6 research outputs found
Transcriptional profiling of interleukin-2-primed human adipose derived mesenchymal stem cells revealed dramatic changes in stem cells response imposed by replicative senescence
Inflammation is a double-edged sword with both detrimental and
beneficial consequences. Understanding of the mechanisms of crosstalk
between the inflammatory milieu and human adult mesenchymal stem cells
is an important basis for clinical efforts. Here, we investigate changes
in the transcriptional response of human adipose-derived stem cells to
physiologically relevant levels of IL-2 (IL-2 priming) upon replicative
senescence. Our data suggest that replicative senescence might
dramatically impede human mesenchymal stem cell (MSC) function via
global transcriptional deregulation in response to IL-2. We uncovered a
novel senescence-associated transcriptional signature in human
adipose-derived MSCs hADSCs after exposure to pro-inflammatory
environment: significant enhancement of the expression of the genes
encoding potent growth factors and cytokines with anti-inflammatory and
migration-promoting properties, as well as genes encoding angiogenic and
antiapoptotic promoting factors, all of which could participate in the
establishment of a unique microenvironment. We observed transcriptional
up-regulation of critical components of the nitric oxide synthase
pathway (iNOS) in hADSCs upon replicative senescence suggesting, that
senescent stem cells can acquire metastasis-promoting properties via
stem cell-mediated immunosuppression. Our study highlights the
importance of age as a factor when designing cell-based or
pharmacological therapies for older patients and predicts measurable
biomarkers characteristic of an environment that is conducive to cancer
cells invasiveness and metastasis.LM and BGG was supported by grants from the Spanish Ministry of Science
and Innovation (SAF 2010-15239) to BGG and. LMP are supported by FPI
fellowships from the Spanish Ministry, and BGG acknowledges support from
the ``Ramon y Cajal´´ tenure track programme from the Spanish Ministry
of Science and Innovation (RYC2009-04669). AS and AA are fellows of
Bolashak International Scholarship, AA, AN, AS are sponsored by KazNMU
sponsored program.S
HCH Removal in a Biochar-Amended Biofilter
This study evaluated the efficiency of two biofilter systems, with and without biochar chambers installed, at degrading and removing HCH and its isomers in natural drainage water. The biochar biofilter proved to be 96% efficient at cleaning HCH and its transformation products from drainage water, a significant improvement over classic biofilter that remove, on average, 68% of HCH. Although iron- and sulfur-oxidizing bacteria, such as Gallionella and Sulfuricurvum, were dominant in the biochar bed outflows, they were absent in sediments, which were rich in Simplicispira, Rhodoluna, Rhodoferax, and Flavobacterium. The presence of functional genes involved in the biodegradation of HCH isomers and their byproducts was confirmed in both systems. The high effectiveness of the biochar biofilter displayed in this study should further encourage the use of biochar in water treatment solutions, e.g., for temporary water purification installations during the construction of other long-term wastewater treatment technologies, or even as final solutions at contaminated sites
Phytoremediation potential of Miscanthus sinensis And. in organochlorine pesticides contaminated soil amended by Tween 20 and Activated carbon.
Assessing HCH isomer uptake in Alnus glutinosa: implications for phytoremediation and microbial response
Abstract Although the pesticide hexachlorocyclohexane (HCH) and its isomers have long been banned, their presence in the environment is still reported worldwide. In this study, we investigated the bioaccumulation potential of α, β, and δ hexachlorocyclohexane (HCH) isomers in black alder saplings (Alnus glutinosa) to assess their environmental impact. Each isomer, at a concentration of 50 mg/kg, was individually mixed with soil, and triplicate setups, including a control without HCH, were monitored for three months with access to water. Gas chromatography–mass spectrometry revealed the highest concentrations of HCH isomers in roots, decreasing towards branches and leaves, with δ-HCH exhibiting the highest uptake (roots—14.7 µg/g, trunk—7.2 µg/g, branches—1.53 µg/g, leaves—1.88 µg/g). Interestingly, α-HCH was detected in high concentrations in β-HCH polluted soil. Phytohormone analysis indicated altered cytokinin, jasmonate, abscisate, and gibberellin levels in A. glutinosa in response to HCH contamination. In addition, amplicon 16S rRNA sequencing was used to study the rhizosphere and soil microbial community. While rhizosphere microbial populations were generally similar in all HCH isomer samples, Pseudomonas spp. decreased across all HCH-amended samples, and Tomentella dominated in β-HCH and control rhizosphere samples but was lowest in δ-HCH samples
Prevalence of Rickettsia species in ticks including identification of unknown species in two regions in Kazakhstan
Abstract Background Over 60 years ago clinical patterns resembling tick-borne rickettsioses have been described for the first time in Kazakhstan. Since 1995 the incidence of clinical cases of tick-borne rickettsioses in humans seems to be rising but studies on epidemiological data regarding the occurring etiological agents, tick vector species, prevalence and distribution throughout Kazakhstan are still scarce to date. The aim of the study was molecular investigation of ticks for spotted-fever group rickettsiae in the endemic Kyzylorda region and the so far considered as non-endemic Almaty region. A total of 2341 ticks was collected in the two regions in Kazakhstan and sorted in 501 pools: Ixodes persulcatus (243); Dermacentor marginatus (129); Haemaphysalis punctata (104); Hyalomma asiaticum (17); Dermacentor reticulatus (3); and Rhipicephalus turanicus (5). Pools were tested for Rickettsia spp. using real-time PCR. For positive samples multilocus sequence typing (MLST) was performed. Results The calculated minimum infection rate (MIR) for rickettsiae in the investigated ticks in Almaty region varied between 0.4–15.1% and 12.6–22.7% in the Kyzylorda region. At least four different Rickettsia species were identified in the two selected regions of Kazakhstan. Two of these are already known to science: Rickettsia raoultii and R. slovaca, the latter being reported for the first time in Almaty region One new form, “Candidatus R. yenbekshikazakhensis”, was described by MLST of six gene fragments in Almaty region and one new genotype, “genotype R. talgarensis” was detected using three gene fragments. Conclusions Kazakh physicians should be aware of rickettsioses after tick bites in both regions studied. Both, R. raoultii and R. slovaca should be included in the diagnostics. The role for human diseases has further to be investigated for the newly described rickettsiae, “Candidatus R. yenbekshikazakhensis” and “Genotype R. talgarensis”