Chtonobdella tanae sp. n. holotype (AU81) from Queensland Australia, μCT projections.

Specimen fixed in 10% formalin and 70% ethanol for over a decade was refixed with AFA, stained in osmium tetroxide, and then scanned in a GE v|tome|x s240

Chtonobdella tanae sp. n. holotype (AU81) from Queensland Australia, μCT volumetric video rendering of external and internal features.

Video starts with whole-body (anterior to the right) external feature rendering viewed dorsally, laterally, and then ventrally, followed by whole-body internal feature rendering viewed dorsally, laterally, and then ventrally. Video zooms in on eyespots (green), jaws (magenta), ganglia (yellow), and gastric tissue (grey) viewed dorsally, laterally, and then ventrally. Video moves posteriorly, focusing on male median reproductive tissues (shades of blue), female median reproductive tissues (pink), and testisacs (red) viewed dorsally, laterally, and then ventrally. Video goes to the posterior focusing on intestinal tissue (pale green) viewed dorsally, laterally, and then ventrally. Video then focuses on the auricle cavities (blue), finishing on the dorsal view of whole-body internal feature

Juvenile Macrobdella decora, μCT projections.

Specimens were fixed first with either ethanol (small sized specimen on left), glutaraldehyde (large sized specimen in middle), or AFA (medium sized specimen on right); stained in osmium tetroxide; and then scanned in a GE v|tome|x s240