Doctor of Philosophy

dissertationParkinson's Disease (PD) motor symptoms, characterized most commonly by bradykinesia, akinesia, rigidity, and tremor, are brought about through the degeneration of dopaminergic neurons in the substantia nigra pars compacta, which leads to changes in electrophysiological activity throughout the basal ganglia. These symptoms are often effectively treated in the early stages of the disease by dopamine replacement therapies. However, as the disease progresses, the therapeutic window of pharmacological approaches reduces and patients develop significant side effects, even under minimally effective doses. When the disease reaches this stage, surgical therapies, such as high-frequency deep brain stimulation (DBS), are considered. DBS of the subthalamic nucleus partially treats the motor symptoms of PD and has been implemented to treat PD over 50,000 times worldwide, but its mechanisms are unclear. In this work, we set out to advance the understanding of the mechanisms, function, and malfunction of DBS as a treatment for PD, keeping in mind the idea that DBS treats PD symptoms without restoring basal ganglia neural activity to that seen under healthy conditions. First, we demonstrated that neuronal information directed from the basal ganglia to the thalamus is pathologically increased in the parkinsonian condition and reduced by DBS in a standard 6-OHDA rat model of PD. Next, we developed a rodent model of DBSs role in the exacerbation of hypokinetic dysarthria, providing a framework for the study of this poorly understood side effect of DBS. Finally, we found that DBS creates action suppression deficits independently from a parkinsonian state, and that PD creates apathy that is not rescued by DBS. Our specific results led to the interpretation that DBS, in its current form, might inherently create side effects that are largely unavoidable. Our work fits into the following overarching idea. DBS successfully treats some motor symptoms of PD through the reduction of pathological information transmission. However, the fact that reducing pathological information does not restore neural activity to that present under healthy conditions underlies some of its failures to improve certain symptoms, as well as its exacerbations and side effects

Assessing Prescriber and Staff Readiness for Change and Evaluation of Chronic Pain Management

Survey-based study of opioid prescribers and staff members at a community health center. Designed to assess current opioid prescribing practices, management of chronic pain patients, and readiness for future change.https://scholarworks.uvm.edu/fmclerk/1629/thumbnail.jp

Influence of Woody Vegetation Composition and Structure on Fuels and Prescribed Fire in Mountain Longleaf Restoration

Longleaf pine (LLP) ecosystems have experienced a widespread ecological state shift largely due to fire exclusion which has allowed mesophytes, i.e., shade-tolerant, often fire-sensitive species to encroach, reducing flammability and biodiversity through a process known as “mesophication.” Although prescribed fire is commonly used to reverse mesophication, fire behavior, and thus prescribed fire utility for this purpose, is poorly characterized in mixed pine-hardwood stands with mesophyte encroachment. This study aimed to identify mechanisms by which tree composition, structure, and fuels contribute to fire behavior, focusing on the understudied mountain longleaf pine (MLLP) ecoregion in northwest Georgia. I hypothesized that woody vegetation composition and structure indirectly influence fire behavior through fuel bed traits. Relative decreases in basal area and increases in the relative importance of pine and pyrophytic hardwoods (e.g., Quercus spp.) were expected to increase fuel load and reduce bulk density, thereby increasing fire rate of spread (RoS), fuel consumption, and residence time. To test this, I collected fuel and fire data across a gradient of woody vegetation composition and structure during dormant season prescribed burns and used Bayesian path analysis to estimate the effects of vegetation, fuel traits, and weather on fire. Results showed that the canopy directly influenced fire behavior but lacked significant indirect effects through fuel bed traits. Despite lacking a significant relationship with the canopy, greater fuel bed bulk density significantly reduced RoS and duff consumption. Precise mechanisms remain unresolved, but increased mesophyte importance directly and indirectly reduced fire intensity, in part by reducing available fuel load, and should be targeted for removal in restoration efforts. In contrast, pyrophytic hardwoods significantly increased fire intensity in MLLP

Citation Filtered: Iran’s Censorship of Wikipedia

Using proxy servers in Iran, researchers Collin Anderson and Nima Nazeri identified every blocked Persian language Wikipedia article and divided blocked pages into ten categories to determine the type of content state censors are most adverse. In total, 963 blocked articles were found, covering a range of socio-political and sexual content including politics, journalism, the arts, religion, sex, sexuality, and human rights. Censors repeatedly targeted Wikipedia pages about government rivals, minority religious beliefs, and criticisms of the state, officials, and the police. Just under half of the blocked Wiki-pages are biographies, including pages about individuals the authorities have allegedly detained or killed. Based on prior research, it is known that Iran’s Internet filtration relies on blacklists of specifically designated URLs and URL keywords. Keyword filtration blindly blocks pages that contain prohibited character patterns in the URL. Sexual content is the main target of keywords, for example most keywords are sexual and/or profane terms. We found dozens of pages that seem to be unintentionally censored by keyword filtering, meaning that they were misidentified as sexual or profane and contained no content likely to offend Iranian authorities

Liquidus temperature and optical properties measurement by containerless techniques

Reactive alloy liquidus temperatures measured by conventional, contained techniques are often in error due to reactions with containers and gaseous impurities. This paper describes a new liquidus temperature measurement technique that avoids these problems by employing containerless processing. This technique relies on precise and accurate noncontact temperature measurements (NCTM), which are made possible by spectral emissivity values. The spectral emissivities, epsilon(sub lambda), are measured along with the optical properties (real, n, and imaginary, k, components of the index of refraction) using polarimetric techniques on electromagnetically levitated specimens. Results from work done at Vanderbilt University and Intersonics on the Ti-Al system are presented to demonstrate the above techniques

Containerless high temperature property measurements

Containerless processing in the low gravity environment of space provides the opportunity to increase the temperature at which well controlled processing of and property measurements on materials is possible. This project was directed towards advancing containerless processing and property measurement techniques for application to materials research at high temperatures in space. Containerless high temperature material property studies include measurements of the vapor pressure, melting temperature, optical properties, and spectral emissivities of solid boron. The reaction of boron with nitrogen was also studied by laser polarimetric measurement of boron nitride film growth. The optical properties and spectral emissivities were measured for solid and liquid silicon, niobium, and zirconium; liquid aluminum and titanium; and liquid Ti-Al alloys of 5 to 60 atomic pct. titanium. Alternative means for noncontact temperature measurement in the absence of material emissivity data were evaluated. Also, the application of laser induced fluorescence for component activity measurements in electromagnetic levitated liquids was studied, along with the feasibility of a hybrid aerodynamic electromagnetic levitation technique

The WTO’s Revised Government Procurement Agreement - An Important Milestone Toward Greater Market Access and Transparency in Global Public Procurement Markets

In December of 2011, the Parties to the World Trade Organization Government Procurement Agreement (GPA) adopted significant revisions to the Agreement. The revised Agreement comprises (a) a much-needed modernization of the text of the Agreement, (b) an expansion of related market-access commitments by the Parties, and (c) a set of Future Work Programs intended to enhance transparency among the Parties and improve the administration of the Agreement. In these unstable economic times, the importance of the GPA and its improvements cannot be overstated.This article also bemoans the media\u27s misrepresentation of the ongoing process of China\u27s negotiated accession into the GPA. China continues to invest resources in upgrading its public procurement regime as it negotiates with the GPA Parties to open its government purchasing markets to systematic international competition

Bovine Rhinitis Viruses Are Common in US Cattle with Bovine Respiratory Disease

Citation: Hause, B. M., Collin, E. A., Anderson, J., Hesse, R. A., & Anderson, G. (2015). Bovine Rhinitis Viruses Are Common in US Cattle with Bovine Respiratory Disease. Plos One, 10(3), 12. doi:10.1371/journal.pone.0121998Bovine rhinitis viruses (BRV) are established etiological agents of bovine respiratory disease complex however little research into their epidemiology and ecology has been published for several decades. In the U.S., only bovine rhinitis A virus 1 (BRAV1) has been identified while bovine rhinitis A virus 2 (BRAV2) and bovine rhinitis B virus (BRBV) were previously only identified in England and Japan, respectively. Metagenomic sequencing of a nasal swab from a bovine respiratory disease (BRD) diagnostic submission from Kansas identified contigs with approximately 90% nucleotide similarity to BRAV2 and BRBV. A combination of de novo and templated assemblies using reference genomes yielded near complete BRAV2 and BRBV genomes. The near complete genome of bovine rhinitis A virus 1 (BRAV1) was also determined from a historical isolate to enable further molecular epidemiological studies. A 5'-nuclease reverse transcription PCR assay targeting the 3D polymerase gene was designed and used to screen 204 archived BRD clinical specimens. Thirteen (6.4%) were positive. Metagenomic sequencing of six positive samples identified mixed BRAV1/BRAV2, BRAV1/BRBV and BRAV2/BRBV infections for five samples. One sample showed infection only with BRAV1. Seroprevalence studies using a cell culture adapted BRBV found immunofluorescence assay-reactive antibodies were common in the herds analyzed. Altogether, these results demonstrate that BRV infections are common in cattle with respiratory disease and that BRAV1, BRAV2 and BRBV co-circulate in U.S. cattle and have high similarity to viruses isolated more than 30 years ago from diverse locations