Is the Bucharest Convention on the protection of the Black Sea against pollution still a matter of general concern? Has it reached its purpose?

If one takes into account the challenges that the Black Sea faces - eutrophication, chemical pollution, threats to biodiversity and dramatic reduction of living marine resources, mainly due to the inappropriate conservation measures regarding the fishing areas at the maritime basis - the question that arises is whether we can assert -after 20 years of application - that the Bucharest Convention on the protection of the Black Sea against pollution is still useful, that it has reached its purpose. The question is favored by the fact that the European Union seems more and more concerned with cooperation in the area, which also results from the former Communication of the Commission "Black Sea Synergy - a New Regional Cooperation Initiative", COM(2007)160.chemical pollution, threats to biodiversity

AGRIMONY AND CICHORY - SOURCES OF SAPONINS

Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”, Chişinău, Republica MoldovaIntroducere. Saponozidele sunt produși ai metabolismului secundar care se remarcă prin multiple activități farmacologice: antimicrobiene, antivirale, antioxidante, imunomodulatoare, iar plantele pot servi ca surse de materie primă locală pentru noi produse farmaceutice. Scopul lucrării. Identificarea și dozarea saponozidelor din produsele vegetale: Agrimoniae herba și Cichorii herba. Material și Metode. Părțile aeriene de turiță (Agrimonia eupatoria L.) și cicoare (Cichorium intybus L.) au fost recoltate în perioada de înflorire, din colecția Centrului Științifico Practic în Domeniul Plantelor Medicinale a USMF ”Nicolae Testemițanu”. Dozarea saponozidelor s-a realizat cu ajutorul spectrofotometrului Metertech UV/VIS SP 8001 la lungimea de undă λ = 540, conform metodei cu vanilină-acid sulfuric. Rezultate. Prezența saponozidelor în părțile aeriene ale produselor vegetale menționate a fost determinată prin cromatografia pe strat subțire, cu un Rf=0,32, comparativ cu saponina standard. Din saponozide, în produsul vegetal Agrimoniae herba se conțin acizii euscapic, ursolic și tormentic, iar în Cichorii herba au fost identificate α-amirina, α-lactucerolul și taraxerona. Analiza fitochimică realizată prin metoda spectrofotometrică conform testului vanilină-acid sulfuric a evidențiat prezența saponozidelor cu o concentrație de 371,053 mg/L pentru Agrimoniae herba și 216,191 mg/L pentru Cichorii herba. Concluzii. Produsele vegetale recoltate de la speciile: A. eupatoria și C. intybus din colecția CȘPDPM pot fi utilizate ca surse de saponozide și de noi forme farmaceutice.Background. Saponosides are products of secondary metabolism that are noted for their multiple pharmacological activities: antimicrobial, antiviral, antioxidant and immunomodulatory, and the plants can serve as local raw material sources for new pharmaceutical products. Objective of the study. Identification and dosage of saponosides in the vegetal products: Agrimoniae herba and Cichorii herba. Material and Methods. The aerial parts of agrimony (Agrimonia eupatoria L.), and cichory (Cichorium intybus L.) were collected in the flowering period, from the collection of Scientific and Practical Centre for Medicinal Plants of the „Nicolae Testemițanu” SUMPh. Dosage of saponosides was realized by Metertech UV/VIS SP 8001 Spectrophotometer, at wavelength of λ = 540, according to the vanillin-sulphuric acid method. Results. By thin layer chromatography, the presence of saponosides in the aerial parts of the mentioned vegetal products was determined with an Rf = 0.32, compared to standard saponin. From saponosides, in Agrimoniae herba vegetal product are contained ursolic, euscapic and tormentic acids, and in Cichorii herba were identified α-amyrin, α-lactucerol and taraxerone. Phytochemical analysis realized by the spectrophotometric method according to the vanillin-sulphuric acid test revealed the presence of saponosides with a concentration of 371.053 mg/L for Agrimoniae herba and 216.191 mg/L for Cichorii herba. Conclusion. Vegetal products harvested from species: A. eupatoria and C. intybus from the collection of SPCMP can be used as sources of saponosides and new pharmaceutical forms

Turița și cicoarea – surse de saponozide

Background. Saponosides are products of secondary metabolism that are noted for their multiple pharmacological activities: antimicrobial, antiviral, antioxidant and immunomodulatory, and the plants can serve as local raw material sources for new pharmaceutical products. Objective of the study. Identification and dosage of saponosides in the vegetal products: Agrimoniae herba and Cichorii herba. Material and Methods. The aerial parts of agrimony (Agrimonia eupatoria L.), and cichory (Cichorium intybus L.) were collected in the flowering period, from the collection of Scientific and Practical Centre for Medicinal Plants of the „Nicolae Testemițanu” SUMPh. Dosage of saponosides was realized by Metertech UV/VIS SP 8001 Spectrophotometer, at wavelength of λ = 540, according to the vanillin-sulphuric acid method. Results. By thin layer chromatography, the presence of saponosides in the aerial parts of the mentioned vegetal products was determined with an Rf = 0.32, compared to standard saponin. From saponosides, in Agrimoniae herba vegetal product are contained ursolic, euscapic and tormentic acids, and in Cichorii herba were identified α-amyrin, α-lactucerol and taraxerone. Phytochemical analysis realized by the spectrophotometric method according to the vanillin- sulphuric acid test revealed the presence of saponosides with a concentration of 371.053 mg/L for Agrimoniae herba and 216.191 mg/L for Cichorii herba. Conclusion. Vegetal products harvested from species: A. eupatoria and C. intybus from the collection of SPCMP can be used as sources of saponosides and new pharmaceutical forms.Study carried out with the support of the project 20.80009.8007.24 “Biological and phytochemical study of medicinal plants with antioxidant, antimicrobial and hepatoprotective action” within the State Program (2020-2023), project manager Ciobanu Nicolae, Ph.D. farm., associate professor, contracting authority: National Agency for Research and Development.Introducere. Saponozidele sunt produși ai metabolismului secundar care se remarcă prin multiple activități farmacologice: antimicrobiene, antivirale, antioxidante, imunomodulatoare, iar plantele pot servi ca surse de materie primă locală pentru noi produse farmaceutice. Scopul lucrării. Identificarea și dozarea saponozidelor din produsele vegetale: Agrimoniae herba și Cichorii herba. Material și Metode. Părțile aeriene de turiță (Agrimonia eupatoria L.) și cicoare (Cichorium intybus L.) au fost recoltate în perioada de înflorire, din colecția Centrului Științifico Practic în Domeniul Plantelor Medicinale a USMF ”Nicolae Testemițanu”. Dozarea saponozidelor s-a realizat cu ajutorul spectrofotometrului Metertech UV/VIS SP 8001 la lungimea de undă λ = 540, conform metodei cu vanilină-acid sulfuric. Rezultate. Prezența saponozidelor în părțile aeriene ale produselor vegetale menționate a fost determinată prin cromatografia pe strat subțire, cu un Rf=0,32, comparativ cu saponina standard. Din saponozide, în produsul vegetal Agrimoniae herba se conțin acizii euscapic, ursolic și tormentic, iar în Cichorii herba au fost identificate α-amirina, α-lactucerolul și taraxerona. Analiza fitochimică realizată prin metoda spectrofotometrică conform testului vanilină-acid sulfuric a evidențiat prezența saponozidelor cu o concentrație de 371,053 mg/L pentru Agrimoniae herba și 216,191 mg/L pentru Cichorii herba. Concluzii. Produsele vegetale recoltate de la speciile: A. eupatoria și C. intybus din colecția CȘPDPM pot fi utilizate ca surse de saponozide și de noi forme farmaceutice.Studiu realizat cu suportul proiectului 20.80009.8007.24„ Studiul biologic și fitochimic al plantelor medicinale cu acțiune antioxidantă, antimicrobiană și hepatoprotectoare” din cadrul Programului de Stat (2020-2023), conducător de proiect Ciobanu Nicolae, dr. șt. farm., conf. univ., autoritatea contractantă: Agenția Națională pentru Cercetare și Dezvoltare

Is the Bucharest Convention on the protection of the Black Sea against pollution still a matter of general concern? Has it reached its purpose?

If one takes into account the challenges that the Black Sea faces eutrophication, chemical pollution, threats to biodiversity and dramatic reduction of living marine resources, mainly due to the inappropriate conservation measures regarding the fishing areas at the maritime basis - the question that arises is whether we can assert after 20 years of application - that the Bucharest Convention on the protection of the Black Sea against pollution is still useful, that it has reached its purpose. The question is favored by the fact that the European Union seems more and more concerned with cooperation in the area, which also results from the former Communication of the Commission Black Sea Synergy a New Regional Cooperation Initiative, COM(2007)160

Chemical profile of saponosides in aerial parts of agrimony and cichory

Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”, Chişinău, Republica MoldovaIntroducere. Saponozidele sunt un grup de compuși vegetali care posedă proprietăți terapeutice antiinflamatoare, antimicrobiene, imunomodulatoare, cicatrizante, iar plantele medicinale pot fi utilizate ca materie primă cu scopul de a obține noi produse farmaceutice. Scopul lucrării. Identificarea și dozarea saponozidelor din produsele vegetale: Agrimoniae herba (Agrimonia eupatoria L.) și Cichorii herba (Cichorium intybus L.). Material și metode. Părțile aeriene de turiță și cicoare au fost recoltate în perioada de înflorire, din colecția CȘPDPM. Saponozidele s-au identificat prin reacții specifice și prin cromatografia pe strat subțire (în 3 sisteme). Dozarea saponozidelor s-a realizat cu ajutorul spectrofotometrului Metertech UV/VIS SP 8001 la lungimea de undă λ = 540 nm. Rezultate. Prin reacții specifice de identificare (cu acetat de plumb, reacția de formare a spumei, Liebermann-Bourchard, Lafon, cu nitrat de sodiu) și prin cromatografia pe strat subțire s-au identificat saponozidele triterpenice în părțile aeriene de turiță și cicoare. Analiza cantitativă realizată prin metoda spectrofotometrică conform testului vanilină-acid sulfuric a evidențiat prezența saponozidelor cu o concentrație de 371,053 mg/L pentru Agrimoniae herba, urmat de 216,19 mg/L pentru Cichorii herba, în recalcul după curba de etalonare pentru saponozidă (1 mg/ml; y = 0,0008X+0,0081; R = 0,9976). Concluzii. Părțile aeriene recoltate de la speciile: Agrimonia eupatoria și Cichorium intybus din colecția CȘPDPM pot servi ca surse de saponozide în scopul elaborării de noi forme farmaceutice.Background. Saponosides are a group of plant compounds that possess anti-inflammatory, antimicrobial, immunomodulatory, cicatrizing therapeutic properties, therefore the plants could be used as raw material in order to obtain new pharmaceutical products. Objective of the study. Identification and dosage of saponosides in the vegetal products: Agrimoniae herba and Cichorii herba. Material and methods. The aerial parts of agrimony and cichory were collected in the flowering period, from the collection of Scientific and Practical Center for Medicinal Plants of the Nicolae Testemițanu University. Saponosides were identified through specific reactions and by thin layer chromatography (in 3 systems). Dosage of saponosides was realized by Metertech UV/VIS SP 8001 Spectrophotometer, at wavelength of λ = 540 nm. Results. By specific identification reactions (with lead acetate, the foaming reaction, Liebermann-Bourchard, Lafon, with sodium nitrate) and by thin layer chromatography, triterpene saponosides were identified in the aerial parts of agrimony and chicory. The quantitative analysis carried out by the spectrophotometric method according to the vanillin-sulfuric acid test revealed the presence of saponosides with a concentration of 371.053 mg/L for Agrimoniae herba, followed by 216.19 mg/L for Cichorii herba, in recalculation after the calibration curve for saponoside (1 mg/ml; y = 0.0008X+0.0081; R = 0.9976). Conclusion. Aerial parts harvested from species: Agrimonia eupatoria and Cichorium intybus from the collection of SPCMP can be used as sources of saponosides and new pharmaceutical forms

Ultralight vector dark matter search using data from the KAGRA O3GK run

Among the various candidates for dark matter (DM), ultralight vector DM can be probed by laser interferometric gravitational wave detectors through the measurement of oscillating length changes in the arm cavities. In this context, KAGRA has a unique feature due to differing compositions of its mirrors, enhancing the signal of vector DM in the length change in the auxiliary channels. Here we present the result of a search for U(1)B−L gauge boson DM using the KAGRA data from auxiliary length channels during the first joint observation run together with GEO600. By applying our search pipeline, which takes into account the stochastic nature of ultralight DM, upper bounds on the coupling strength between the U(1)B−L gauge boson and ordinary matter are obtained for a range of DM masses. While our constraints are less stringent than those derived from previous experiments, this study demonstrates the applicability of our method to the lower-mass vector DM search, which is made difficult in this measurement by the short observation time compared to the auto-correlation time scale of DM

Search for eccentric black hole coalescences during the third observing run of LIGO and Virgo

Despite the growing number of confident binary black hole coalescences observed through gravitational waves so far, the astrophysical origin of these binaries remains uncertain. Orbital eccentricity is one of the clearest tracers of binary formation channels. Identifying binary eccentricity, however, remains challenging due to the limited availability of gravitational waveforms that include effects of eccentricity. Here, we present observational results for a waveform-independent search sensitive to eccentric black hole coalescences, covering the third observing run (O3) of the LIGO and Virgo detectors. We identified no new high-significance candidates beyond those that were already identified with searches focusing on quasi-circular binaries. We determine the sensitivity of our search to high-mass (total mass M>70 M⊙) binaries covering eccentricities up to 0.3 at 15 Hz orbital frequency, and use this to compare model predictions to search results. Assuming all detections are indeed quasi-circular, for our fiducial population model, we place an upper limit for the merger rate density of high-mass binaries with eccentricities 0<e≤0.3 at 0.33 Gpc−3 yr−1 at 90\% confidence level

WHY USING THE STAMP TECHNIQUE IN CLASS I CAVITIES IS A FIRST CHOICE FILLING TECHNIQUE

Restoring lateral teeth with composite is always a challenge for the dentist as the morphology can be quite tricky to restore and the importance of making it perfect is crucial, not for esthetic reason but for clinical reasons, as a balanced occlusion is very important to keep. The Stamp technique helps the doctor to make the occlusal surface as it was originally, due to an impression taken of the natural morphology and replicating it on the composite filling that is done. The technique is time and cost efficient, with great clinical outcome

First Molecular Data of <i>Gongylonema pulchrum</i> (Rhabditida: Gongylonematidae) in European Fallow Deer <i>Dama dama</i> from Romania

Due to its adaptive versatility to numerous types of habitats, extremely diverse both in terms of composition and specificity, developed in various areas of the Western Plains of Romania, the European fallow deer (Dama dama) is a species with high ecological plasticity. In this area, the D. dama interacts with other species of wild fauna but also with numerous domestic animals, an important aspect in terms of the sanitary-veterinary status of animal populations, as well as the existence of a potential risk of infection with various species of parasites that can cause the D. dama specimens to obtain certain diseases and even zoonoses. A total of 133 esophagi from D. dama have been examined for helminths. Of the 133 esophagus samples from D. dama, nematodes of the genus Gongylonema were identified in 25 (18.80%). Sequencing revealed that the nematode identified in the samples was 99% similar to the sequence of Gongylonema pulchrum (GenBank no. LC026018.1, LC388754.1, AB646061). The present research is the first report of the nematode G. pulchrum from D. dama in Romania