Utjecaj morfološkog tipa stanica na ekspresiju bcl-2, Ki-67 i p-53 u uvealnim melanomima

Uveal melanomas are known to be less aggressive malignant tumors compared with melanoma of other localizations but the same morphological characteristics. We hypothesized that immunohistochemical determination of expression of the known proliferation markers bcl-2 and ki-67, and p-53 as a suppressor gene marker, could better explain the biological behavior of uveal melanoma vs. melanoma of other localizations. Thirty cases of primary uveal melanoma of two levels of invasion were tested retrospectively for the presence of bcl-2, Ki-67 and p-53 proteins with the indirect peroxidase-antiperoxidase immunoenzyme method and three step ABC/AP method. The intensity of reaction was assessed by semiquantiative method.Usprkos morfološkoj građi sličnoj drugim melanomima, poznato je da uvealni melanomi imaju daleko bolju prognozu od melanoma drugih lokalizacija. Upravo stoga pretpostavili smo da bi određivanje ekspresije bcl-2, Ki-67 kao poznatih čimbenika proliferacije, te p-53 proteina u stanicama uvealnih melanoma moglo protumačiti ovakvo biološko ponašanje uvealnih melanoma. U tu svrhu ispitano je retrogradno 30 primarnih uvealnih melanoma stadija pT2 i pT3 na prisutnost bcl-2, Ki-67 i p-53 proteina indirektnom metodom peroksidaze-anti-peroksidaze i trofaznom metodom ABC/AP. Stupanj reaktivnosti određen je semikvantitativnom metodom

Histološka različitost u koštanoj biopsiji postmenopauzalnih i osteoporotičnih žena

Many investigations have been carried out on osteoporosis and showed this disorder in a new light. The reduction in bone structure and dynamics points to the etiology that may be of therapeutic significance. Today we still do not know everything about the histomorphometric characteristics of each metabolic disturbance and bone disease. Bone biopsy and histomorphometry are not available for most patients and that is the reason why a broad classification of these reductions in osteoporosis and osteomalacia are widely accepted in clinical practice. New findings from this complex domain are necessary to design a strategy in the evolution of new therapeutic devices and drugs with the intention to decrease the high disability and mortality rate in the vulnerable population of postmenopausal women.Brojna istraživanja provedena posljednjih godina pokazala su osteoporozu u novom svjetlu. Promjene u koštanoj strukturi i dinamici upućuju na etiologiju poremećaja, što je od presudnog značenja za liječenje bolesti. S obzirom na činjenicu da još uvijek malo znamo o histomorfometrijskim značajkama pojedinih metaboličnih bolesti te da je biopsija kosti i histomorfometrija još uvijek teže dostupna većem broju bolesnika, danas se u kliničkoj praksi uglavnom rabi klinička klasifikacija po kojoj se one dijele u dvije široke kategorije: osteoporozu i osteomalaciju. Nova saznanja iz ovoga područja su od presudne važnosti za planiranje buduće strategije razvoja novih terapijskih sredstava i lijekova kako bi se smanjio visok postotak invaliditeta i smrtnosti u osjetljivoj populaciji postmenopauzalnih žena

Studies in the Sphingolipids Series. XI. Synthesis of l-14C-hydroxy-2-aminooctadecane (DL_l- 14C-Sphingine)

The preparation of a 14C-labeled sphingine is described. Radioactive carbon was introduced by the treatment of heptadecanoic acid chloride with C-diazomethane, prepared from C-methyl iodide (1 mC). The obtained ketone was converted via 1-C-acetoxy-octadecan-2-one and l-C-hydroxyoctadecan-2-one oxime into 1-14C-hydroxy-2 aminooctadecane (spec. activity: 33,.48 μC /mM). The over-all radiochemical yield based on 14C-methylidide was 12,15%

Some Slaughter and Carcass Traits of the Lambs of Dalmatian Pramenka Reared in Three Different Fattening Systems

In order to determine the impact of different fattening system (I - milk, pasture; II - indoors, milk, grains, III - milk, pasture, concentrate) on slaughter value and carcass quality of the lambs of Dalmatian Pramenka, 18 lambs (100 ± 5 days old), 6 in each group, were slaughtered. Lambs of one group were from the same flock and selected by birth weight (2.00 ± 0.20 kg). After slaughtering and cutting the carcasses into halves, in order to determine the share of legs and shoulders as well as certain tissues in the halves, the legs and shoulders were separated from the halves and total dissection was made. The significant differences (P<0.05) of slaughter weight (I - 21.17 kg, II - 23.25 kg, III - 26.25 kg) and hot carcass weight (I - 9.98 kg, II - 11.92 kg, III - 12.92 kg) among three groups were found. The legs II (1.65 kg) and III (1.71 kg) were significantly heavier (P<0.001) than legs I (1.32 kg), as well as shoulders (I - 0.52 kg, II - 0.65 kg, III - 0.69 kg; P<0.01). Total dissection of the halves established these tissues ratio: muscle 51.25 %, fat 10.18 %, connective 13.93%, bone 23.04% and other tissues 2.32%. The biggest quantity of muscle tissue was found in halves III (3.27 kg) what was more (P<0.05) than in halves II (2.83 kg) and I (2.50 kg). However, the biggest quantity of fat was found in halves II (0.85 kg) what was more (P<0.01) than in halves I (0.33 kg) and III (0.52 kg). Therefore, the addition of concentrate in pasture fattening system (III) increased the muscularity, without significantly increasing the amount of fat in the lamb carcass