Role of Individual Disulfide Bonds in the Structural Maturation of a Low Molecular Weight Glutenin Subunit

Gliadins and glutenins are the major storage proteins that accumulate in wheat endosperm cells during seed development. Although gliadins are mainly monomeric, glutenins consist of very large disulfide-linked polymers made up of high molecular weight and low molecular weight subunits. These polymers are among the largest protein molecules known in nature and are the most important determinants of the viscoelastic properties of gluten. As a first step toward the elucidation of the folding and assembly pathways that lead to glutenin polymer formation, we have exploited an in vitro system composed of wheat germ extract and bean microsomes to examine the role of disulfide bonds in the structural maturation of a low molecular weight glutenin subunit. When conditions allowing the formation of disulfide bonds were established, the in vitro synthesized low molecular weight glutenin subunit was recovered in monomeric form containing intrachain disulfide bonds. Conversely, synthesis under conditions that did not favor the formation of disulfide bonds led to the production of large aggregates from which the polypeptides could not be rescued by the post-translational generation of a more oxidizing environment. These results indicate that disulfide bond formation is essential for the conformational maturation of the low molecular weight glutenin subunit and suggest that early folding steps may play an important role in this process, allowing the timely pairing of critical cysteine residues. To determine which cysteines were important to maintain the protein in monomeric form, we prepared a set of mutants containing selected cysteine to serine substitutions. Our results show that two conserved cysteine residues form a critical disulfide bond that is essential in preventing the exposure of adhesive domains and the consequent formation of aberrant aggregates

Analisi multitemporale dell’uso del suolo della dorsale appenninica marchigiana nel XIX e XX secolo

Questo studio ha permesso di ricostruire, per la prima volta nell’Appennino Umbro-Marchigiano, l’evoluzione temporale dell’uso del suolo lungo un arco di tempo di 150 anni e su una superficie di vasta estensione (circa 8000 ettari). Il dato principale che emerge è la sostanziale discontinuità ecologica di tutti gli ecosistemi naturali e seminaturali, fatte salve alcune importanti patches pascolive e, in misura molto minore, boschive. In totale neanche il 20% del territorio dell’area considerata è rimasto stabile nel corso degli ultimi 150 anni; queste aree rappresentano, dal punto di vista floristico, dei potenziali siti di rifugio per le specie erbacee di prateria e per quelle sciafile di bosco. Si tratta quindi di siti estremamente importanti per la conservazione della biodiversità e serbatoi genetici da cui può ripartire la colonizzazione degli ambienti antropizzati e non più utilizzati come le aree agricole abbandonate. È interessante annotare, ad esempio, come proprio in corrispondenza della più ampia patch forestale stabile (Monte Fiegni) sia presente una delle più importanti stazioni dell’Appennino Umbro-Marchigiano di tasso (Taxus baccata) ed agrifoglio (Ilex aquifolium), all’interno di una faggeta basso-montana. Si evidenzia, inoltre, una tendenza alla semplificazione del mosaico ambientale di queste aree montane. Tale semplificazione risulta bene evidente per gli ecosistemi di prateria (stabili e neoformati) che tendono a conservarsi prioritariamente all’interno delle classi di pendenza minore. Questo fenomeno riassume in se il senso dei mutati input che l’economia contemporanea offre alle attività zootecniche; nel corso dei secoli, infatti, si è passati da forme di economia basate sulla transumanza ovina ad un’attività zootecnica di tipo stanziale e dedita soprattutto all’allevamento bovino. Questa differente tipologia zootecnica richiede habitat diversi e, in particolare per quanto concerne i bovini, sono assolutamente indispensabili praterie di elevata produttività e moderata stasi estiva, condizioni che nell’Appennino si verificano solo in corrispondenza delle aree caratterizzate da morfologie a bassa energia di rilievo e in cui si sono anche conservati suoli profondi. Permanendo queste tendenze socio-economiche, dunque, in futuro tenderanno sostanzialmente a scomparire gli ecosistemi di prateria aperta sostituiti da boschi. Questo, se da un lato rappresenta un aspetto che va nel senso della naturale dinamica degli ecosistemi, dall’altro potrebbe comportare dei problemi per la conservazione della biodiversità floristica e faunistica

syntaxonomy and site ecology of a central italy forest landscape

Syntaxonomy and Site Ecology of a Central Italy Forest LandscapeA phytosociological survey of a woodland located in the central part of Umbria (Central Italy) was carried out using the Braun-Blanquet method: 80 relevés were classified through cluster analysis. Nine forestsyntaxawere reported and three subassociations (Erico arboreae-Quercetum cerridis lathyretosum veneti, Aceri obtusati-Quercetum cerridis arbutetosum unedonisandCyclamino hederifolii-Quercetum ilicis quercetosum cerridis) were typified. Topographic data (altitude, aspect, slope, morphology) and pedological data (soil pH, texture and depth) were collected and a synecological analysis ofsyntaxawas performed. In order to understand the relation between environmental factors and plant communities, a Canonical Correspondence Analysis was run. The results showed soil parameters (pH, texture and depth) and altitude as the main ecological factors explaining the distribution of plant communities in the study area. The combination of topographic factors (aspect, morphology and slope angle) influences, by contrast revealed the distribution of forestsyntaxawithin homogeneous geo-pedological and bioclimatic conditions

Approccio geosinfitosociologico alla definizione della carrying capacity potenziale degli ecosistemi forestali dell’Appennino centrale nei confronti del capriolo (Capreolus capreolus L.)

An intense debate is developed on the opportunity and the possibility to realize a system of representation of the ecosystems as fundamental element for the territorial planning, the sustainable development, the management of the habitats and animal communities. This work is aimed to the definition of the theoretical carrying capacity of the more diffused forest ecosystems in the Province of Macerata by means of the analysis of the edible phytomass from the roe deer in the winter period, corresponding to the vegetative stasis. For the evaluation of such availability, besides the floristic-structural characteristics of the forest ecosystems, it needs to know the length of the vegetative stasis period (related to every ecosystem and correlated to the bioclimatic peculiarities of the territory) as well as of the behavioural aspects in the assumption of food and the anatomical characteristics of alimentary tract of the studied kind. The analysis of the harvested phytomass (inside sample areas related to every forest ecosystem) has shown a notable variability in the quantity of edible phytomass within the considered forest ecosystems, testifying as a notable difference of vocation for the roe deer exists among different ecosystems

The small satellite NINA-MITA to study galactic and solar cosmic rays in low-altitude polar orbit

Abstract The satellite MITA, carrying on board the scientific payload NINA-2, was launched on July the 15th, 2000 from the cosmodrome of Plesetsk (Russia) with a Cosmos-3M rocket. The satellite and the payload are currently operating within nominal parameters. NINA-2 is the first scientific payload for the technological flight of the Italian small satellite MITA. The detector used in this mission is identical to the one already flying on the Russian satellite Resurs-O1 n.4 in a 840-km sun-synchronous orbit, but makes use of the extensive computer and telemetry capabilities of MITA bus to improve the active data acquisition time. NINA physics objectives are to study cosmic nuclei from hydrogen to iron in the energy range between 10 MeV/n and 1 GeV/n during the years 2000–2003, that is the solar maximum period. The device is capable of charge identification up to iron with isotope sensitivity up to oxigen. The 87.3 degrees, 460 km altitude polar orbit allows investigations of cosmic rays of solar and galactic origin, so to study long and short term solar transient phenomena, and the study of the trapped radiation at higher geomagnetic cutoff

THE SPACE TELESCOPE NINA: RESULTS OF A BEAM TEST CALIBRATION

Abstract In June 1998 the telescope NINA will be launched in space on board of the Russian satellite Resource-01 n.4. The main scientific objective of the mission is the study of the anomalous, galactic and solar components of the cosmic rays in the energy interval 10–200 MeV/n. The core of the instrument is a silicon detector whose performances have been tested with a particle beam at the GSI Laboratory in Germany in 1997; we report here on the results obtained during the beam calibration

Gamma-Ray Burst observations by the high-energy charged particle detector on board the CSES-01 satellite between 2019 and 2021

In this paper we report the detection of five strong Gamma-Ray Bursts (GRBs) by the High-Energy Particle Detector (HEPD-01) mounted on board the China Seismo-Electromagnetic Satellite (CSES-01), operational since 2018 on a Sun-synchronous polar orbit at a $\sim$ 507 km altitude and 97$^\circ$ inclination. HEPD-01 was designed to detect high-energy electrons in the energy range 3 - 100 MeV, protons in the range 30 - 300 MeV, and light nuclei in the range 30 - 300 MeV/n. Nonetheless, Monte Carlo simulations have shown HEPD-01 is sensitive to gamma-ray photons in the energy range 300 keV - 50 MeV, even if with a moderate effective area above $\sim$ 5 MeV. A dedicated time correlation analysis between GRBs reported in literature and signals from a set of HEPD-01 trigger configuration masks has confirmed the anticipated detector sensitivity to high-energy photons. A comparison between the simultaneous time profiles of HEPD-01 electron fluxes and photons from GRB190114C, GRB190305A, GRB190928A, GRB200826B and GRB211211A has shown a remarkable similarity, in spite of the different energy ranges. The high-energy response, with peak sensitivity at about 2 MeV, and moderate effective area of the detector in the actual flight configuration explain why these five GRBs, characterised by a fluence above $\sim$ 3 $\times$ 10$^{-5}$ erg cm$^{-2}$ in the energy interval 300 keV - 50 MeV, have been detected.Comment: Accepted for publication in The Astrophysical Journal (ApJ