353 research outputs found
Identification and validation of signature for prognosis and immune microenvironment in gastric cancer based on m6A demethylase ALKBH5
BackgroundN6-methyladenosine (m6A) RNA regulators play important roles in cancers, but their functions and mechanism have not been demonstrated clearly in gastric cancer (GC).MethodsIn this study, the GC samples with clinical information and RNA transcriptome were downloaded from The Cancer Genome Atlas database. The different expression genes were compared by the absolute value and median ± standard deviation. Samples with complete information were randomly divided into a training dataset and a test dataset. The differential expression genes (DEGs) between ALKBH5-low and ALKBH5-high subgroups were identified in the training dataset and constructed a risk model by Cox and least absolute shrinkage and selection operator regression. The model was testified in test datasets, overall survival (OS) was compared with the KaplanâMeier method, and immune cell infiltration was calculated by the CIBERSORT algorithm in the low-risk and high-risk subgroups based on the model. The protein levels of ALKBH5 were detected with immunohistochemistry. The relative expression of messenger-ribonucleic acid (mRNA) was detected with quantitative polymerase chain reaction.ResultsALKBH5 was the only regulator whose expression was lower in tumor samples than that in normal samples. The low expression of ALKBH5 led to the poor OS of GC patients and seemed to be an independent protective factor. The model based on ALKBH5-regulated genes was validated in both datasets (training/test) and displayed a potential capacity to predict a clinical prognosis. Gene Ontology analysis implied that the DEGs were involved in the immune response; CIBERSORT results indicated that ALKBH5 and its related genes could alter the immune microenvironment of GC. The protein levels of ALKBH5 were verified as lowly expressed in GC tissues. SLC7A2 and CGB3 were downregulated with ALKBH5 knockdown.ConclusionsIn this study, we found that ALKBH5 might be a suppressor of GC; ALKBH5 and its related genes were latent biomarkers and immunotherapy targets
Multidifferential study of identified charged hadron distributions in -tagged jets in proton-proton collisions at 13 TeV
Jet fragmentation functions are measured for the first time in proton-proton
collisions for charged pions, kaons, and protons within jets recoiling against
a boson. The charged-hadron distributions are studied longitudinally and
transversely to the jet direction for jets with transverse momentum 20 GeV and in the pseudorapidity range . The
data sample was collected with the LHCb experiment at a center-of-mass energy
of 13 TeV, corresponding to an integrated luminosity of 1.64 fb. Triple
differential distributions as a function of the hadron longitudinal momentum
fraction, hadron transverse momentum, and jet transverse momentum are also
measured for the first time. This helps constrain transverse-momentum-dependent
fragmentation functions. Differences in the shapes and magnitudes of the
measured distributions for the different hadron species provide insights into
the hadronization process for jets predominantly initiated by light quarks.Comment: All figures and tables, along with machine-readable versions and any
supplementary material and additional information, are available at
https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-013.html (LHCb
public pages
Study of the decay
The decay is studied
in proton-proton collisions at a center-of-mass energy of TeV
using data corresponding to an integrated luminosity of 5
collected by the LHCb experiment. In the system, the
state observed at the BaBar and Belle experiments is
resolved into two narrower states, and ,
whose masses and widths are measured to be where the first uncertainties are statistical and the second
systematic. The results are consistent with a previous LHCb measurement using a
prompt sample. Evidence of a new
state is found with a local significance of , whose mass and width
are measured to be and , respectively. In addition, evidence of a new decay mode
is found with a significance of
. The relative branching fraction of with respect to the
decay is measured to be , where the first
uncertainty is statistical, the second systematic and the third originates from
the branching fractions of charm hadron decays.Comment: All figures and tables, along with any supplementary material and
additional information, are available at
https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-028.html (LHCb
public pages
Measurement of the ratios of branching fractions and
The ratios of branching fractions
and are measured, assuming isospin symmetry, using a
sample of proton-proton collision data corresponding to 3.0 fb of
integrated luminosity recorded by the LHCb experiment during 2011 and 2012. The
tau lepton is identified in the decay mode
. The measured values are
and
, where the first uncertainty is
statistical and the second is systematic. The correlation between these
measurements is . Results are consistent with the current average
of these quantities and are at a combined 1.9 standard deviations from the
predictions based on lepton flavor universality in the Standard Model.Comment: All figures and tables, along with any supplementary material and
additional information, are available at
https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2022-039.html (LHCb
public pages
Real Time Detection of Soil Moisture in Winter Jujube Orchard Based on NIR Spectroscopy
International audienceThe measurement and control of soil moisture are the key technologies of precision agriculture. In order to real-time detect soil moisture content faster and more accurately, a portable soil moisture sensor based on NIR spectroscopy was developed. With the sixty soil samples collected from a winter jujube orchard, a linear regression model was established. The determination coefficients of the calibration () and validation () reached 0.88 and 0.92, respectively. The model passed F-test and t-test and showed robust. Subsequently, two spatial distribution maps of soil moisture were generated based on the data obtained by the portable soil moisture detector and the data obtained by oven drying method, respectively. Finally, the correlation between these two maps was investigated by using the software of Surfer 8.0. The zones of dry and wet soil could be distinguished easily in both maps. The results of the study showed that the developed detector was practical
SiBCN-ZrO2 hybrid ceramic aerogels through the polymer-derived ceramics (PDCs) route
The SiBCN-ZrO2 hybrid ceramic aerogels were fabricated by pyrolysis of preceramic aerogels prepared from Polyborosilane/Zirconium 5n-butoxide /Divinylbenzene (PSNB/ZBO/DVB) combining a simple hydrosilylation reaction with CO2 supercritical drying. The micromorphology and pore structure parameters of the preceramic hybrid aerogels were investigated. Structural evolution of the ceramic hybrid aerogels and the crystallization behavior were studied by scanning electron microscope (SEM), X-ray diffraction (XRD) and transmission electron microscopy (TEM). The results showed that all the preceramic hybrid aerogels had a typical mesoporous structure. The neck region between the particles of SiBCN-ZrO2 ceramic hybrid aerogels was gradually increased when the sintering temperatures increased from 750 °C to 1550 °C. After heat treatment at 1550 °C, the specific surface area and pore volume for the samples were drastically reduced down to 108.85 m2 gâ1 and 0.49 cm3 gâ1, respectively. The ZrO2 nanocrystals were well-distributed in amorphous SiBCN network backbone, the final crystalline phases were composed of hexagonal BN, ÎČ-SiC crystals and monoclinic ZrO2.Accepted versio
Non-Contact Electric Field-Enhanced Abnormal Grain Growth In (K0.5Na0.5)Nbo3 Ceramics
The effect of a non-contact electric field on the sintering behavior of (K0.5Na0.5)NbO3 (KNN) ceramics was studied. The results revealed that the electric field can enhance the densification and grain growth, and promote abnormal grain growth in KNN ceramics. The phenomena are likely because the electric field promoted the formation of liquid phase, leading to enhanced mass transport. A simple thermodynamic model was proposed to explain the solid-liquid phase transformation under an electric field
Genome Survey Sequencing of Luffa Cylindrica L. and Microsatellite High Resolution Melting (SSR-HRM) Analysis for Genetic Relationship of Luffa Genotypes
Luffa cylindrica (L.) Roem. is an economically important vegetable crop in China. However, the genomic information on this species is currently unknown. In this study, for the first time, a genome survey of L. cylindrica was carried out using next-generation sequencing (NGS) technology. In total, 43.40 Gb sequence data of L. cylindrica, about 54.94Ă coverage of the estimated genome size of 789.97 Mb, were obtained from HiSeq 2500 sequencing, in which the guanine plus cytosine (GC) content was calculated to be 37.90%. The heterozygosity of genome sequences was only 0.24%. In total, 1,913,731 contigs (>200 bp) with 525 bp N50 length and 1,410,117 scaffolds (>200 bp) with 885.01 Mb total length were obtained. From the initial assembled L. cylindrica genome, 431,234 microsatellites (SSRs) (â„5 repeats) were identified. The motif types of SSR repeats included 62.88% di-nucleotide, 31.03% tri-nucleotide, 4.59% tetra-nucleotide, 0.96% penta-nucleotide and 0.54% hexa-nucleotide. Eighty genomic SSR markers were developed, and 51/80 primers could be used in both âZheda 23â and âZheda 83â. Nineteen SSRs were used to investigate the genetic diversity among 32 accessions through SSR-HRM analysis. The unweighted pair group method analysis (UPGMA) dendrogram tree was built by calculating the SSR-HRM raw data. SSR-HRM could be effectively used for genotype relationship analysis of Luffa species
Bioremoval efficiency and metabolomic profiles of cellular responses of Chlorella pyrenoidosa to phenol and 4-fluorophenol
We examined the growth tolerance, bioremoval efficacy, and metabolomic profiles of the cellular responses of Chlorella pyrenoidosa to phenol and 4-fluorophenol. We found that C. pyrenoidosa can tolerate exposure to 100 mg/L of phenol and 4-fluorophenol, and the growth of algal cells had a significant hormesis of inhibition first and then promotion. Up to 70% bioremoval of phenol and 4-fluorophenol may occur after 240 h of treatment. Superoxide dismutase (SOD) and catalase (CAT) activities, malondialdehyde (MDA) content, and reactive oxygen species (ROS) in algal cells in the phenol- and 4-fluorophenol-treated groups were similar or lower than in the control group. Furthermore, photosynthetic pigment and glycerophospholipid contents were significantly upregulated in both phenol- and 4-fluorophenol-treated groups, as indicated by the metabolomic analysis of C. pyrenoidosa, resulting in the vigorous growth of algal cells compared to the control group. Therefore, C. pyrenoidosa can be an excellent biosorbent for phenol and 4-fluorophenol.
HIGHLIGHTS
Chlorella pyrenoidosa can well tolerate 100 mg/L of phenol or 4-fluorophenol exposure.;
100 mg/L phenol or 4-fluorophenol can be bioremoved by >70% after 240 h of treatment.;
Significant hormesis in algal growth was observed during the treatment.;
Growth promotion is attributed to chlorophyll and glycerophospholipid accumulation.
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