Evaluating Management Options to Increase Roadside Carbon Sequestration

We estimated the amount of carbon sequestered along Montana Department of Transportation (MDT) roads and tested 3 different highway right-of-way (ROW) management techniques to increase carbon stocks. Using Geographic Information System techniques, the total ROW acreage owned by MDT was found to sequester 75,292 metric tons of carbon per year and to consist mostly of grasslands (70%). From 2016-2018 we tested 3 ROW management techniques to increase carbon stocks- increase mowing height, plant woody shrubs, or add legumes to reclamation seed mixes of disturbed soils - at 3 sites (Three Forks [3F], Bear Canyon [BC], and Bozeman Pass [BP]) along Interstate 90 in southwestern Montana. Soil samples generally averaged 0.75–1.5% soil organic carbon (SOC) at the 3F site, 2.5–4% SOC at the BC site, and 1.5–2.5% SOC at the BP site. Average SOC levels were always lower in 2018 than in 2016. Soil respiration rates were generally highest in June or July at the BC site, averaging ~4 μmol CO2 m-2 second-1. Soil respiration rates were lower at the BC site in November 2016, at the BP site in June 2018, and at the 3F site in July 2018 (all ~2–3 μmol CO2 m-2 s-1). Aboveground biomass carbon estimates generally mirrored belowground SOC estimates. Taken together, our findings suggest that of the three treatments implemented (raised mowing height, shrub planting, and disturbance), minimizing disturbance to soils likely makes the greatest contribution to the medium- and long-term carbon-storage potential of these roadside soils

Constitutional Law - Compulsory Medical Treatment for an Adult - The Right of an Adult to Refuse Lifesaving Medical Treatment Based upon the Free Exercise Clause of the First Amendment

The Supreme Court of New Jersey has held that an adult, whose religious beliefs forbid blood transfusions but who risks death if a transfusion is not given, may be compelled to submit to such treatment. John F. Kennedy Memorial Hospital v. Heston, 58 N.J. 576, 279 A.2d 670 (1971)

Bees and Butterflies in Roadside Habitats: Identifying Patterns, Protecting Monarchs, and Informing Management

Pollinating insects provide vital ecosystem services and are facing global declines and habitat loss. Roadsides are increasingly regarded as important potential areas for enhancing pollinator habitat. Understanding which roadsides best support pollinators\u2014and why\u2014is essential to helping locate and prioritize pollinator conservation efforts across roadside networks. To support this effort, we assessed butterfly, bee, and flowering plant species richness and abundance on a set of 63 stratified randomized roadside transects in State-managed rights-of-way in SE Idaho. Our research evaluated pollinator diversity as a function of highway class (interstate, U.S., and state highways), remotely sensed NDVI values (a measure of vegetation greenness), and floral resources. We found that smaller highways and lower (less green) maximum NDVI values were associated with significantly more bee species and total bees. Roadsides bordering sagebrush habitats typically had low NDVI values and higher bee and butterfly species richness, potentially contributing to this observed pattern. Butterfly richness increased in association with higher floral abundance in roadsides. Additionally, we identified and mapped 1,363 roadside patches of milkweed (Asclepias speciosa), larval host plant for the imperiled monarch butterfly (Danaus plexippus), in a survey of over 900 miles of southern Idaho highways. Based on these results and a literature review, we recommend management strategies to promote the health of pollinator populations in Idaho\u2019s rights-of-way and provide data to help ITD prioritize areas for pollinator-friendly management practices and habitat restoration within their highway system

Mutant Neurogenin-3 in congenital malabsorptive diarrhea

Background: Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine-cell development in the pancreas and intestine. The NEUROG3 gene (NEUROG3) is therefore a candidate for the cause of a newly discovered autosomal recessive disorder characterized by generalized malabsorption and a paucity of enteroendocrine cells. Methods: We screened genomic DNA from three unrelated patients with sparse enteroendocrine cells for mutations of NEUROG3. We then tested the ability of the observed mutations to alter NEUROG3 function, using in vitro and in vivo assays. Results: The patients had few intestinal enteroendocrine cells positive for chromogranin A, but they had normal numbers of Paneth\u27s, goblet, and absorptive cells. We identified two homozygous mutations in NEUROG3, both of which rendered the NEUROG3 protein unable to activate NEUROD1, a downstream target of NEUROG3, and compromised the ability of NEUROG3 to bind to an E-box element in the NEUROD1 promoter. The injection of wild-type but not mutant NEUROG3 messenger RNA into xenopus embryos induced NEUROD1 expression. Conclusions: A newly discovered disorder characterized by malabsorptive diarrhea and a lack of intestinal enteroendocrine cells is caused by loss-of-function mutations in NEUROG3. Copyright © 2006 Massachusetts Medical Society

Rare variants implicate NMDA receptor signaling and cerebellar gene networks in risk for bipolar disorder

Bipolar disorder is an often-severe mental health condition characterized by alternation between extreme mood states of mania and depression. Despite strong heritability and the recent identification of 64 common variant risk loci of small effect, pathophysiological mechanisms remain unknown. Here, we analyzed genome sequences from 41 multiply-affected pedigrees and identified variants in 741 genes with nominally significant linkage or association with bipolar disorder. These 741 genes overlapped known risk genes for neurodevelopmental disorders and clustered within gene networks enriched for synaptic and nuclear functions. The top variant in this analysis - prioritized by statistical association, predicted deleteriousness, and network centrality - was a missense variant in the gene encoding D-amino acid oxidase (DAOG131V). Heterologous expression of DAOG131V in human cells resulted in decreased DAO protein abundance and enzymatic activity. In a knock-in mouse model of DAOG131, DaoG130V/+, we similarly found decreased DAO protein abundance in hindbrain regions, as well as enhanced stress susceptibility and blunted behavioral responses to pharmacological inhibition of N-methyl-D-aspartate receptors (NMDARs). RNA sequencing of cerebellar tissue revealed that DaoG130V resulted in decreased expression of two gene networks that are enriched for synaptic functions and for genes expressed, respectively, in Purkinje neurons or granule neurons. These gene networks were also down-regulated in the cerebellum of patients with bipolar disorder compared to healthy controls and were enriched for additional rare variants associated with bipolar disorder risk. These findings implicate dysregulation of NMDAR signaling and of gene expression in cerebellar neurons in bipolar disorder pathophysiology and provide insight into its genetic architecture

Evaluating Management Options to Increase Roadside Carbon Sequestration

DTRT13-G-UTC49We estimated the amount of carbon sequestered along Montana Department of Transportation (MDT) roads and tested 3 different highway right-of-way (ROW) management techniques to increase carbon stocks. Using Geographic Information System techniques, the total ROW acreage owned by MDT was found to sequester 75,292 metric tons of carbon per year and to consist mostly of grasslands (70%). From 2016-2018 we tested 3 ROW management techniques to increase carbon stocks- increase mowing height, plant woody shrubs, or add legumes to reclamation seed mixes of disturbed soils - at 3 sites (Three Forks [3F], Bear Canyon [BC], and Bozeman Pass [BP]) along Interstate 90 in southwestern Montana. Soil samples generally averaged 0.75\u20131.5% soil organic carbon (SOC) at the 3F site, 2.5\u20134% SOC at the BC site, and 1.5\u20132.5% SOC at the BP site. Average SOC levels were always lower in 2018 than in 2016. Soil respiration rates were generally highest in June or July at the BC site, averaging ~4 \u3bcmol CO2 m-2 second-1. Soil respiration rates were lower at the BC site in November 2016, at the BP site in June 2018, and at the 3F site in July 2018 (all ~2\u20133 \u3bcmol CO2 m-2 s-1). Aboveground biomass carbon estimates generally mirrored belowground SOC estimates. Taken together, our findings suggest that of the three treatments implemented (raised mowing height, shrub planting, and disturbance), minimizing disturbance to soils likely makes the greatest contribution to the medium- and long-term carbon-storage potential of these roadside soils

The barriers and facilitators influencing the sustainability of hospital-based interventions: a systematic review

Acknowledgements University of Stirling for providing financial support for open access costs Funding This review was funded by the Chief Scientist Office, grant number GCA/17/26. JC, PC and EAD are employed by the Nursing, Midwifery and Allied Health Professions Research Unit, which is funded by the Chief Scientist Office in Scotland.Peer reviewedPublisher PD

Transcriptional regulatory networks underlying gene expression changes in Huntington's disease

Abstract Transcriptional changes occur presymptomatically and throughout Huntington's disease (HD), motivating the study of transcriptional regulatory networks (TRNs) in HD. We reconstructed a genome‐scale model for the target genes of 718 transcription factors (TFs) in the mouse striatum by integrating a model of genomic binding sites with transcriptome profiling of striatal tissue from HD mouse models. We identified 48 differentially expressed TF‐target gene modules associated with age‐ and CAG repeat length‐dependent gene expression changes in Htt CAG knock‐in mouse striatum and replicated many of these associations in independent transcriptomic and proteomic datasets. Thirteen of 48 of these predicted TF‐target gene modules were also differentially expressed in striatal tissue from human disease. We experimentally validated a specific model prediction that SMAD3 regulates HD‐related gene expression changes using chromatin immunoprecipitation and deep sequencing (ChIP‐seq) of mouse striatum. We found CAG repeat length‐dependent changes in the genomic occupancy of SMAD3 and confirmed our model's prediction that many SMAD3 target genes are downregulated early in HD