community, indicating that volcanic inputs have a significant effect on these organisms. Fourteen different zooplankton groups were found but copepods were dominant (79%) with 59 genera and 170 species identified. Despite the high species number, less than 30 presented a larger abundance than 1%. Oncaea and Clausocalanus were the most abundant genera followed by Oithona and Paracalanus (60%). Nine species dominated (>2%): O. media, O. plumifera, and O. setigera among the non-calanoids and M. clausi, P. nanus, P. parvus, C. furcatus, C. arcuicornis, and N. minor among the calanoids. After the initial low abundance of the copepods as a consequence of the eruption, an increase was observed in the last years of the study, where besides the small Paracalanus and Clausocalanus, the Cyclopoids seem to have a good adaptive strategy to the new water conditions. The increase in zooplankton abundance and the decline in the copepod diversity in the area affected by the volcano indicate that important changes in the composition of the zooplankton community have occurred. The effect of the volcanic emissions on the different copepods was more evident in spring when the water was cooler and the mixing layer was deeper. Further and longer research is recommended to monitor the zooplankton community in the natural laboratory of the Tagoro submarine volcano.En prens

Recent Trends in SST, Chl-a, Productivity and Wind Stress in Upwelling and Open Ocean Areas in the Upper Eastern North Atlantic Subtropical Gyre

The global upper ocean has been warming during the last decades accompanied with a chlorophyll-a (Chl-a) and productivity decrease. Whereas subtropical gyres show similar trends, Eastern Boundary Upwelling Systems are thought to increase in productivity due to increased trade winds. This study analyzes recent trends in sea surface temperature (SST), Chl-a, net primary production (NPP) and meridional wind stress in the Eastern North Atlantic subtropical gyre (NASE) in order to examine if the global trends can be detected in open ocean and upwelling areas and how the ocean biota responds. Satellite data of such variables of the last 15–40 years were analyzed to calculate mean trends in upwelling areas in the Canary upwelling system and open ocean areas around the Azores, Madeira and the Canary Islands. Our results show significant warming in the area with a maximum of 2.7°C per century for the Azores. Moreover, a general decreasing trend for Chl-a and NPP seems to be more evident in the permanent upwelling areas, which will be responsible for a loss of 0.13% of the global NPP per century. Our results also highlight a significant expansion of the oceanic desert area of 10% with an increase in unproductive days of up to 84 days in the last 20 years. The competitive relationship of stratification and wind stress in the Canary upwelling system might be a more plausible explanation for the decrease in Chl-a and NPP in upwelling areas linked to the increase in upwelling favorable wind stress and the surface warming.En prens