726 research outputs found
Chiral transition in a magnetic field and at finite baryon density
We consider the quark-meson model with two quark flavors in a constant
external magnetic field at finite temperature and finite baryon
chemical potential . We calculate the full renormalized effective
potential to one-loop order in perturbation theory. We study the system in the
large- limit, where we treat the bosonic modes at tree level. It is shown
that the system exhibits dynamical chiral symmetry breaking, i. e. that an
arbitrarily weak magnetic field breaks chiral symmetry dynamically, in
agreement with earlier calculations using the NJL model. We study the influence
on the phase transition of the fermionic vacuum fluctuations. For strong
magnetic fields, and in the chiral limit, the transition
is first order in the entire plane if vacuum fluctuations are not
included and second order if they are included. At the physical point, the
transition is a crossover for with and without vacuum fluctuations.Comment: 11 pages. 5figs. V2: fixed a few typos and added refs. Submitted to
PRD. V3: Added refs and substantial revision of tex
Post-transcriptional regulation of satellite cell quiescence by TTP-mediated mRNA decay.
Skeletal muscle satellite cells in their niche are quiescent and upon muscle injury, exit quiescence, proliferate to repair muscle tissue, and self-renew to replenish the satellite cell population. To understand the mechanisms involved in maintaining satellite cell quiescence, we identified gene transcripts that were differentially expressed during satellite cell activation following muscle injury. Transcripts encoding RNA binding proteins were among the most significantly changed and included the mRNA decay factor Tristetraprolin. Tristetraprolin promotes the decay of MyoD mRNA, which encodes a transcriptional regulator of myogenic commitment, via binding to the MyoD mRNA 3' untranslated region. Upon satellite cell activation, p38α/β MAPK phosphorylates MAPKAP2 and inactivates Tristetraprolin, stabilizing MyoD mRNA. Satellite cell specific knockdown of Tristetraprolin precociously activates satellite cells in vivo, enabling MyoD accumulation, differentiation and cell fusion into myofibers. Regulation of mRNAs by Tristetraprolin appears to function as one of several critical post-transcriptional regulatory mechanisms controlling satellite cell homeostasis
Construction of a multiple fluorescence labelling system for use in co-invasion studies of Listeria monocytogenes
BACKGROUND: Existing virulence models are often difficult to apply for quantitative comparison of invasion potentials of Listeria monocytogenes. Well-to-well variation between cell-line based in vitro assays is practically unavoidable, and variation between individual animals is the cause of large deviations in the observed capacity for infection when animal models are used. One way to circumvent this problem is to carry out virulence studies as competition assays between 2 or more strains. This, however, requires invasion-neutral markers that enable easy discrimination between the different strains. RESULTS: A fluorescent marker system, allowing visualization and identification of single L. monocytogenes cells as well as colonies in a non-destructive manner, was developed. Five different fluorescent labels are available, and allowed simultaneous visual discrimination between three differently labelled strains at the single cell level by use of fluorescence microscopy. More than 90% of the L. monocytogenes host cells maintained the fluorescence tags for 40 generations. The fluorescence tags did not alter the invasive capacity of the L. monocytogenes cells in a traditional Caco-2 cell invasion assay, and visual discrimination between invaded bacteria carrying different fluorescent labels inside the cells was possible. CONCLUSION: The constructed fluorescent marker system is stable, easy to use, does not affect the virulence of L. monocytogenes in Caco-2 cell assays, and allows discrimination between differently labelled bacteria after internalization in these cells
Mass Expansions of Screened Perturbation Theory
The thermodynamics of massless phi^4-theory is studied within screened
perturbation theory (SPT). In this method the perturbative expansion is
reorganized by adding and subtracting a mass term in the Lagrangian. We
analytically calculate the pressure and entropy to three-loop order and the
screening mass to two-loop order, expanding in powers of m/T. The truncated
m/T-expansion results are compared with numerical SPT results for the pressure,
entropy and screening mass which are accurate to all orders in m/T. It is shown
that the m/T-expansion converges quickly and provides an accurate description
of the thermodynamic functions for large values of the coupling constant.Comment: 22 pages, 10 figure
Reduced energy intake was the main contributing factor to weight loss maintenance three years after gastric bypass
Screened Perturbation Theory to Three Loops
The thermal physics of a massless scalar field with a phi^4 interaction is
studied within screened perturbation theory (SPT). In this method the
perturbative expansion is reorganized by adding and subtracting a mass term in
the lagrangian. We consider several different mass prescriptions that
generalize the one-loop gap equation to two-loop order. We calculate the
pressure and entropy to three-loop order and the screening mass to two-loop
order. In contrast to the weak-coupling expansion, the SPT-improved
approximations appear to converge even for rather large values of the coupling
constant.Comment: 30 pages, 10 figure
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