The mechanisms of proanthocyanidins Inhibits PGE_2 synthesis in lipopolysaccharide-Induced RAW264.7 cells

目的:观察原花青素对脂多糖诱导rAW264.7细胞PgE2生成的影响及其作用机制。方法:放射免疫法(rIA)检测原花青素对脂多糖诱导的rAW264.7细胞PgE2生成的影响;lPS诱导rAW264.7细胞9H,再加不同浓度原花青素作用30MIn,放射免疫(rIA)法检测原花青素对COX-2酶活性的影响;半定量逆转录-聚合酶联反应(rT-PCr)法检测原花青素对COX-2 MrnA表达的影响;提取核蛋白,蛋白免疫印迹(WESTErn blOT)法检测原花青素对nf-κb/P65蛋白表达的影响;电泳迁移率变动分析(EMSA)法检测原花青素对nf-κb与dnA结合活性的影响。结果:0.8,4和20Mg·l-1原花青素抑制lPS诱导rAW264.7细胞PgE2生成;0.8,4和20Mg·l-1原花青素不影响lPS诱导rAW264.7细胞COX-2酶活性;0.8,4和20Mg·l-1原花青素下调lPS诱导rAW264.7细胞COX-2 MrnA表达;4,20Mg·l-1原花青素下调lPS诱导rAW264.7细胞nf-κb/P65蛋白表达;0.8,4和20Mg·l-1的原花青素可明显降低lPS诱导下rAW264.7细胞的nf-κb活化。结论:原花青素显著抑制lPS诱导rAW264.7细胞PgE2生成的作用与原花青素抑制COX-2 MrnA表达有关,此作用可能是通过抑制nf-κb/P65蛋白表达和抑制nf-κb的dnA结合活性来实现。Objective:To study the effect of proanthocyanidins on PGE2 synthesis in lipopolysaccharide-induced RAW264.7 cells and its mechanisms.Methods: The effect of proanthocyanidins on PGE2 synthesis in lipopolysaccharide-induced RAW264.7 cells was measured by radioimmunoassay(RIA);After being pretreated with different concentrations of proanthocyanidins for 30 min,and then LPS 1 mg·L-1 for 9 h,the effect of proanthocyanidins on the activity of COx-2 enzyme in RAW264.7 cells was analysed by RIA;the expressions of COx-2 mRNA by RT-PCR;the nuclear protein was isolated and the expressions of NF-κB protein by Western blot;and the DNA-binding activity of NF-κB was measured by electrophoretic mobility shift assay(EMSA).Results: PGE2 synthesis was inhibited by proanthocyanidins 0.8 mg·L-1、4 mg·L-1 and 20 mg·L-1 ;the activity of COx-2 enzyme was not inhibited by proanthocyanidins 0.8 mg·L-1、4 mg·L-1 and 20 mg·L-1 (P>0.05,vs LPS group);the expression of COx-2 mRNA was inhibited by proanthocyanidins 0.8 mg·L-1、4 mg·L-1 and 20 mg·L-1;the expression of NF-κB/p65 protein was inhibited by proanthocyanidins 4 mg·L-1 and 20 mg·L-1.The DNA-binding activity of NF-κB was reduced by proanthocyanidins 0.8 mg·L-1、4 mg·L-1 and 20 mg·L-1.Conclusion: The mechanisms of proanthocyanidins inhibited PGE2 synthesis in lipopolysaccharide-induced RAW264.7 cells is related to the inhibition of the expression of COx-2 mRNA ,which is inhibited possibly by suppressing expression of NF-κB/p65 protein and DNA-binding activity of NF-κB.广东省教育厅自然科学基金(Z03045)---

α-羟丁酸脱氢酶与乳酸脱氢酶比值的临床意义

目的探讨α-羟丁酸脱氢酶与乳酸脱氢酶比值对心肌梗死的诊断价值。方法采用470例血清HBDH升高患者血清,分为心肌梗死组、脑部疾病组、肺部疾病组、肝脏疾病组、肾脏疾病组,测定血清中α-羟丁酸脱氢酶与乳酸脱氢酶比值。同时,设正常对照组。结果心肌梗塞组及脑部疾病组HBDH/LDH比值明显升高,与正常对照组有着显著性差异(P0.05)。结论以HBDH/LDH的比值诊断心肌梗塞较之单纯用HBDH或LDH诊断特异性高

The effect of kaempferol on COX-2and iNOS expression and generated production in LPS-induced RAW 264.7 cells

目的探讨山萘酚对脂多糖(lIPOPOlySACCHArIdES,lPS)诱导rAW 264.7细胞COX-2及InOS表达的影响。方法四氮唑盐法(MOnOTE-TrAzOlIuM TEST,MTT)检测山奈酚对rAW264.7细胞生长增殖的影响,放射免疫测定法(rIA)检测山萘酚对PgE2和nO生成的影响,免疫印迹法(WESTErn blOTTIng)检测COX-2及InOS蛋白的表达。结果山萘酚抑制lPS诱导的rAW 264.7细胞PgE2和nO的生成,同时下调lPS诱导的rAW 264.7细胞COX-2及InOS蛋白的表达。结论山萘酚抑制2个诱导酶COX-2和InOS的表达,从而减少炎性产物PgE2和nO的生成,这可能是山萘酚抗炎的机制之一。Objective To study the effect of kaempferol on COX-2and iNOS expression in LPS-induced RAW 264.7cells.Methods The effects of kaempferol on the proliferation in RAW264.7cells were evaluated by monote-trazolium test assay(MTT);the effect o f kaempferol on the product of PGE2and NO in RAW264.7cells was measured by radioimmunoassay(RIA);the effects of kaempferol on the expressions of COX-2and iNOS protein in RAW264.7cells were analyzed by Western blot.Results Kaempferol inhibited the LPS-induced PGE2and NO synthesis in RAW 264.7cells.The protein expression of COX-2and iNOS in RAW264.7cells were also decreased by kaempferol.Conclusions The results show that kaempferol reduces the augmented biosynthesis of PGE2and NO by inhibiting the protein expression of COX-2and iNOS in RAW264.7cells.The finding may partly explain the anti-inflammatory effect of kaempferol.广东省社会发展领域科技计划项目(53025); 广东省重点扶持学科项目(GX0307

一种生产生物油脂和生物柴油的方法

本发明涉及生物能源领域，具体地说是一种生产生物油脂和生物柴油的方法，以海洋滩涂植物大米草为原料，采用酵母工程菌将大米草分步糖化发酵生产生物油脂和生物柴油。本发明是一种由海洋滩涂植物大米草经酸水解产糖后利用微生物中的酵母工程菌发酵转化为油脂或柴油的新工艺，该工艺产油率高、转化快，在变废为宝和环境保护等方面具有广阔的应用前景。带填